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Article

IL-15, in synergy with RAE-1ε, stimulates TCR‑independent proliferation and activation of CD8+ T cells

  • Authors:
    • Li Qian
    • Yue Zhang
    • Xin-Yuan Pan
    • Ming-Chun Ji
    • Wei-Juan Gong
    • Fang Tian
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, School of Medicine, Yangzhou University, Yangzhou 225001, P.R. China, Department of General Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China
  • Pages: 472-476
    |
    Published online on: November 25, 2011
       https://doi.org/10.3892/ol.2011.495
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Abstract

CD8+ T cells play critical roles in immunosurveil­lance by killing malignant or virally infected cells. Interleukin 15 (IL-15) is a critical cytokine for promoting proliferation and the effector capacity of CD8+ T cells, and has been used to support the growth of CD8+ T cells in cellular therapies of neoplastic diseases. Recent studies have shown that IL-15, in synergy with other cytokines, such as IL-6, enhances the T-cell receptor (TCR)-independent proliferation and function of CD8+ T cells. The aim of the present study was to investigate the role of BaF3-mb15-RAE cells in stimulating mouse CD8+ T cells. BaF3 cells were cultured and B16F10 cells were grown in DMEM. MTT assay was used to detect the proliferation of CD8+ T cells. Cells were analyzed using flow cytometry. The results showed that IL-15 synergistically acts with another T-cell stimulatory molecule, RAE1ε, to potently promote the proliferation of CD8+ T cells, induce CD8+ T-cell activation and enhance granzyme B and interferon-γ (IFN-γ) production in the absence of signaling via the TCR. Moreover, IL-15 in combination with RAE1ε resulted in a cooperative effect on CD8+ T-cell-mediated cytotoxicity against B16F10 tumor cells. Thus, results of the present study showed that IL-15, in synergy with RAE1ε, enhances the TCR-independent effector function of CD8+ T cells in vitro, which may be useful in the cellular immunotherapy of cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Qian L, Zhang Y, Pan X, Ji M, Gong W and Tian F: IL-15, in synergy with RAE-1ε, stimulates TCR‑independent proliferation and activation of CD8+ T cells. Oncol Lett 3: 472-476, 2012.
APA
Qian, L., Zhang, Y., Pan, X., Ji, M., Gong, W., & Tian, F. (2012). IL-15, in synergy with RAE-1ε, stimulates TCR‑independent proliferation and activation of CD8+ T cells. Oncology Letters, 3, 472-476. https://doi.org/10.3892/ol.2011.495
MLA
Qian, L., Zhang, Y., Pan, X., Ji, M., Gong, W., Tian, F."IL-15, in synergy with RAE-1ε, stimulates TCR‑independent proliferation and activation of CD8+ T cells". Oncology Letters 3.2 (2012): 472-476.
Chicago
Qian, L., Zhang, Y., Pan, X., Ji, M., Gong, W., Tian, F."IL-15, in synergy with RAE-1ε, stimulates TCR‑independent proliferation and activation of CD8+ T cells". Oncology Letters 3, no. 2 (2012): 472-476. https://doi.org/10.3892/ol.2011.495
Copy and paste a formatted citation
x
Spandidos Publications style
Qian L, Zhang Y, Pan X, Ji M, Gong W and Tian F: IL-15, in synergy with RAE-1ε, stimulates TCR‑independent proliferation and activation of CD8+ T cells. Oncol Lett 3: 472-476, 2012.
APA
Qian, L., Zhang, Y., Pan, X., Ji, M., Gong, W., & Tian, F. (2012). IL-15, in synergy with RAE-1ε, stimulates TCR‑independent proliferation and activation of CD8+ T cells. Oncology Letters, 3, 472-476. https://doi.org/10.3892/ol.2011.495
MLA
Qian, L., Zhang, Y., Pan, X., Ji, M., Gong, W., Tian, F."IL-15, in synergy with RAE-1ε, stimulates TCR‑independent proliferation and activation of CD8+ T cells". Oncology Letters 3.2 (2012): 472-476.
Chicago
Qian, L., Zhang, Y., Pan, X., Ji, M., Gong, W., Tian, F."IL-15, in synergy with RAE-1ε, stimulates TCR‑independent proliferation and activation of CD8+ T cells". Oncology Letters 3, no. 2 (2012): 472-476. https://doi.org/10.3892/ol.2011.495
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