SNRPE is involved in cell proliferation and progression of high-grade prostate cancer through the regulation of androgen receptor expression

Anchi,Takashi; Tamura,Kenji; Furihata,Mutsuo; Satake,Hirofumi; Sakoda,Hatsune; Kawada,Chiaki; Kamei,Maiko; Shimamoto,Tsutomu; Fukuhara,Hideo; Fukata,Satoshi; Ashida,Shingo; Karashima,Takashi; Yamasaki,Ichiro; Yasuda,Masaharu; Kamada,Masayuki; Inoue,Keiji; Shuin,Taro

doi:10.3892/ol.2011.505

February 2012 Volume 3 Issue 2

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February 2012 Volume 3 Issue 2

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SNRPE is involved in cell proliferation and progression of high-grade prostate cancer through the regulation of androgen receptor expression

Authors:
- Takashi Anchi
- Kenji Tamura
- Mutsuo Furihata
- Hirofumi Satake
- Hatsune Sakoda
- Chiaki Kawada
- Maiko Kamei
- Tsutomu Shimamoto
- Hideo Fukuhara
- Satoshi Fukata
- Shingo Ashida
- Takashi Karashima
- Ichiro Yamasaki
- Masaharu Yasuda
- Masayuki Kamada
- Keiji Inoue
- Taro Shuin
View Affiliations / Copyright

Affiliations: Department of Urology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan, Department of Pathology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan
Pages: 264-268
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Published online on: December 1, 2011

https://doi.org/10.3892/ol.2011.505
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Abstract

Clinically high-grade prostate cancers (PC) with high Gleason scores of 8-10 exhibit rapid growth and are more likely to spread beyond the prostate. These cancer types demonstrate a poor response to androgen deprivation therapy and eventually acquire a castration-resistant phenotype. To identify novel molecular cancer drug targets, we previously analyzed the gene expression profiles of high-grade PC using a cDNA microarray combined with laser microbeam microdissection and found a number of genes that are transactivated in high‑grade PC. Among these genes, we report the identification of a novel molecular target, small nuclear ribonucleoprotein polypeptide E (SNRPE). Semi-quantitative RT-PCR confirmed that SNRPE is overexpressed in high-grade PC cells compared with normal prostatic epithelial cells. Knockdown of SNRPE expression by short interfering RNA (siRNA) resulted in the marked suppression of PC cell proliferation. By contrast, SNRPE overexpression promoted PC cell proliferation, indicating its oncogenic effects. Furthermore, we demonstrated that SNRPE regulates androgen receptor (AR) mRNA expression in PC cells. Knockdown of SNRPE expression by siRNA resulted in the marked suppression of AR and its downstream target genes at the mRNA level. We suggest that the regulation of AR expression by SNRPE is essential for cell proliferation and progression of high-grade PC and that it may be a novel molecular target for cancer drugs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

SNRPE is involved in cell proliferation and progression of high-grade prostate cancer through the regulation of androgen receptor expression

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