Effects of 4-1BB signaling on the biological function of murine dendritic cells

Kuang,Youlin; Weng,Xiaodong; Liu,Xiuheng; Zhu,Hengchen; Chen,Zhiyuan; Chen,Hui

doi:10.3892/ol.2011.506

February 2012 Volume 3 Issue 2

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February 2012 Volume 3 Issue 2

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Article

Effects of 4-1BB signaling on the biological function of murine dendritic cells

Authors:
- Youlin Kuang
- Xiaodong Weng
- Xiuheng Liu
- Hengchen Zhu
- Zhiyuan Chen
- Hui Chen
View Affiliations / Copyright

Affiliations: Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, P.R. China
Pages: 477-481
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Published online on: December 1, 2011

https://doi.org/10.3892/ol.2011.506
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Abstract

4-1BB signaling has profound effects on the T cell-induced cell immune response, but its biological function in dendritic cells (DCs) has remained largely uncharacterized. In this study, we investigated the function of 4-1BB in murine DCs with an agonistic mAb to 4-1BB. Interleukin (IL)-6 and IL-12 production was assessed by an enzyme-linked immunosorbent assay (ELISA). Co-stimulatory molecules (CD80 and CD86) in DCs were analyzed by ﬂow cytometry. The results showed that 4-1BB was strongly expressed in DCs during the maturation process. Triggering 4-1BB increased the secretion of IL-6 and IL-12 and the upregulation of co-stimulatory molecules (CD80 and CD86) from DCs, indicating that agonistic mAb to 4-1BB directly improves the activation of DCs. Moreover, triggering 4-1BB induced a higher survival rate of DCs compared to that of hamster IgG isotype control, due to the upregulated expression of Bcl-2 and Bcl-xL. To further assess the role of 4-1BB on DCs stimulating T-cell proliferation, allogeneic mixed lymphocyte reactions were analyzed. The agonistic anti‑4-1BB mAb induced a higher T-cell proliferation. These results suggest that 4-1BB affects the duration, DC-T interaction and immunogenicity of DCs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of 4-1BB signaling on the biological function of murine dendritic cells

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