HGF stimulates proliferation through the HGF/c-Met pathway in nasopharyngeal carcinoma cells

  • Authors:
    • Rui Sun
    • Qing Zhang
    • Ling Guo
    • Ming-Yuan Chen
    • Ying Sun
    • Brian Cao
    • Jian Sun
  • View Affiliations

  • Published online on: February 20, 2012     https://doi.org/10.3892/ol.2012.613
  • Pages: 1124-1128
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Abstract

Hepatocyte growth factor (HGF) and its receptor c-Met are important in the development and homeostasis of a variety of human malignancies. However, the role of the HGF/c-Met signaling pathway in nasopharyngeal carcinoma (NPC) has not been clearly elucidated. This study examined the effect of HGF/c-Met on proliferation and migration in several NPC cell lines. RT-PCR was used to detect the HGF gene in CNE-1, CNE-2, HK-1, HONE-1 and SUNE-1 NPC cells. However, HGF gene expression was not detected in any of these cells. Using immunoblotting analysis, the Met25 protein was identified in HONE-1, HK-1 and CNE-1 cells. Results from fluorescence‑activated cell sorting (FACS) analysis revealed that anti-Met25 mAb specifically bound Met-expressing HONE-1, HK-1 and CNE-1 cells. It was further demonstrated that exogenous HGF was able to stimulate the proliferation of HONE-1 and HK-1 cells and the healing of scrape wounds in HONE-1 NPC cells. Our results reveal the potential therapeutic applications of combination therapy with antibodies targeting HGF in NPC patients.
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May 2012
Volume 3 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Sun R, Zhang Q, Guo L, Chen M, Sun Y, Cao B and Sun J: HGF stimulates proliferation through the HGF/c-Met pathway in nasopharyngeal carcinoma cells. Oncol Lett 3: 1124-1128, 2012
APA
Sun, R., Zhang, Q., Guo, L., Chen, M., Sun, Y., Cao, B., & Sun, J. (2012). HGF stimulates proliferation through the HGF/c-Met pathway in nasopharyngeal carcinoma cells. Oncology Letters, 3, 1124-1128. https://doi.org/10.3892/ol.2012.613
MLA
Sun, R., Zhang, Q., Guo, L., Chen, M., Sun, Y., Cao, B., Sun, J."HGF stimulates proliferation through the HGF/c-Met pathway in nasopharyngeal carcinoma cells". Oncology Letters 3.5 (2012): 1124-1128.
Chicago
Sun, R., Zhang, Q., Guo, L., Chen, M., Sun, Y., Cao, B., Sun, J."HGF stimulates proliferation through the HGF/c-Met pathway in nasopharyngeal carcinoma cells". Oncology Letters 3, no. 5 (2012): 1124-1128. https://doi.org/10.3892/ol.2012.613