Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53

  • Authors:
    • Katsuhiro Yasuda
    • Shunsuke Kato
    • Yasuhiro Sakamoto
    • Gou Watanabe
    • Satsuki Mashiko
    • Atsuko Sato
    • Yuichi Kakudo
    • Chikashi Ishioka
  • View Affiliations

  • Published online on: February 29, 2012     https://doi.org/10.3892/ol.2012.624
  • Pages: 978-982
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Abstract

After DNA damage, p53 is accumulated in the nucleus and transactivates downstream genes and induces apoptosis. There are two pathways in p53-dependent apoptosis, the transactivation-dependent and -independent pathway. In this study, we constructed p53-inducible glioblastoma cell lines and analyzed them for the induction of apoptosis and transactivation of p53-downstream genes after the nuclear or cytoplasmic expression of p53. To sequester p53 in the cytoplasm, we used p53 mutant with arginine to glycine substitution at residue 306 (R306G). Wild-type p53 retained the ability to arrest the cell cycle, and a p53 mutant with serine to phenylalanine substitution at residue 121 (S121F), which has a strong ability to induce apoptosis, retained this ability even when both the wild-type and p53 and S121F mutant were exclusively sequestered from the nucleus into the cytoplasm. Notably, cytoplasmically sequestered wild-type p53 and S121F mutant transactivated the downstream genes with distinct expression profiles, and the strong apoptotic ability of S121F was not associated with its transactivation activity. These results underscore the existence of transactivation-independent apoptosis and cytoplasmic function of p53.
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May 2012
Volume 3 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yasuda K, Kato S, Sakamoto Y, Watanabe G, Mashiko S, Sato A, Kakudo Y and Ishioka C: Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53. Oncol Lett 3: 978-982, 2012
APA
Yasuda, K., Kato, S., Sakamoto, Y., Watanabe, G., Mashiko, S., Sato, A. ... Ishioka, C. (2012). Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53. Oncology Letters, 3, 978-982. https://doi.org/10.3892/ol.2012.624
MLA
Yasuda, K., Kato, S., Sakamoto, Y., Watanabe, G., Mashiko, S., Sato, A., Kakudo, Y., Ishioka, C."Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53". Oncology Letters 3.5 (2012): 978-982.
Chicago
Yasuda, K., Kato, S., Sakamoto, Y., Watanabe, G., Mashiko, S., Sato, A., Kakudo, Y., Ishioka, C."Induction of apoptosis by cytoplasmically localized wild-type p53 and the S121F mutant super p53". Oncology Letters 3, no. 5 (2012): 978-982. https://doi.org/10.3892/ol.2012.624