Tanshinone IIA arrests cell cycle and induces apoptosis in 786-O human renal cell carcinoma cells

Wei,Xianli; Zhou,Lunbin; Hu,Liangyong; Huang,Yaoxiong

doi:10.3892/ol.2012.626

May 2012 Volume 3 Issue 5

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May 2012 Volume 3 Issue 5

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Article

Tanshinone IIA arrests cell cycle and induces apoptosis in 786-O human renal cell carcinoma cells

Authors:
- Xianli Wei
- Lunbin Zhou
- Liangyong Hu
- Yaoxiong Huang
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Affiliations: Department of Biomedical Engineering, Jinan University, Guangzhou, Guangdong 510632, P.R. China, Guangzhou Institute of Measuring and Testing Technology, Guangzhou, Guangdong 510030, P.R. China
Pages: 1144-1148
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Published online on: February 29, 2012

https://doi.org/10.3892/ol.2012.626
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Abstract

Tanshinone IIA (Tan IIA), isolated from the Chinese medicinal herb Danshen, has been reported to have anticancer effects in several tumor models, while its effects on renal cell carcinoma have not been studied. In the present study, we evaluated the effects of Tan IIA on growth inhibition and apoptosis in the renal cancer cell line 786-O and its mechanism of action. Results of the MTT assay indicated that the treatment of 786-O cells with Tan IIA resulted in a concentration-dependent decrease in cell viability. Flow cytometry analysis revealed that Tan IIA treatment caused apoptosis following cell cycle perturbation. Furthermore, we examined the expression of cell cycle and apoptosis-related proteins using immunoblotting, which indicated an upregulation of p53, p21, bax and caspase-3 in Tan IIA-treated cells compared with Tan IIA-untreated cells. These results suggest that the activation of p53 and the upregulation of its target genes, including p21 and bax, may be involved in the mitochondrial apoptosis induced by Tan IIA in 786-O cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Tanshinone IIA arrests cell cycle and induces apoptosis in 786-O human renal cell carcinoma cells

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