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Article

Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells

  • Authors:
    • Xia Ke
    • Ling Li
    • Hong-Lin Dong
    • Zhi-Nan Chen
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China, Cell Engineering Research Center and Department of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of General Surgery, Second Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China
  • Pages: 1249-1254
    |
    Published online on: March 27, 2012
       https://doi.org/10.3892/ol.2012.658
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Abstract

Acquisition of anoikis resistance is a prerequisite for the metastasis of hepatocellular carcinoma (HCC) cells. Activation of growth factor signaling pathways and rearrangement of the cytoskeleton have been reported as vital steps in this process. However, key molecules involved in anoikis resistance remain to be determined. The aim of this study was to investigate the effect of CD147 on HCC cells resistant to anoikis. The human SMMC-7221 human HCC cell line was used. Immunofluorescence was used to investigate the expression levels of CD147. Anoikis-induced cell death was assessed using trypan blue exclusion. In the present study, the results showed that SMMC-7721 HCC cells exhibited significant morphological changes when suspended in culture medium supplemented with 1% methocel and a subpopulation of cells resistant to anoikis was acquired with higher viability and invasion ability. CD147 was identified to be significantly increased in cells resistant to anoikis, when compared to the parental cells. CD147 knockdown by siRNA notably induced cell anoikis, partially through the inactivation of PI3K/Akt pathway. All of these evidence provide a novel CD147-related mechanism underlying the metastasis of HCC cells.
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1 

Simpson CD, Anyiwe K and Schimmer AD: Anoikis resistance and tumor metastasis. Cancer Lett. 272:177–185. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Frisch SM and Francis H: Disruption of epithelial cell-matrix interactions induces apoptosis. J Cell Biol. 124:619–626. 1994. View Article : Google Scholar : PubMed/NCBI

3 

Simpson CD, Mawji IA, Anyiwe K, et al: Inhibition of the sodium potassium adenosine triphosphatase pump sensitizes cancer cells to anoikis and prevents distant tumor formation. Cancer Res. 69:2739–2747. 2009. View Article : Google Scholar

4 

Taddei ML, Parri M, Angelucci A, et al: EphA2 induces metastatic growth regulating amoeboid motility and clonogenic potential in prostate carcinoma cells. Mol Cancer Res. 9:149–160. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Pongrakhananon V, Nimmannit U, Luanpitpong S, Rojanasakul Y and Chanvorachote P: Curcumin sensitizes non-small cell lung cancer cell anoikis through reactive oxygen species-mediated Bcl-2 downregulation. Apoptosis. 15:574–585. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Kumar P, Yadav A, Patel SN, et al: Tetrathiomolybdate inhibits head and neck cancer metastasis by decreasing tumor cell motility, invasiveness and by promoting tumor cell anoikis. Mol Cancer. 9:2062010. View Article : Google Scholar : PubMed/NCBI

7 

Aoudjit F and Vuori K: Matrix attachment regulates Fas-induced apoptosis in endothelial cells: a role for c-flip and implications for anoikis. J Cell Biol. 152:633–643. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Mawji IA, Simpson CD, Hurren R, et al: Critical role for Fas-associated death domain-like interleukin-1-converting enzyme-like inhibitory protein in anoikis resistance and distant tumor formation. J Natl Cancer Inst. 99:811–822. 2007. View Article : Google Scholar

9 

Smets FN, Chen Y, Wang LJ and Soriano HE: Loss of cell anchorage triggers apoptosis (anoikis) in primary mouse hepatocytes. Mol Genet Metab. 75:344–352. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Attwell S, Roskelley C and Dedhar S: The integrin-linked kinase (ILK) suppresses anoikis. Oncogene. 19:3811–3815. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Terada LS and Nwariaku FE: Escaping anoikis through ROS: ANGPTL4 controls integrin signaling through Nox1. Cancer Cell. 19:297–299. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Rosen K, Rak J, Leung T, Dean NM, Kerbel RS and Filmus J: Activated Ras prevents downregulation of Bcl-X(L) triggered by detachment from the extracellular matrix. A mechanism of Ras-induced resistance to anoikis in intestinal epithelial cells. J Cell Biol. 149:447–456. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Boisvert-Adamo K and Aplin AE: B-RAF and PI-3 kinase signaling protect melanoma cells from anoikis. Oncogene. 25:4848–4856. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Frisch SM, Vuori K, Ruoslahti E and Chan-Hui PY: Control of adhesion-dependent cell survival by focal adhesion kinase. J Cell Biol. 134:793–799. 1996. View Article : Google Scholar : PubMed/NCBI

15 

Zhao J, Zhang Y, Ithychanda SS, et al: Migfilin interacts with Src and contributes to cell-matrix adhesion-mediated survival signaling. J Biol Chem. 284:34308–34320. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Kang HG, Jenabi JM, Zhang J, et al: E-cadherin cell-cell adhesion in ewing tumor cells mediates suppression of anoikis through activation of the ErbB4 tyrosine kinase. Cancer Res. 67:3094–3105. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Caudroy S, Polette M, Tournier JM, et al: Expression of the extracellular matrix metalloproteinase inducer (EMMPRIN) and the matrix metalloproteinase-2 in bronchopulmonary and breast lesions. J Histochem Cytochem. 47:1575–1580. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Pinheiro C, Longatto-Filho A, Simoes K, et al: The prognostic value of CD147/EMMPRIN is associated with monocarboxylate transporter 1 co-expression in gastric cancer. Eur J Cancer. 45:2418–2424. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Guo H, Li R, Zucker S and Toole BP: EMMPRIN (CD147), an inducer of matrix metalloproteinase synthesis, also binds interstitial collagenase to the tumor cell surface. Cancer Res. 60:888–891. 2000.PubMed/NCBI

20 

Wu J, Ru NY, Zhang Y, et al: HAb18G/CD147 promotes epithelial-mesenchymal transition through TGF-beta signaling and is transcriptionally regulated by Slug. Oncogene. 30:4410–4427. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Yang JM, O’Neill P, Jin W, et al: Extracellular matrix metalloproteinase inducer (CD147) confers resistance of breast cancer cells to Anoikis through inhibition of Bim. J Biol Chem. 281:9719–9727. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Xu J, Shen ZY, Chen XG, et al: A randomized controlled trial of Licartin for preventing hepatoma recurrence after liver transplantation. Hepatology. 45:269–276. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Ma XK, Wang L, Li Y, et al: HAb18G/CD147 cell-cell contacts confer resistance of a HEK293 subpopulation to anoikis in an E-cadherin-dependent manner. BMC Cell Biol. 11:272010. View Article : Google Scholar : PubMed/NCBI

24 

Frisch SM: Evidence for a function of death-receptor-related, death-domain-containing proteins in anoikis. Curr Biol. 9:1047–1049. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Chen ZN: Significance and application of anti-malignant hepatoma MAb HAb18 in radioimmunal diagnosis of human hepatocellular carcinoma. Zhonghua Zhong Liu Za Zhi. 14:9–12. 1992.(In Chinese).

26 

Gou X, Ru Q, Zhang H, et al: HAb18G/CD147 inhibits starvation-induced autophagy in human hepatoma cell SMMC7721 with an involvement of Beclin 1 down-regulation. Cancer Sci. 100:837–843. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Xu J, Xu HY, Zhang Q, et al: HAb18G/CD147 functions in invasion and metastasis of hepatocellular carcinoma. Mol Cancer Res. 5:605–614. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Derksen PW, Braumuller TM, van der Burg E, et al: Mammary-specific inactivation of E-cadherin and p53 impairs functional gland development and leads to pleomorphic invasive lobular carcinoma in mice. Dis Model Mech. 4:347–358. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Singh AB, Sugimoto K and Harris RC: Juxtacrine activation of epidermal growth factor (EGF) receptor by membrane-anchored heparin-binding EGF-like growth factor protects epithelial cells from anoikis while maintaining an epithelial phenotype. J Biol Chem. 282:32890–32901. 2007. View Article : Google Scholar

30 

Wu Y, Zuo J, Ji G, et al: Proapoptotic function of integrin beta(3) in human hepatocellular carcinoma cells. Clin Cancer Res. 15:60–69. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Feng Y, Wang LY, Cai T, et al: All-trans-retinoic acid increased the expression of integrin alpha5beta1 and induced ‘anoikis’ in SMMC-7721 hepatocarcinoma cell. J Exp Clin Cancer Res. 20:429–438. 2001.PubMed/NCBI

32 

McFall A, Ulku A, Lambert QT, Kusa A, Rogers-Graham K and Der CJ: Oncogenic Ras blocks anoikis by activation of a novel effector pathway independent of phosphatidylinositol 3-kinase. Mol Cell Biol. 21:5488–5499. 2001. View Article : Google Scholar : PubMed/NCBI

33 

Isakoff SJ, Engelman JA, Irie HY, et al: Breast cancer-associated PIK3CA mutations are oncogenic in mammary epithelial cells. Cancer Res. 65:10992–11000. 2005. View Article : Google Scholar : PubMed/NCBI

34 

Jiang K, Sun J, Cheng J, Djeu JY, Wei S and Sebti S: Akt mediates Ras downregulation of RhoB, a suppressor of transformation, invasion, and metastasis. Mol Cell Biol. 24:5565–5576. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Frisch SM and Ruoslahti E: Integrins and anoikis. Curr Opin Cell Biol. 9:701–706. 1997. View Article : Google Scholar : PubMed/NCBI

36 

Chen Y, Zhang H, Gou X, Horikawa Y, Xing J and Chen Z: Upregulation of HAb18G/CD147 in activated human umbilical vein endothelial cells enhances the angiogenesis. Cancer Lett. 278:113–121. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ke X, Li L, Dong H and Chen Z: Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells. Oncol Lett 3: 1249-1254, 2012.
APA
Ke, X., Li, L., Dong, H., & Chen, Z. (2012). Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells. Oncology Letters, 3, 1249-1254. https://doi.org/10.3892/ol.2012.658
MLA
Ke, X., Li, L., Dong, H., Chen, Z."Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells". Oncology Letters 3.6 (2012): 1249-1254.
Chicago
Ke, X., Li, L., Dong, H., Chen, Z."Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells". Oncology Letters 3, no. 6 (2012): 1249-1254. https://doi.org/10.3892/ol.2012.658
Copy and paste a formatted citation
x
Spandidos Publications style
Ke X, Li L, Dong H and Chen Z: Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells. Oncol Lett 3: 1249-1254, 2012.
APA
Ke, X., Li, L., Dong, H., & Chen, Z. (2012). Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells. Oncology Letters, 3, 1249-1254. https://doi.org/10.3892/ol.2012.658
MLA
Ke, X., Li, L., Dong, H., Chen, Z."Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells". Oncology Letters 3.6 (2012): 1249-1254.
Chicago
Ke, X., Li, L., Dong, H., Chen, Z."Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells". Oncology Letters 3, no. 6 (2012): 1249-1254. https://doi.org/10.3892/ol.2012.658
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