Analysis and results of Ku and XRCC4 expression in hypopharyngeal cancer tissues treated with chemoradiotherapy

Hayashi,Jyunichi; Sakata,Koh-Ichi; Someya,Masanori; Matsumoto,Yoshihisa; Satoh,Masaaki; Nakata,Kensei; Hori,Masakazu; Takagi,Masaru; Kondoh,Atsushi; Himi,Tetsuo; Hareyama,Masato

doi:10.3892/ol.2012.674

July 2012 Volume 4 Issue 1

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July 2012 Volume 4 Issue 1

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Article

Analysis and results of Ku and XRCC4 expression in hypopharyngeal cancer tissues treated with chemoradiotherapy

Authors:
- Jyunichi Hayashi
- Koh-Ichi Sakata
- Masanori Someya
- Yoshihisa Matsumoto
- Masaaki Satoh
- Kensei Nakata
- Masakazu Hori
- Masaru Takagi
- Atsushi Kondoh
- Tetsuo Himi
- Masato Hareyama
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Affiliations: Department of Radiology, Sapporo Medical University, School of Medicine, Hokkaido, Japan, Tokyo Institute of Technology, Research Laboratory for Nuclear Reactors, Tokyo, Japan, Division of Clinical Pathology, NTT Sapporo Hospital, Sapporo, Japan, Department of Otolaryngology, Sapporo Medical University, School of Medicine, Hokkaido, Japan
Pages: 151-155
|
Published online on: April 5, 2012

https://doi.org/10.3892/ol.2012.674
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Abstract

DNA double-strand break (DSB) is one of the most serious forms of damage induced by ionizing irradiation. Non-homologous end-joining (NHEJ) is a key mechanism of DNA DSB repair. The immunohistochemical analysis of proteins involved in NHEJ may have potential as a predictive assay for tumor radiosensitivity. We examined the correlation between the expression of proteins involved in DNA DSB in biopsy specimens and the results of chemoradiotherapy in hypopharyngeal cancers. Fifty-seven patients with previously untreated squamous cell carcinoma of the hypopharynx were treated between March 2002 and December 2009. Most patients (75%) had stage III or IV disease. The chemotherapy consisted of cisplatin plus 5FU or S-1. A tumor dose of 50 Gy was usually administered to the primary tumor and regional lymph nodes. Doses of 10-20 Gy were usually added to the primary tumor with reduced fields after 50 Gy. The 5-year disease-free survival rate was 100% for patients in stage I, 90% in stage II, 64% in stage III and 50% in stage IV. In stages I-III, patients with a lower expression of Ku70 or XRCC4 tended to have better locoregional control. These results indicated that a lower expression of Ku70 or XRCC4 may be correlated with higher radiosensitivity. Two patients had distant metastasis alone, of which one had 0% expression of Ku70 and the other had 0% expression of Ku86. The absence of Ku70 or Ku86 expression indicates low DNA-PK activity. Low DNA-PK activity due to a low expression of Ku may result in the genetic alteration of cancer cells, leading to a higher tendency of distant metastasis. This finding suggests that proteins involved in NHEJ may have an impact on the treatment results of chemoradiotherapy in hypopharyngeal cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Analysis and results of Ku and XRCC4 expression in hypopharyngeal cancer tissues treated with chemoradiotherapy

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