Effect of RNA interference-related HiWi gene expression on the proliferation and apoptosis of lung cancer stem cells

Liang,Dong; Fang,Zehui; Dong,Min; Liang,Chao; Xing,Chonghao; Zhao,Juan; Yang,Yiju

doi:10.3892/ol.2012.677

July 2012 Volume 4 Issue 1

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July 2012 Volume 4 Issue 1

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Article

Effect of RNA interference-related HiWi gene expression on the proliferation and apoptosis of lung cancer stem cells

Authors:
- Dong Liang
- Zehui Fang
- Min Dong
- Chao Liang
- Chonghao Xing
- Juan Zhao
- Yiju Yang
View Affiliations / Copyright

Affiliations: Hainan Province Nongken Sanya Hospital, Sanya, Hainan 572023, P.R. China, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, The First Afﬁliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
Pages: 146-150
|
Published online on: April 11, 2012

https://doi.org/10.3892/ol.2012.677
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Abstract

The aim of this study was to investigate the effect of HiWi gene silencing on lung cancer tumor stem cell proliferation and apoptosis using gene transfection and RNA interference. Moreover, we examined the feasibility of using the HiWi gene as a molecular target for the inhibition of lung cancer tumor stem cells (TSCs). shRNA eukaryotic expression vectors, pGenesil-2-HiWi1, pGenesil‑2-HiWi2263 and pGenesil-2-control, targeting the HiWi gene were constructed. PBS served as the control group. The expression vector of the target HiWi gene shRNA was transfected into lung cancer TSCs with PEI as the medium. The conditions of lung cancer TSC proliferation and apoptosis in each group were examined using an MTT assay, fluorescence‑activated cell sorting and Annexin V staining. The results showed that 24 h after transfection, the proliferation inhibition rates in the pGenesil-2-HiWi2263 (81.62%) and pGenesil‑2-HiWi1 (73.16%) groups were higher as compared to the proliferation inhibition rate in the pGenesil-2-control group (8.54%). The apoptotic ratios in the pGenesil-2-HiWi1 and pGenesil-2-HiWi2263 groups were 26.16±1.21 and 28.06±1.78%, respectively, were higher as compared to those in the pGenesil‑2-control group 2.86±0.09% (P<0.01). Our results suggest that HiWi gene silencing decreases proliferation and promotes apoptosis of lung cancer TSCs. Therefore, the HiWi gene could be used as a molecular target for the inhibition of the growth of lung cancer TSCs, which has potential value for the treatment of lung cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of RNA interference-related HiWi gene expression on the proliferation and apoptosis of lung cancer stem cells

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