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Article

Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells

  • Authors:
    • Yuting Yao
    • Lu Li
    • He Zhang
    • Renbing Jia
    • Bo Liu
    • Xiaoping Zhao
    • Leilei Zhang
    • Guanxiang Qian
    • Xianqun Fan
    • Shengfang Ge
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Department of Biochemistry and Molecular Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China
  • Pages: 163-167
    |
    Published online on: April 17, 2012
       https://doi.org/10.3892/ol.2012.682
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Abstract

Vitamin K2 (VK2) exerts cell growth inhibitory effects in various human cancer cells such as SMMC-7721 hepatocellular carcinoma (HCC) cells. BCL-2 is an antiapoptotic protein that is frequently overexpressed in numerous tumors. Modulation of multiple antiapoptotic signaling pathways involving BCL-2, which are related to growth factor-stimulated signal transduction in cell survival, is essential for enhancement of the cytotoxic effect of anticancer drugs. In this study, we tested a new strategy of gene therapy by combining BCL-2 siRNA with VK2. In SMMC-7721 HCC cells, the combined treatment significantly enhanced cytotoxicity compared with treatment with either VK2 or siBCL-2 alone. We found that combined treatment induced a significantly different level of G2 stage inhibition. Furthermore, the p53 protein was overexpressed 24 h subsequent to combination treatment, and p21 was clearly increased at 36 h as a consequence of the increased p53 activity. In conclusion, these data suggest that the antitumor effect of VK2 may be improved by silencing BCL-2 expression in SMMC-7721 HCC cells and provides support for the combined use of VK2 and siBCL-2 as a promising approach in cancer gene therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Yao Y, Li L, Zhang H, Jia R, Liu B, Zhao X, Zhang L, Qian G, Fan X, Ge S, Ge S, et al: Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells. Oncol Lett 4: 163-167, 2012.
APA
Yao, Y., Li, L., Zhang, H., Jia, R., Liu, B., Zhao, X. ... Ge, S. (2012). Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells. Oncology Letters, 4, 163-167. https://doi.org/10.3892/ol.2012.682
MLA
Yao, Y., Li, L., Zhang, H., Jia, R., Liu, B., Zhao, X., Zhang, L., Qian, G., Fan, X., Ge, S."Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells". Oncology Letters 4.1 (2012): 163-167.
Chicago
Yao, Y., Li, L., Zhang, H., Jia, R., Liu, B., Zhao, X., Zhang, L., Qian, G., Fan, X., Ge, S."Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells". Oncology Letters 4, no. 1 (2012): 163-167. https://doi.org/10.3892/ol.2012.682
Copy and paste a formatted citation
x
Spandidos Publications style
Yao Y, Li L, Zhang H, Jia R, Liu B, Zhao X, Zhang L, Qian G, Fan X, Ge S, Ge S, et al: Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells. Oncol Lett 4: 163-167, 2012.
APA
Yao, Y., Li, L., Zhang, H., Jia, R., Liu, B., Zhao, X. ... Ge, S. (2012). Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells. Oncology Letters, 4, 163-167. https://doi.org/10.3892/ol.2012.682
MLA
Yao, Y., Li, L., Zhang, H., Jia, R., Liu, B., Zhao, X., Zhang, L., Qian, G., Fan, X., Ge, S."Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells". Oncology Letters 4.1 (2012): 163-167.
Chicago
Yao, Y., Li, L., Zhang, H., Jia, R., Liu, B., Zhao, X., Zhang, L., Qian, G., Fan, X., Ge, S."Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells". Oncology Letters 4, no. 1 (2012): 163-167. https://doi.org/10.3892/ol.2012.682
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