Methylation and aberrant expression of the Wnt antagonist secreted Frizzled-related protein 1 in bladder cancer

Wang,Xiaobin; Wang,Huihan; Bu,Renge; Fei,Xiang; Zhao,Chenghai; Song,Yongsheng

doi:10.3892/ol.2012.713

August 2012 Volume 4 Issue 2

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August 2012 Volume 4 Issue 2

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Article

Methylation and aberrant expression of the Wnt antagonist secreted Frizzled-related protein 1 in bladder cancer

Authors:
- Xiaobin Wang
- Huihan Wang
- Renge Bu
- Xiang Fei
- Chenghai Zhao
- Yongsheng Song
View Affiliations / Copyright

Affiliations: Department of Urology, Shengjing Hospital affiliated to China Medical University, Shenyang 110004, P.R. China, Department of Hematology Shengjing Hospital affiliated to China Medical University, Shenyang 110004, P.R. China, Department of Pathophysiology, Shengjing Hospital affiliated to China Medical University, Shenyang 110004, P.R. China
Pages: 334-338
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Published online on: May 11, 2012

https://doi.org/10.3892/ol.2012.713
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Abstract

The aims of this study were to determine the methylation and expression status of secreted Frizzled-related protein 1 (SFRP1) in bladder cancer, to explore the mechanisms involved and to study the role of SFRP1 in the pathogenesis of bladder cancer. SFRP1 mRNA was detected by reverse transcription PCR (RT-PCR). The DNA methylation status was determined by methylation-specific PCR and protein was detected using western blotting. The results of the present study demonstrated that SFRP1 was methylated in the bladder cancer cell lines T24 and 5637, but not in SCaBER cells. After treating T24 and 5637 cells with a demethylating agent, the cells expressed SFRP1 mRNA and protein. Among the 45 patients with bladder cancer, methylation of SFRP1 was detected in 28 patients (62.2%). Of the matched cancer-adjacent tissues, 6 (13.3%) were found to have methylated SFRP1. The result is statistically significant (P<0.01). In conclusion, SFRP1 is downregulated in certain bladder cancer patients as a consequence of methylation. SFRP1 methylation may be involved in the pathogenesis of bladder cancer via excessive activation of the Wnt signaling pathway.

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1	Katanaev VL: The Wnt/Frizzled GPCR signaling pathway. Biochemistry (Mosc). 75:1428–1434. 2010. View Article : Google Scholar : PubMed/NCBI
2	Esteve P and Bovolenta P: The advantages and disadvantages of sfrp1 and sfrp2 expression in pathological events. Tohoku J Exp Med. 221:11–17. 2010. View Article : Google Scholar : PubMed/NCBI
3	Zou H, Molina JR, Harrington JJ, et al: Aberrant methylation of secreted frizzled-related protein genes in esophageal adenocarcinoma and Barrett’s esophagus. Int J Cancer. 116:584–591. 2005.PubMed/NCBI
4	Lodygin D, Epanchintsev A, Menssen A, Diebold J and Hermeking H: Functional epigenomics identifies genes frequently silenced in prostate cancer. Cancer Res. 65:4218–4227. 2005. View Article : Google Scholar : PubMed/NCBI
5	Bui TD, O’Brien T, Crew J, Cranston D and Harris AL: High expression of Wnt7b in human superficial bladder cancer vs invasive bladder cancer. Br J Cancer. 77:319–324. 1998. View Article : Google Scholar : PubMed/NCBI
6	Zhuang L, Zhang Z and Guo Y: Aberrant expression of growth factor Wnt-5A in six urinary malignant cell lines. Chin Med J (Engl). 112:251–255. 1999.PubMed/NCBI
7	Urakami S, Shiina H, Enokida H, et al: Epigenetic inactivation of Wnt inhibitory factor-1 plays an important role in bladder cancer through aberrant canonical Wnt/beta-catenin signaling pathway. Clin Cancer Res. 12:383–391. 2006. View Article : Google Scholar : PubMed/NCBI
8	Tycko B: Cancer epigenetics and targeted therapies. Oncology (Williston Park) 25. 228:2312011.PubMed/NCBI
9	Ezan J, Leroux L, Barandon L, et al: FrzA/sFRP-1, a secreted antagonist of the Wnt-Frizzled pathway, controls vascular cell proliferation in vitro and in vivo. Cardiovasc Res. 63:731–738. 2004. View Article : Google Scholar : PubMed/NCBI
10	Häusler KD, Horwood NJ, Chuman Y, et al: Secreted frizzled-related protein-1 inhibits RANKL-dependent osteoclast formation. J Bone Miner Res. 19:1873–1881. 2004.PubMed/NCBI
11	Bodine PV, Zhao W, Kharode YP, et al: The Wnt antagonist secreted frizzled-related protein-1 is a negative regulator of trabecular bone formation in adult mice. Mol Endocrinol. 18:1222–1237. 2004. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Methylation and aberrant expression of the Wnt antagonist secreted Frizzled-related protein 1 in bladder cancer

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