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Article

Bisphosphonates regulate cell proliferation, apoptosis and pro-osteoclastic expression in MG‑63 human osteosarcoma cells

  • Authors:
    • Jun Chang
    • Wei Wang
    • Hui Zhang
    • Yong Hu
    • Zongsheng Yin
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, First Affiliated Hospital, Anhui Medical University, Hefei, Anhui 230022, P.R. China
  • Pages: 299-304
    |
    Published online on: May 21, 2012
       https://doi.org/10.3892/ol.2012.723
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Abstract

Bisphosphonates are well established in the management of cancer-induced skeletal complications. Recent studies suggest that nitrogen-containing bisphosphonates (N-BPs) promote the apoptosis of cancer cells as well as osteoclasts in bone metastatic sites. To investigate whether N-BPs exhibit a direct antitumor effect on osteoclasts, the current study investigated the effects of zoledronic acid (ZOL) on MG-63 cells in vitro. MG-63 cells were treated with ZOL. The inhibitory effect of ZOL on the growth of MG-63 cells was measured by MTT assay. ZOL-induced apoptosis of the MG-63 cells was examined by Hoechst 33258 staining, electron microscopy, Annexin V-FITC and propidium iodide staining. Reverse-transcription polymerase chain reaction (RT-PCR) and western blotting analysis were employed to assess the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL). The MTT assay showed that ZOL induced a distinct dose- and time‑dependent reduction of cell viability with an IC50 value of 52.37±1.0 µM for 72 h. Flow cytometric analysis further revealed that the cell apoptosis was induced by arrest of the cell cycle in the G1 phase. RT-PCR and western blot analysis demonstrated that ZOL upregulated OPG expression. These results suggest that ZOL has direct effects on osteosarcoma cell growth and apoptosis. Increased OPG expression is an indirect effect, possibly via changes in the local microenvironment.
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Copy and paste a formatted citation
Spandidos Publications style
Chang J, Wang W, Zhang H, Hu Y and Yin Z: Bisphosphonates regulate cell proliferation, apoptosis and pro-osteoclastic expression in MG‑63 human osteosarcoma cells. Oncol Lett 4: 299-304, 2012.
APA
Chang, J., Wang, W., Zhang, H., Hu, Y., & Yin, Z. (2012). Bisphosphonates regulate cell proliferation, apoptosis and pro-osteoclastic expression in MG‑63 human osteosarcoma cells. Oncology Letters, 4, 299-304. https://doi.org/10.3892/ol.2012.723
MLA
Chang, J., Wang, W., Zhang, H., Hu, Y., Yin, Z."Bisphosphonates regulate cell proliferation, apoptosis and pro-osteoclastic expression in MG‑63 human osteosarcoma cells". Oncology Letters 4.2 (2012): 299-304.
Chicago
Chang, J., Wang, W., Zhang, H., Hu, Y., Yin, Z."Bisphosphonates regulate cell proliferation, apoptosis and pro-osteoclastic expression in MG‑63 human osteosarcoma cells". Oncology Letters 4, no. 2 (2012): 299-304. https://doi.org/10.3892/ol.2012.723
Copy and paste a formatted citation
x
Spandidos Publications style
Chang J, Wang W, Zhang H, Hu Y and Yin Z: Bisphosphonates regulate cell proliferation, apoptosis and pro-osteoclastic expression in MG‑63 human osteosarcoma cells. Oncol Lett 4: 299-304, 2012.
APA
Chang, J., Wang, W., Zhang, H., Hu, Y., & Yin, Z. (2012). Bisphosphonates regulate cell proliferation, apoptosis and pro-osteoclastic expression in MG‑63 human osteosarcoma cells. Oncology Letters, 4, 299-304. https://doi.org/10.3892/ol.2012.723
MLA
Chang, J., Wang, W., Zhang, H., Hu, Y., Yin, Z."Bisphosphonates regulate cell proliferation, apoptosis and pro-osteoclastic expression in MG‑63 human osteosarcoma cells". Oncology Letters 4.2 (2012): 299-304.
Chicago
Chang, J., Wang, W., Zhang, H., Hu, Y., Yin, Z."Bisphosphonates regulate cell proliferation, apoptosis and pro-osteoclastic expression in MG‑63 human osteosarcoma cells". Oncology Letters 4, no. 2 (2012): 299-304. https://doi.org/10.3892/ol.2012.723
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