Evaluation of the uptake function of liver in rats with obstructive jaundice before and after relief from obstruction by superparamagnetic iron oxide‑enhanced magnetic resonance imaging

Yiming,Zhou; Lei,Jiang; Renyou,Zhai; Shan,Ke; Xue,Li; Min,Liu; Jun,Gao

doi:10.3892/ol.2012.729

August 2012 Volume 4 Issue 2

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August 2012 Volume 4 Issue 2

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Article

Evaluation of the uptake function of liver in rats with obstructive jaundice before and after relief from obstruction by superparamagnetic iron oxide‑enhanced magnetic resonance imaging

Authors:
- Zhou Yiming
- Jiang Lei
- Zhai Renyou
- Ke Shan
- Li Xue
- Liu Min
- Gao Jun
View Affiliations / Copyright

Affiliations: Department of Radiology, Beijing Chao-Yang Hospital, Capital Medical University, Chaoyang District, Beijing 100020, P.R. China, Department of Radiology, Beijing Hospital, Dongcheng District, Beijing 100730, P.R. China, Department of Hepatobiliary Surgery, Beijing Chao-Yang Hospital (Jingxi Campus), Capital Medical University, Shijingshan District, Beijing 100043, P.R. China, Department of Pathology, Beijing Chao-Yang Hospital, Capital Medical University, Chaoyang District, Beijing 100020, P.R. China
Pages: 221-226
|
Published online on: May 24, 2012

https://doi.org/10.3892/ol.2012.729
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Abstract

The aim of this study was to investigate the changes in the uptake function of the liver in rats with obstructive jaundice prior to and following relief from obstructive jaundice, and to investigate whether superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance imaging (MRI) could be used to assess liver uptake function. In total, 40 male Sprague Dawley rats were randomly assigned into four groups: Obstructive jaundice, internal drainage, external drainage and sham surgery. The common bile ducts in the obstructive jaundice group were ligated. Internal drainage (ID) and external drainage (ED) groups were ligated, followed by internal drainage or external drainage, respectively. The T2 and T2* values of the liver parenchyma were measured. Liver sections were stained with Perls' Prussian blue, and hematoxylin and eosin. The number of SPIO-nanoparticle clusters was counted manually using a microscope. Total bilirubin of the blood was measured. Results showed that the T2 and T2* values and total bilirubin of the obstructive jaundice group were significantly higher compared to the other three groups. The number of SPIO-nanoparticle clusters in the obstructive jaundice group was significantly lower compared to the other three groups. In conclusion, obstructive jaundice suppresses liver uptake function in rats, which may be reversed by internal and external biliary drainage. However, no significant difference was found between the therapeutic effect of ID and ED on liver uptake function. Thus, SPIO-enhanced MRI may be used to evaluate the uptake function of the liver.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Evaluation of the uptake function of liver in rats with obstructive jaundice before and after relief from obstruction by superparamagnetic iron oxide‑enhanced magnetic resonance imaging

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