Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5

Jiang,Xiu  Xiu; Xu,Kai  Hong; Ma,Jun  Yan; Tian,Yong  Hong; Guo,Xiao  Yan; Lin,Jun; Wu,Rui  Jin

doi:10.3892/ol.2012.738

Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5

Authors:
- Xiu Xiu Jiang
- Kai Hong Xu
- Jun Yan Ma
- Yong Hong Tian
- Xiao Yan Guo
- Jun Lin
- Rui Jin Wu
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Affiliations: Department of Gynecology and Obstetrics, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China, Laboratory of Gynecology and Obstetrics, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China
Published online on: May 30, 2012 https://doi.org/10.3892/ol.2012.738
Pages: 257-261

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Abstract

Aquaporin (AQP)-dependent cell migration has broad implications in angiogenesis, tumor metastasis, wound healing, glial scarring and other events requiring cell movement. There are 13 isoforms of AQP (0-12) that have been identified in mammals. It is unclear whether AQP5 plays a role in the development of endometrial cancer. We recently demonstrated that ovarian steroids may affect the expression of AQP5 in the female genital tract. In this study, we considered whether AQP5 may affect cell migration in Ishikawa cells, an adenocarcinoma cell line derived from the endometrium. The results showed that the downregulation of AQP5 results in reduced Ishikawa cell migration. The estrogen (E2) receptor in the promoter of AQP5 mediated the regulation of AQP5 expression in the normal endometrium and endometrial cancer. By contrast, the upregulation of AQP5 by E2 increased cell migration, invasion and adhesion through increased annexin-2, which is responsible for F-actin remodeling and rearrangement. E2 regulates Ishikawa cell migration by regulating the AQP5 expression.

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