IL-23 directly enhances the proliferative and invasive activities of colorectal carcinoma

Suzuki,Hideyuki; Ogawa,Hitoshi; Miura,Koh; Haneda,Sho; Watanabe,Kazuhiro; Ohnuma,Shinobu; Sasaki,Hiroyuki; Sase,Tomohiko; Kimura,Shunichi; Kajiwara,Taiki; Komura,Toshihiro; Toshima,Masahide; Matsuda,Yasufumi; Shibata,Chikashi; Sasaki,Iwao

doi:10.3892/ol.2012.739

August 2012 Volume 4 Issue 2

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August 2012 Volume 4 Issue 2

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Article

IL-23 directly enhances the proliferative and invasive activities of colorectal carcinoma

Authors:
- Hideyuki Suzuki
- Hitoshi Ogawa
- Koh Miura
- Sho Haneda
- Kazuhiro Watanabe
- Shinobu Ohnuma
- Hiroyuki Sasaki
- Tomohiko Sase
- Shunichi Kimura
- Taiki Kajiwara
- Toshihiro Komura
- Masahide Toshima
- Yasufumi Matsuda
- Chikashi Shibata
- Iwao Sasaki
View Affiliations / Copyright

Affiliations: Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine, Sendai 980-88574, Japan
Pages: 199-204
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Published online on: May 30, 2012

https://doi.org/10.3892/ol.2012.739
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Abstract

Interleukin-23 (IL-23) plays an essential role in the mucosal immune system. It has been suggested that IL-23 is able to induce carcinogenesis as well as inflammation and a recent study revealed that IL-23R is expressed in colorectal carcinoma cells. However, neither the differences in the IL-23R expression among the patients nor the concrete functions of IL-23 in colorectal carcinoma cells have been revealed. The aim of the present study was to examine the characteristics of IL-23R expression in colorectal carcinoma and the direct effects of IL-23 on colorectal cancer cells. We examined the IL-23R expression in human colorectal cancer tissue samples by immunohistochemistry. Cell proliferation and invasion assays under IL-23 stimulation were performed using cultured cells derived from colorectal cancer. ELISA and real-time PCR were used to evaluate the transforming growth factor (TGF)-β production due to IL-23 stimulation. All of the TNM stage IV patients were positive for IL-23R. IL-23R expression in the carcinoma tissue was also relatively high at the deepest point of invasion in certain cases. The proliferative and invasive activities and/or TGF-β production of DLD-1 cells increased by IL-23 stimulation, whereas no change was observed in the activities of MIP101 and KM12c cells. IL-23 directly enhanced the malignancy of the colon carcinoma cells. An autocrine mechanism via TGF-β production may underlie these effects. IL-23 is therefore a potential target for cancer immunotherapy. However, the homogeneity in IL-23R expression and the effects of IL-23 on colorectal carcinoma cells should be considered.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

IL-23 directly enhances the proliferative and invasive activities of colorectal carcinoma

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