Mesenchymal stem cell-like cells in classic renal angiomyolipoma

Yan,Xinlong; Shi,Lixin; Chen,Guangfu; Zhang,Xu; Liu,Bing; Yue,Wen; Pei,Xuetao; Sun,Shengkun

doi:10.3892/ol.2012.760

Mesenchymal stem cell-like cells in classic renal angiomyolipoma

Authors:
- Xinlong Yan
- Lixin Shi
- Guangfu Chen
- Xu Zhang
- Bing Liu
- Wen Yue
- Xuetao Pei
- Shengkun Sun
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Affiliations: Stem Cells and Regenerative Medicine Laboratory, Institute of Beijing Transfusion Medicine, Beijing 100850, P.R. China , Department of Urology, PLA General Hospital, Beijing 100853, P.R. China, Department of Tumor Molecular Biology, Affiliated Hospital to the Academy of Medical Sciences, Beijing 100071, P.R. China
Published online on: June 14, 2012 https://doi.org/10.3892/ol.2012.760
Pages: 398-402

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Abstract

As a benign mesenchymal tumor, classic renal angiomyolipoma (AML) may obliterate the kidney parenchyma and cause renal hemorrhage. It has previously been reported that mesenchymal stem cells (MSCs) are involved in tumorigenesis; however, there have been no studies on stem cells with renal AML origin. In the present study, six females with classic renal AML received a partial or total nephrectomy. During surgery, tumor tissues were collected and culture expansion of adhesive fibroblastoid cells from these tissues was performed. We successfully isolated and cultured MSC‑like cells from all six renal AML tumors. MSC characteristics, including morphology, immunophenotype and multidifferentiation potential were analyzed. Flow cytometry analysis revealed that these cells are highly similar to human bone marrow MSCs due to the expression of MSC‑speciﬁc surface proteins, including CD29, CD44, CD73, CD90 and CD105. The stem cell‑like nature of these cells is further supported by their adipogenic and osteogenic differentiation potentials when incubated in appropriate differentiation cocktails. Renal AML‑derived adhesive cells possessing the characteristics of MSCs are described for the first time. They are a novel cell type which may be useful in future studies with regards to determining the role of stem cells in the formation and development of renal AML.

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1	Lim SD, Stallcup W, Lefkove B, Govindarajan B, Au KS, Northrup H, et al: Expression of the neural stem cell markers NG2 and L1 in human angiomyolipoma: are angiomyolipomas neoplasms of stem cells? Mol Med. 13:160–165. 2007.PubMed/NCBI
2	Bonetti F, Pea M, Martignoni G, Doglioni C, Zamboni G, Capelli P, et al: Clear cell (‘sugar’) tumor of the lung is a lesion strictly related to angiomyolipoma - the concept of a family of lesions characterized by the presence of the perivascular epithelioid cells (PEC). Pathology. 26:230–236. 1994.
3	Barnard M and Lajoie G: Angiomyolipoma: immunohistochemical and ultrastructural study of 14 cases. Ultrastruct Pathol. 25:21–29. 2001. View Article : Google Scholar : PubMed/NCBI
4	Cao H, Xu W, Qian H, Zhu W, Yan Y, Zhou H, et al: Mesenchymal stem cell-like cells derived from human gastric cancer tissues. Cancer Lett. 274:61–71. 2009. View Article : Google Scholar : PubMed/NCBI
5	Bussolati B, Bruno S, Grange C, Ferrando U and Camussi G: Identification of a tumor-initiating stem cell population in human renal carcinomas. FASEB J. 22:3696–3705. 2008. View Article : Google Scholar : PubMed/NCBI
6	Gupta S, Verfaillie C, Chmielewski D, Kren S, Eidman K, Connaire J, et al: Isolation and characterization of kidney-derived stem cells. J Am Soc Nephrol. 17:3028–3040. 2006. View Article : Google Scholar : PubMed/NCBI
7	da Silva Meirelles L, Chagastelles PC and Nardi NB: Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J Cell Sci. 119:2204–2213. 2006.PubMed/NCBI
8	Plotkin MD and Goligorsky MS: Mesenchymal cells from adult kidney support angiogenesis and differentiate into multiple interstitial cell types including erythropoietin-producing fibroblasts. Am J Physiol Renal Physiol. 291:F902–F912. 2006. View Article : Google Scholar
9	Humphreys BD and Bonventre JV: Mesenchymal stem cells in acute kidney injury. Annu Rev Med. 59:311–325. 2008. View Article : Google Scholar : PubMed/NCBI
10	Ooi SM, Vivian JB and Cohen RJ: The use of the Ki-67 marker in the pathological diagnosis of the epithelioid variant of renal angiomyolipoma. Int Urol Nephrol. 41:559–565. 2009. View Article : Google Scholar : PubMed/NCBI
11	Sun S, Guo Z, Xiao X, Liu B, Liu X, Tang PH, et al: Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method. Stem Cells. 21:527–535. 2003. View Article : Google Scholar : PubMed/NCBI
12	Aractingi S, Kanitakis J, Euvrard S, Le Danff C, Peguillet I, Khosrotehrani K, et al: Skin carcinoma arising from donor cells in a kidney transplant recipient. Cancer Res. 65:1755–1760. 2005. View Article : Google Scholar : PubMed/NCBI
13	Barozzi P, Luppi M, Facchetti F, Mecucci C, Alù M, Sarid R, et al: Post-transplant Kaposi sarcoma originates from the seeding of donor-derived progenitors. Nat Med. 9:554–561. 2003. View Article : Google Scholar : PubMed/NCBI
14	Houghton J, Stoicov C, Nomura S, Rogers AB, Carlson J, Li H, et al: Gastric cancer originating from bone marrow-derived cells. Science. 306:1568–1571. 2004. View Article : Google Scholar : PubMed/NCBI
15	Li H, Fan X, Kovi RC, Jo Y, Moquin B, Konz R, et al: Spontaneous expression of embryonic factors and p53 point mutations in aged mesenchymal stem cells: a model of age-related tumorigenesis in mice. Cancer Res. 67:10889–10898. 2007. View Article : Google Scholar : PubMed/NCBI
16	Tirode F, Laud-Duval K, Prieur A, Delorme B, Charbord P and Delattre O: Mesenchymal stem cell features of Ewing tumors. Cancer Cell. 11:421–429. 2007. View Article : Google Scholar : PubMed/NCBI
17	Royer-Pokora B, Busch M, Beier M, Duhme C, de Torres C, Mora J, et al: Wilms tumor cells with WT1 mutations have characteristic features of mesenchymal stem cells and express molecular markers of paraxial mesoderm. Hum Mol Genet. 19:1651–1668. 2010. View Article : Google Scholar : PubMed/NCBI
18	Li N, Yang R, Zhang W, Dorfman H, Rao P and Gorlick R: Genetically transforming human mesenchymal stem cells to sarcomas: changes in cellular phenotype and multilineage differentiation potential. Cancer. 115:4795–4806. 2009. View Article : Google Scholar
19	Varma S, Gupta S, Talwar J, Forte F and Dhar M: Renal epithelioid angiomyolipoma: a malignant disease. J Nephrol. 24:18–22. 2011. View Article : Google Scholar : PubMed/NCBI
20	Paradis V, Laurendeau I, Vieillefond A, Blanchet P, Eschwege P, Benoît G, et al: Clonal analysis of renal sporadic angiomyolipomas. Hum Pathol. 29:1063–1067. 1998. View Article : Google Scholar : PubMed/NCBI
21	Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, et al: A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 3:301–313. 2008. View Article : Google Scholar : PubMed/NCBI
22	Crisan M, Chen CW, Corselli M, Andriolo G, Lazzari L and Péault B: Perivascular multipotent progenitor cells in human organs. Ann NY Acad Sci. 1176:118–123. 2009. View Article : Google Scholar : PubMed/NCBI
23	Corselli M, Chen CW, Crisan M, Lazzari L and Péault B: Perivascular ancestors of adult multipotent stem cells. Arterioscler Thromb Vasc Biol. 30:1104–1109. 2010. View Article : Google Scholar : PubMed/NCBI
24	Mues AC, Palacios JM, Haramis G, Casazza C, Badani K, Gupta M, et al: Contemporary experience in the management of angiomyolipoma. J Endourol. 24:1883–1886. 2010. View Article : Google Scholar : PubMed/NCBI