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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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September 2012 Volume 4 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

The promoter methylation of the Syk gene in nasopharyngeal carcinoma cell lines

  • Authors:
    • Chong Yan
    • Chibo Liu
    • Qiaozhi Jin
    • Zhihai Li
    • Baohong Tao
    • Zhiyi Cai

  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology - Head and Neck Surgery, Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China, Department of Clinical Laboratory, Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China
  • Pages: 505-508
    |
    Published online on: June 19, 2012
       https://doi.org/10.3892/ol.2012.763
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Abstract

The aim of this study was to investigate the mRNA and protein expression levels of the Syk gene as well as its promoter methylation in nasopharyngeal carcinoma (NPC) cell lines. The CNE-1 (highly differentiated), CNE-2 (poorly differentiated) and NP69 (non-cancerous human immortalized nasopharyngeal epithelial cells) cell lines were used in the present study. The MS-PCR, Q-RT-PCR and western blotting methods were used to examine the Syk gene promoter methylation levels and mRNA and protein expression in the three cell lines. The promoter methylation levels in CNE-1, CNE-2 and NP69 cells were 36%, 62% and 0, respectively. The mRNA levels in CNE-1 and CNE-2 cells were 42±3.5 and 28±2% of that in NP69, respectively; the protein levels in CNE-1 and CNE-2 cells were 36±4.5 and 16±2.5 of that in NP69, respectively; the statistical differences between groups were significant. The lower differentiation levels of the NPC cell lines correlate with lower levels of mRNA and protein expression of the Syk gene, as well as higher promoter methylation levels.
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Copy and paste a formatted citation
Spandidos Publications style
Yan C, Liu C, Jin Q, Li Z, Tao B and Cai Z: The promoter methylation of the Syk gene in nasopharyngeal carcinoma cell lines. Oncol Lett 4: 505-508, 2012.
APA
Yan, C., Liu, C., Jin, Q., Li, Z., Tao, B., & Cai, Z. (2012). The promoter methylation of the Syk gene in nasopharyngeal carcinoma cell lines. Oncology Letters, 4, 505-508. https://doi.org/10.3892/ol.2012.763
MLA
Yan, C., Liu, C., Jin, Q., Li, Z., Tao, B., Cai, Z."The promoter methylation of the Syk gene in nasopharyngeal carcinoma cell lines". Oncology Letters 4.3 (2012): 505-508.
Chicago
Yan, C., Liu, C., Jin, Q., Li, Z., Tao, B., Cai, Z."The promoter methylation of the Syk gene in nasopharyngeal carcinoma cell lines". Oncology Letters 4, no. 3 (2012): 505-508. https://doi.org/10.3892/ol.2012.763
Copy and paste a formatted citation
x
Spandidos Publications style
Yan C, Liu C, Jin Q, Li Z, Tao B and Cai Z: The promoter methylation of the Syk gene in nasopharyngeal carcinoma cell lines. Oncol Lett 4: 505-508, 2012.
APA
Yan, C., Liu, C., Jin, Q., Li, Z., Tao, B., & Cai, Z. (2012). The promoter methylation of the Syk gene in nasopharyngeal carcinoma cell lines. Oncology Letters, 4, 505-508. https://doi.org/10.3892/ol.2012.763
MLA
Yan, C., Liu, C., Jin, Q., Li, Z., Tao, B., Cai, Z."The promoter methylation of the Syk gene in nasopharyngeal carcinoma cell lines". Oncology Letters 4.3 (2012): 505-508.
Chicago
Yan, C., Liu, C., Jin, Q., Li, Z., Tao, B., Cai, Z."The promoter methylation of the Syk gene in nasopharyngeal carcinoma cell lines". Oncology Letters 4, no. 3 (2012): 505-508. https://doi.org/10.3892/ol.2012.763
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