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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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September 2012 Volume 4 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Article

miR-96 regulates FOXO1‑mediated cell apoptosis in bladder cancer

  • Authors:
    • Yan Guo
    • Huihui Liu
    • Hui Zhang
    • Chao Shang
    • Yongsheng Song
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, School of Stomatology, China Medical University, Shenyang, Liaoning 110002, P.R. China, Department of Basic Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110847, P.R. China , Department of Urinary Surgery, The Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China , Department of Neurobiology, China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 561-565
    |
    Published online on: June 27, 2012
       https://doi.org/10.3892/ol.2012.775
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Abstract

Transitional cell carcinoma (TCC) is one of the most common types of malignancies and a leading cause of genitourinary system cancer mortality worldwide. The tumor suppressor gene FOXO1, a member of the forkhead box O (FOXO) subfamily of transcription factors, is downregulated in a number of cancers, including TCC; however, the underlying mechanisms are poorly understood. In the present study, we used microRNA (miRNA) target prediction algorithms to identify a conserved potential miR‑96 binding site in the 3'-untranslated region (3'‑UTR) of FOXO1. Using quantitative real‑time PCR (qRT‑PCR) and northern blot analysis, we identified that miR‑96 was downregulated in TCC tissues compared to normal bladder tissues (NB), suggesting that the loss of FOXO1 expression in TCC may be mediated by miR‑96. To confirm this, we transfected pre‑miR‑96/anti‑miR‑96 into the T24 TCC cell line and revealed that miR‑96 expression was sufficient to significantly reduce FOXO1 expression. Conversely, FOXO1 expression was not completely restored by the inhibition of miR‑96 in T24 cells. Moreover, RNA silencing of FOXO1 significantly reduced miR‑96 inhibitor‑mediated T24 cell apoptosis. In conclusion, our study demonstrates that the miR‑96 targeting of FOXO1 is upregulated in TCC; in addition, TCC tumorigenesis may be partly due to the ability of miR-96 to promote FOXO1 repression, thereby bypassing cell apoptosis controls.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Y, Liu H, Zhang H, Shang C and Song Y: miR-96 regulates FOXO1‑mediated cell apoptosis in bladder cancer. Oncol Lett 4: 561-565, 2012.
APA
Guo, Y., Liu, H., Zhang, H., Shang, C., & Song, Y. (2012). miR-96 regulates FOXO1‑mediated cell apoptosis in bladder cancer. Oncology Letters, 4, 561-565. https://doi.org/10.3892/ol.2012.775
MLA
Guo, Y., Liu, H., Zhang, H., Shang, C., Song, Y."miR-96 regulates FOXO1‑mediated cell apoptosis in bladder cancer". Oncology Letters 4.3 (2012): 561-565.
Chicago
Guo, Y., Liu, H., Zhang, H., Shang, C., Song, Y."miR-96 regulates FOXO1‑mediated cell apoptosis in bladder cancer". Oncology Letters 4, no. 3 (2012): 561-565. https://doi.org/10.3892/ol.2012.775
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Y, Liu H, Zhang H, Shang C and Song Y: miR-96 regulates FOXO1‑mediated cell apoptosis in bladder cancer. Oncol Lett 4: 561-565, 2012.
APA
Guo, Y., Liu, H., Zhang, H., Shang, C., & Song, Y. (2012). miR-96 regulates FOXO1‑mediated cell apoptosis in bladder cancer. Oncology Letters, 4, 561-565. https://doi.org/10.3892/ol.2012.775
MLA
Guo, Y., Liu, H., Zhang, H., Shang, C., Song, Y."miR-96 regulates FOXO1‑mediated cell apoptosis in bladder cancer". Oncology Letters 4.3 (2012): 561-565.
Chicago
Guo, Y., Liu, H., Zhang, H., Shang, C., Song, Y."miR-96 regulates FOXO1‑mediated cell apoptosis in bladder cancer". Oncology Letters 4, no. 3 (2012): 561-565. https://doi.org/10.3892/ol.2012.775
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