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Article

Evaluation of microsatellite instability in women with epithelial ovarian cancer

  • Authors:
    • Leonardo Pandolfi Caliman
    • Rubens Lene Carvalho Tavares
    • Josiane   Barbosa Piedade
    • Ana Carolina Silvano Couto de Assis
    • Karen de Jesus Dias da Cunha
    • Letícia  da Conceição Braga
    • Luciana Maria Silva
    • Agnaldo Lopes da Silva Filho
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Faculty of Medicine, Federal University of Minas Gerais (UFMG), 30130-100, Brazil, Research and Development Center, Ezequiel Dias Foundation, 30510-010, Belo Horizonte, Minas Gerais, Brazil
  • Pages: 556-560
    |
    Published online on: June 27, 2012
       https://doi.org/10.3892/ol.2012.776
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Abstract

The function of microsatellite instability (MSI) and the optimal panel of markers for epithelial ovarian cancer (EOC) are not well established. This study aimed to use the National Cancer Institute (NCI) markers BAT25, BAT26, D2S123, D5S346 and D17S250 to evaluate MSI in patients with ovarian serous cystadenocarcinoma, compared with ovarian serous cystadenoma and normal ovaries. A total of 37 patients were divided into three groups, as follows: cystadenocarcinoma (n=13), cystadenoma (n=10) and normal ovaries (n=14). DNA was extracted with TRIzol and quantified by spectrophotometry. MSI was evaluated by polymerase chain reaction (PCR), and classified as high (MSI-H), low (MSI-L) or stable (MSS). FIGO staging was I/II in 23.1% and III/IV in 76.9% of the cystadenocarcinoma group. Polymorphisms were found using at least one marker in 32 women, and were observed with D2S123 (83.7%), D17S250 (81.1%), D5S346 (72.9%), BAT25 (21.6%) and BAT26 (16.2%) markers. In the cystadenocarcinoma group, BAT25, BAT26, D2S123, D5S346 and D17S250 markers were positive in 30.8, 76.9, 53.8, 69.2 and 69.2% of patients, respectively. The same markers were positive in 30, 50, 40, 60 and 30% of the cystadenoma group, and 50, 71.4, 71.4, 64.3 and 63.3% in the normal ovary group, respectively. MSI-H was present in 84.6, 60 and 78.6% of the cystadenocarcinoma, cystadenoma and normal patients, respectively. MSI-L was detected in 0, 30 and 7.1%, and MSS was identified in 15.4, 10 and 14.3% of the cystadenocarcinoma, cystadenoma and normal patients, respectively. The frequency of MSI in both benign epithelial ovarian neoplasms and in normal ovaries was high, as well as in EOC, with no statistically significant difference between the groups. This suggests that MSI may arise as a consequence of the ovulatory process, and not solely as a feature of malignant ovarian tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Caliman LP, Carvalho Tavares RL, Barbosa Piedade J, Silvano Couto de Assis AC, de Jesus Dias da Cunha K, da Conceição Braga L, Silva LM and da Silva Filho AL: Evaluation of microsatellite instability in women with epithelial ovarian cancer. Oncol Lett 4: 556-560, 2012.
APA
Caliman, L.P., Carvalho Tavares, R.L., Barbosa Piedade, J., Silvano Couto de Assis, A.C., de Jesus Dias da Cunha, K., da Conceição Braga, L. ... da Silva Filho, A.L. (2012). Evaluation of microsatellite instability in women with epithelial ovarian cancer. Oncology Letters, 4, 556-560. https://doi.org/10.3892/ol.2012.776
MLA
Caliman, L. P., Carvalho Tavares, R. L., Barbosa Piedade, J., Silvano Couto de Assis, A. C., de Jesus Dias da Cunha, K., da Conceição Braga, L., Silva, L. M., da Silva Filho, A. L."Evaluation of microsatellite instability in women with epithelial ovarian cancer". Oncology Letters 4.3 (2012): 556-560.
Chicago
Caliman, L. P., Carvalho Tavares, R. L., Barbosa Piedade, J., Silvano Couto de Assis, A. C., de Jesus Dias da Cunha, K., da Conceição Braga, L., Silva, L. M., da Silva Filho, A. L."Evaluation of microsatellite instability in women with epithelial ovarian cancer". Oncology Letters 4, no. 3 (2012): 556-560. https://doi.org/10.3892/ol.2012.776
Copy and paste a formatted citation
x
Spandidos Publications style
Caliman LP, Carvalho Tavares RL, Barbosa Piedade J, Silvano Couto de Assis AC, de Jesus Dias da Cunha K, da Conceição Braga L, Silva LM and da Silva Filho AL: Evaluation of microsatellite instability in women with epithelial ovarian cancer. Oncol Lett 4: 556-560, 2012.
APA
Caliman, L.P., Carvalho Tavares, R.L., Barbosa Piedade, J., Silvano Couto de Assis, A.C., de Jesus Dias da Cunha, K., da Conceição Braga, L. ... da Silva Filho, A.L. (2012). Evaluation of microsatellite instability in women with epithelial ovarian cancer. Oncology Letters, 4, 556-560. https://doi.org/10.3892/ol.2012.776
MLA
Caliman, L. P., Carvalho Tavares, R. L., Barbosa Piedade, J., Silvano Couto de Assis, A. C., de Jesus Dias da Cunha, K., da Conceição Braga, L., Silva, L. M., da Silva Filho, A. L."Evaluation of microsatellite instability in women with epithelial ovarian cancer". Oncology Letters 4.3 (2012): 556-560.
Chicago
Caliman, L. P., Carvalho Tavares, R. L., Barbosa Piedade, J., Silvano Couto de Assis, A. C., de Jesus Dias da Cunha, K., da Conceição Braga, L., Silva, L. M., da Silva Filho, A. L."Evaluation of microsatellite instability in women with epithelial ovarian cancer". Oncology Letters 4, no. 3 (2012): 556-560. https://doi.org/10.3892/ol.2012.776
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