Deguelin, a natural rotenoid, inhibits mouse myeloma cell growth in vitro via induction of apoptosis

Li,Zhengguang; Wu,Jun; Wu,Changping; Jiang,Jingting; Zheng,Xiao; Xu,Bin; Li,Min

doi:10.3892/ol.2012.790

Deguelin, a natural rotenoid, inhibits mouse myeloma cell growth in vitro via induction of apoptosis

Authors:
- Zhengguang Li
- Jun Wu
- Changping Wu
- Jingting Jiang
- Xiao Zheng
- Bin Xu
- Min Li
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Affiliations: Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou 213003, P.R. China
Published online on: July 6, 2012 https://doi.org/10.3892/ol.2012.790
Pages: 677-681

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Abstract

Deguelin is a naturally occurring rotenoid with strong cancer chemopreventive and antitumor activities. In the present study, we investigated the antitumor activity of deguelin against MPC-11 murine myeloma cells and the possible mechanism of action in vitro. Our results revealed that deguelin inhibited the proliferation of MPC-11 cells in a concentration- and time-dependent manner and caused the apoptotic death of MPC-11 cells. Following exposure to deguelin, the phosphorylation of Akt was decreased. The inhibition of cell growth may be associated with decreased levels of phosphorylated Akt. Deguelin-induced apoptosis was characterized by the upregulation of Bax, downregulation of Bcl-2 and activation of caspase-3. In conclusion, deguelin inhibits murine myeloma cell proliferation by inducing apoptosis via regulation of the Bax/Bcl-2 ratio and by inhibition of the activation of Akt. Its potential as an anticancer agent against multiple myeloma warrants further investigation.

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