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Article

Depletion of Bmi-1 enhances 5-fluorouracil-induced apoptosis and autophagy in hepatocellular carcinoma cells

  • Authors:
    • Jing Wu
    • Dong Hu
    • Rongbo Zhang

  • View Affiliations / Copyright

    Affiliations: Department of Medical Immunology, School of Medicine, Anhui University of Science and Technology, Huainan, Anhui 232001, P.R. China
  • Pages: 723-726
    |
    Published online on: July 16, 2012
       https://doi.org/10.3892/ol.2012.805
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Abstract

5-fluorouracil (5‑FU) is one of the standard chemoradiotherapy regimens for hepatocellular carcinoma (HCC) treatment. B‑cell‑specific Moloney murine leukemia virus insertion site 1 (Bmi‑1) has been demonstrated to regulate proliferation. Additionally, Bmi‑1 overexpression has been identified in HCC cell lines and correlates with the advanced invasive stage of tumor progression and poor prognosis. In this study, we examined the effects of 5‑FU treatment on cell growth in HCC cells with or without Bmi‑1 depletion. The IC50 values of 5‑FU were significantly decreased to a greater extent in cells with Bmi‑1 knockdown. Depletion of Bmi‑1 increased sensitivity of the cells to 5‑FU and increased apoptosis. Knockdown of endogenous Bmi‑1 led to a substantial reduction in the levels of phospho‑AKT and Bcl‑2 with a concomitant increase in the levels of Bax. Additionally, 5‑FU induced the conversion/turnover of microtubule-associated protein 1 light chain 3 (LC3). Knockdown of endogenous Bmi‑1 led to an increase in the levels of Beclin‑1 and the accumulation of LC3‑II. Together, these findings reveal that Bmi‑1 depletion enhanced the chemosensitivity of HCC cells by inducing apoptosis and autophagy, which is associated with the PI3K/AKT and Bcl‑2/Beclin‑1 pathways.
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1. 

H NaganoTreatment of advanced hepatocellular carcinoma: intraarterial infusion chemotherapy combined with interferonOncology78Suppl 1142147201010.1159/000315243

2. 

JM LIovetJ BruixMolecular targeted therapies in hepatocellular carcinomaHepatology4813121327200810.1002/hep.2250618821591

3. 

M BoulinS GuiuB ChauffertS AhoJP CercueilF GhiringhelliD KrauseP FagnoniP HillonL BedenneB GuiuScreening of anticancer drugs for chemoembolization of hepatocellular carcinomaAnticancer Drugs22741748201110.1097/CAD.0b013e328346a0c521487286

4. 

Y HauptWS AlexanderG BarriSP KlinkenJM AdamsNovel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in E mu-myc transgenic miceCell65753763199110.1016/0092-8674(91)90383-A1904009

5. 

M van LohuizenS VerbeekB ScheijenE WientjensH van der GuldenA BernsIdentification of cooperating oncogenes in E mu-myc transgenic mice by provirus taggingCell6573775219911904008

6. 

ME Valk-LingbeekSW BruggemanM van LohuizenStem cells and cancer; the polycomb connectionCell118409418200410.1016/j.cell.2004.08.00515315754

7. 

MJ GunsterFM RaaphorstKM HamerJL den BlaauwenE FieretCJ MeiierAP OtteDifferential expression of human polycomb group proteins in various tissues and cell typesJ Cell Biochem Suppl36129143200110.1002/jcb.109311455578

8. 

H WangK PanHK ZhangDS WengJ ZhouJJ LiW HuangHF SongMS ChenJC XiaIncreased polycomb-group oncogene Bmi-1 expression correlates with poor prognosis in hepatocellular carcinomaJ Cancer Res Clin Oncol134535541200810.1007/s00432-007-0316-817917742

9. 

L LiuLG AndrewsTO TollefsbolLoss of the human polycomb group protein BMI1 promotes cancer-specific cell deathOncogene2543704375200610.1038/sj.onc.120945416501599

10. 

J WuD HuG YangJ ZhouC YangY GaoZ ZhuDown-regulation of Bmi-1 cooperates with artemisinin on growth inhibition of nasopharyngeal carcinoma cellsJ Cell Biochem11219381948201110.1002/jcb.2311421445878

11. 

JE ChipukDR GreenHow do Bcl-2 proteins induce mitochondrial outer membrane permeabilization?Trends Cell Biol18157164200810.1016/j.tcb.2008.01.00718314333

12. 

S WhittakerR MaraisAX ZhuThe role of signaling pathways in the development and treatment of hepatocellular carcinomaOncogene2949895005201010.1038/onc.2010.23620639898

13. 

YL ChenPY LawHH LohInhibition of PI3K/Akt signaling: an emerging paradigm for targeted cancer therapyCurr Med Chem Anticancer Agents5575589200510.2174/15680110577457464916305480

14. 

JM GumpA ThorburnAutophagy and apoptosis: what is the connection?Trends Cell Biol21387392201110.1016/j.tcb.2011.03.00721561772

15. 

F ZhouY YangD XingBcl-2 and Bcl-xl play important roles in the crosstalk between autophagy and apoptosisFEBS J278403413201110.1111/j.1742-4658.2010.07965.x21182587

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Copy and paste a formatted citation
Spandidos Publications style
Wu J, Hu D and Zhang R: Depletion of Bmi-1 enhances 5-fluorouracil-induced apoptosis and autophagy in hepatocellular carcinoma cells. Oncol Lett 4: 723-726, 2012.
APA
Wu, J., Hu, D., & Zhang, R. (2012). Depletion of Bmi-1 enhances 5-fluorouracil-induced apoptosis and autophagy in hepatocellular carcinoma cells. Oncology Letters, 4, 723-726. https://doi.org/10.3892/ol.2012.805
MLA
Wu, J., Hu, D., Zhang, R."Depletion of Bmi-1 enhances 5-fluorouracil-induced apoptosis and autophagy in hepatocellular carcinoma cells". Oncology Letters 4.4 (2012): 723-726.
Chicago
Wu, J., Hu, D., Zhang, R."Depletion of Bmi-1 enhances 5-fluorouracil-induced apoptosis and autophagy in hepatocellular carcinoma cells". Oncology Letters 4, no. 4 (2012): 723-726. https://doi.org/10.3892/ol.2012.805
Copy and paste a formatted citation
x
Spandidos Publications style
Wu J, Hu D and Zhang R: Depletion of Bmi-1 enhances 5-fluorouracil-induced apoptosis and autophagy in hepatocellular carcinoma cells. Oncol Lett 4: 723-726, 2012.
APA
Wu, J., Hu, D., & Zhang, R. (2012). Depletion of Bmi-1 enhances 5-fluorouracil-induced apoptosis and autophagy in hepatocellular carcinoma cells. Oncology Letters, 4, 723-726. https://doi.org/10.3892/ol.2012.805
MLA
Wu, J., Hu, D., Zhang, R."Depletion of Bmi-1 enhances 5-fluorouracil-induced apoptosis and autophagy in hepatocellular carcinoma cells". Oncology Letters 4.4 (2012): 723-726.
Chicago
Wu, J., Hu, D., Zhang, R."Depletion of Bmi-1 enhances 5-fluorouracil-induced apoptosis and autophagy in hepatocellular carcinoma cells". Oncology Letters 4, no. 4 (2012): 723-726. https://doi.org/10.3892/ol.2012.805
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