Study of the correlation between H-ras mutation and primary hepatocellular carcinoma

  • Authors:
    • Guode Sui
    • Xuexiao Ma
    • Shiguo Liu
    • Haitao Niu
    • Qian Dong
  • View Affiliations

  • Published online on: July 27, 2012     https://doi.org/10.3892/ol.2012.832
  • Pages: 779-782
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Abstract

The aim of this study was to investigate the correlation between H-ras mutation and primary hepatocellular carcinoma (HCC) and to describe the role of H-ras mutation in carcinogenesis. Clinical samples of 69 patients were collected and the expression levels of H-ras in HCC and the surrounding normal tissues were examined using HotStarTaq PCR. H-ras mutation was further analyzed using the PCR direct sequencing method. The results showed that H-ras mutation was present in 49 samples (49/69, 71.01%), of which 19 had codon 40 mutated from CTA to CTG and 30 had codon 61 mutated from GGC to AGC. By contrast, only 2 mutations were found in the normal tumor-adjacent tissues. The H-ras mutation rate in the high-risk of metastatic recurrence group was markedly higher than that in the low-risk group (P<0.01). The H-ras mutation rate in patients with metastatic recurrence during postoperative follow-up was also significantly higher than that in patients without metastatic recurrence (P<0.01). Based on the above results, the H-ras mutation frequency in cancer tissues is markedly higher compared with that in normal tissues. H-ras mutation may play an important role in the genesis and development of HCC and may serve as a reliable marker for individual comprehensive therapy in HCC.
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October 2012
Volume 4 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Sui G, Ma X, Liu S, Niu H and Dong Q: Study of the correlation between H-ras mutation and primary hepatocellular carcinoma. Oncol Lett 4: 779-782, 2012
APA
Sui, G., Ma, X., Liu, S., Niu, H., & Dong, Q. (2012). Study of the correlation between H-ras mutation and primary hepatocellular carcinoma. Oncology Letters, 4, 779-782. https://doi.org/10.3892/ol.2012.832
MLA
Sui, G., Ma, X., Liu, S., Niu, H., Dong, Q."Study of the correlation between H-ras mutation and primary hepatocellular carcinoma". Oncology Letters 4.4 (2012): 779-782.
Chicago
Sui, G., Ma, X., Liu, S., Niu, H., Dong, Q."Study of the correlation between H-ras mutation and primary hepatocellular carcinoma". Oncology Letters 4, no. 4 (2012): 779-782. https://doi.org/10.3892/ol.2012.832