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Article

Effect of CCR7, CXCR4 and VEGF‑C on the lymph node metastasis of human pancreatic ductal adenocarcinoma

  • Authors:
    • Jinghui Guo
    • Wenhui Lou
    • Yuan Ji
    • Shuncai Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, P.R. China, Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University, Shanghai, P.R. China, Department of Pathology, Zhongshan Hospital Affiliated to Fudan University, Shanghai, P.R. China, Department of Gastroenterology, Zhongshan Hospital Affiliated to Fudan University, Shanghai, P.R. China
  • Pages: 1572-1578
    |
    Published online on: March 15, 2013
       https://doi.org/10.3892/ol.2013.1261
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Abstract

The aim of the present study was to investigate the association between the expression of chemokine receptors CCR7 and CXCR4 and vascular endothelial growth factor (VEGF)‑C and the lymph node metastasis of pancreatic ductal adenocarcinoma (PDAC). The mRNA transcription levels of CCR7, CXCR4 and VEGF‑C were measured in 24 specimens by real‑time reverse transcription (RT)‑PCR, while the protein expression levels were measured in 65 specimens by immuohistochemistry. Professional software for pathological image manipulation (Image Pro Plus 6.0) was used to quantitate the results of the immunohistochemical staining. The mRNA and protein expression levels of CCR7, CXCR4 and VEGF‑C were all significantly higher in the cancer samples compared with those in the adjacent normal tissue. The CCR7 and VEGF‑C mRNA and protein expression levels were significantly higher in the patients with cancer types exhibiting lymph node metastasis and an advanced International Union Against Cancer (UICC) stage (P<0.05). The greater the number of metastatic lymph nodes, the higher the levels of CCR7 expression (P<0.05). There was a significant positive linear correlation between the mRNA and protein expression levels of CCR7 and VEGF‑C (P<0.05). The mRNA and protein expression levels of CXCR4 were not correlated with the lymph node metastasis (P>0.05), however the strong positive expression of CCR7 and VEGF‑C was significantly associated with the lymph node metastasis of PDAC.
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Copy and paste a formatted citation
Spandidos Publications style
Guo J, Lou W, Ji Y and Zhang S: Effect of CCR7, CXCR4 and VEGF‑C on the lymph node metastasis of human pancreatic ductal adenocarcinoma. Oncol Lett 5: 1572-1578, 2013.
APA
Guo, J., Lou, W., Ji, Y., & Zhang, S. (2013). Effect of CCR7, CXCR4 and VEGF‑C on the lymph node metastasis of human pancreatic ductal adenocarcinoma. Oncology Letters, 5, 1572-1578. https://doi.org/10.3892/ol.2013.1261
MLA
Guo, J., Lou, W., Ji, Y., Zhang, S."Effect of CCR7, CXCR4 and VEGF‑C on the lymph node metastasis of human pancreatic ductal adenocarcinoma". Oncology Letters 5.5 (2013): 1572-1578.
Chicago
Guo, J., Lou, W., Ji, Y., Zhang, S."Effect of CCR7, CXCR4 and VEGF‑C on the lymph node metastasis of human pancreatic ductal adenocarcinoma". Oncology Letters 5, no. 5 (2013): 1572-1578. https://doi.org/10.3892/ol.2013.1261
Copy and paste a formatted citation
x
Spandidos Publications style
Guo J, Lou W, Ji Y and Zhang S: Effect of CCR7, CXCR4 and VEGF‑C on the lymph node metastasis of human pancreatic ductal adenocarcinoma. Oncol Lett 5: 1572-1578, 2013.
APA
Guo, J., Lou, W., Ji, Y., & Zhang, S. (2013). Effect of CCR7, CXCR4 and VEGF‑C on the lymph node metastasis of human pancreatic ductal adenocarcinoma. Oncology Letters, 5, 1572-1578. https://doi.org/10.3892/ol.2013.1261
MLA
Guo, J., Lou, W., Ji, Y., Zhang, S."Effect of CCR7, CXCR4 and VEGF‑C on the lymph node metastasis of human pancreatic ductal adenocarcinoma". Oncology Letters 5.5 (2013): 1572-1578.
Chicago
Guo, J., Lou, W., Ji, Y., Zhang, S."Effect of CCR7, CXCR4 and VEGF‑C on the lymph node metastasis of human pancreatic ductal adenocarcinoma". Oncology Letters 5, no. 5 (2013): 1572-1578. https://doi.org/10.3892/ol.2013.1261
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