Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
June 2013 Volume 5 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June 2013 Volume 5 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Cytotoxicity of cytokine‑induced killer cells targeted by a bispecific antibody to gastric cancer cells

  • Authors:
    • Lin Zhang
    • Yanhong Hou
    • Jian Zhang
    • Jing Hu
    • Kunpeng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The 309 Hospital of the PLA, Beijing 100091, P.R. China, Department of Molecular Biology, The Fourth Military Medical University, Xi'an 710032, P.R. China
  • Pages: 1826-1832
    |
    Published online on: April 2, 2013
       https://doi.org/10.3892/ol.2013.1281
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the cytotoxic activity of cytokine‑induced killer (CIK) cells targeted by an epidermal growth factor receptor (EGFR)/CD3 bispecific antibody (BsAb) to the gastric cancer cell line SGC7901. A BsAb was constructed by chemically crosslinking a monoclonal antibody (McAb) against human CD3 with another McAb against human EGFR. An immunocytochemistry assay was performed to detect the expression of EGFR in SGC7901 cells. The cytotoxic activity of CIK cells targeted by the EGFR/CD3 BsAb was analyzed by the 51Cr release assay, Subsequently, a comparison of the cytotoxic activity between CIK cells targeted by EGFR/CD3 BsAb, CIK cells targeted by EGFR McAb or/and CD3 McAb and CIK cells was performed. The antineoplastic activity of the CIK cells directed using the EGFR/CD3 BsAb in vivo was analyzed by tumor growth and tumor reduction assays. The cell lysis rate of CIK cells targeted by the EGFR/CD3 BsAb was higher compared with those of CIK cells targeted by CD3 McAb only or by CD3 McAb and EGFR McAb. The lysis rates of the latter two groups were significantly higher than those of CIK cells targeted by EGFR McAb only and CIK cells (P<0.05). The mean tumor reduction using the administration of CIK cells directed by the EGFR/CD3 BsAb was higher than those of the other groups (P<0.05). The results indicate that the EGFR/CD3 BsAb is able to enhance the ability of CIK cells to bind to and kill gastric cancer cells in vitro and in vivo.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

View References

1 

Parkin DM, Poisani P and Ferlay: Global cancer statistics. CA Cancer J Clin. 49:33–64. 1999. View Article : Google Scholar

2 

Stein HJ, Sendler A, Fink U and Siewert JR: Multidisciplinary approach to esophageal and gastric cancer. Surg Clin North Am. 80:659–682. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Lu PH and Negrin RS: A novel population of expanded human CD3+CD56+ cells derived from T cells with potent in vivo antitumor activity in mice with severe combined immunodeficiency. J Immunol. 153:1687–1696. 1994.PubMed/NCBI

4 

Schmidt-Wolf IG, Lefterova P, Mehta BA, et al: Phenotypic characterization and identification of effector cells involved in tumor cell recognition of cytokine-induced killer cells. Exp Hematol. 21:1673–1679. 1993.PubMed/NCBI

5 

Märten A, Ziske C, Schöttker B, et al: Interactions between dendritic cells and cytokine-induced killer cells lead to an activation of both populations. J Immunother. 24:502–510. 2001.PubMed/NCBI

6 

Hui D, Qiang L, Jian W, et al: A randomized, controlled trial of postoperative adjuvant cytokine-induced killer cells immunotherapy after radical resection of hepatocellular carcinoma. Dig Liver Dis. 41:36–41. 2009. View Article : Google Scholar

7 

Li H, Wang C, Yu J, et al: Dendritic cell-activated cytokine-induced killer cells enhance the anti-tumor effect of chemotherapy on non-small cell lung cancer in patients after surgery. Cytotherapy. 11:1076–1083. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Beun GD, van de Welde CJ and Fleuren GJ: T-cell based cancer immunotherapy: direct or redirected tumor-cell recognition? Immunol Today. 15:11–15. 1994. View Article : Google Scholar : PubMed/NCBI

9 

Fury MG, Lipton A, Smith KM, Winston CB and Pfister DG: A phase-I trial of the epidermal growth factor receptor directed bispecic antibody MDX-447 without and with recombinant human granulocytecolony stimulating factor in patients with advanced solid tumors. Cancer Immunol Immunother. 57:155–163. 2008. View Article : Google Scholar

10 

Gall JM, Davol PA, Grabert RC, Deaver M and Lum LG: T cells armed with anti-CD3 × anti-CD20 bispecific antibody enhance killing of CD20+ malignant B cells and bypass complement-mediated rituximab resistance in vitro. Exp Hematol. 33:452–459. 2005.

11 

Stanglmaier M, Faltin M, Ruf P, Bodenhausen A, Schröder P and Lindhofer H: Bi20 (fBTA05), a novel trifunctional bispecific antibody (anti-CD20 3 anti-CD3), mediates efficient killing of B-cell lymphoma cells even with very low CD20 expression levels. Int J Cancer. 123:1181–1189. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Wimberger P, Xiang W, Mayr D, Diebold J, Dreier T, Baeuerle PA and Kimmig R: Efficient tumor cell lysis by autologous, tumor-resident T-lymphocytes in primary ovarian cancer samples by an EP-CAM-/CD3-bispecific antibody. Int J Cancer. 105:241–248. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Morecki S, Lindhofer H, Yacovlev E, Gelfand Y, Ruf P and Slavin S: Induction of long-lasting antitumor immunity by concomitant cell therapy with allogeneic lymphocytes and trifunctional bispecific antibody. Exp Hematol. 36:997–1003. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Sen M, Wankowski DM, Garlie NK, et al: Use of anti-CD3 × anti-HER2/neu bispecific antibody for redirecting cytotoxicity of activated T cells toward HER2/neu+ tumors. J Hematother Stem Cell Res. 10:247–260. 2001.

15 

Tita-Nwa F, Moldenhauer G, Herbst M, Kleist C, Ho AD and Kornacker M: Cytokine-induced killer cells targeted by the novel bispecific antibody CD19xCD5 (HD37xT5.16) efficiently lyse B-lymphoma cells. Cancer Immunol Immunother. 56:1911–1920. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Hoyle C, Bangs CD, Chang P, et al: Expansion of Philadelphia chromosome-negative CD3(+) CD56(+) cytotoxic cells from chronic myeloid leukemia patients: in vitro and in vivo efficacy in severe combined immunodeficiency disease mice. Blood. 92:3318–33127. 1998.PubMed/NCBI

17 

Chan JK, Hamilton CA, Cheung MK, Karimi M, Baker J, Gall JM, Schulz S, Thorne SH, Teng NN, Contag CH, Lum LG and Negrin RS: Enhanced killing of primary ovarian cancer by retargeting autologous cytokine-induced killer cells with bispecific antibodies: a preclinical study. Clin Cancer Res. 12:1859–1867. 2006. View Article : Google Scholar

18 

Mehta BA, Schmidt-Wolf IG, Weissman IL and Negrin RS: Two pathways of exocytosis of cytoplasmic granule contents and target cell killing by cytokine-induced CD3+ CD56+ killer cells. Blood. 86:3493–3499. 1995.PubMed/NCBI

19 

Schmidt-Wolf GD, Negrin RS and Schmidt-Wolf IG: Activated. T cells and cytokine-induced CD3+CD56+ killer cells. Ann Hematol. 74:51–56. 1997. View Article : Google Scholar : PubMed/NCBI

20 

Schmidt-Wolf IG, Lefterova P, Johnston V, Huhn D, Blume KG and Negrin RS: Propagation of large numbers of T cells with natural killer cell markers. Br J Haematol. 87:453–458. 1994. View Article : Google Scholar : PubMed/NCBI

21 

Canevari S, Stoter G, Arienti F, Bolis G, Colnaghi MI, Di Re EM, Eggermont AM, Goey SH, Gratama JW, Lamers CH, et al: Regression of advanced ovarian carcinoma by intraperitoneal treatment with autologous T lymphocytes retargeted by a bispecific monoclonal antibody. J Natl Cancer Inst. 87:1463–1469. 1995. View Article : Google Scholar

22 

de Gast GC, Haagen IA, van Houten AA, Klein SC, Duits AJ, de Weger RA, Vroom TM, Clark MR, Phillips J and van Dijk AJ: CD8 T cell activation after intravenous administration of CD3 × CD19 bispecific antibody in patients with non-Hodgkin lymphoma. Cancer Immunol Immunother. 40:390–396. 1995.

23 

Kroesen BJ, Bakker A, van Lier RA, et al: Bispecific antibody-mediated target cell-specific costimulation of resting T cells via CD5 and CD28. Cancer Res. 55:4409–4415. 1995.PubMed/NCBI

24 

Marmé A, Strauss G, Bastert G, Grischke EM and Moldenhauer G: Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma. Int J Cancer. 101:183–189. 2002.PubMed/NCBI

25 

Jonker DJ, O’Callaghan CJ, Karapetis CS, et al: Cetuximab for the treatment of colorectal cancer. N Engl J Med. 357:2040–2048. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Fakih MG, Wilding G and Lombardo J: Cetuximab-induced hypomagnesemia in patients with colorectal cancer. Clin Colorectal Cancer. 6:152–156. 2006. View Article : Google Scholar

27 

Katzenwadel A, Schleer H, Gierschner D, Wetterauer U and Elsässer-Beile U: Construction and in vivo evaluation of an anti-PSA x anti-CD3 bispecific antibody for the immunotherapy of prostate cancer. Anticancer Res. 20:1551–1555. 2000.PubMed/NCBI

28 

de Leij L, Molema G, Helfrich W and Kroesen BJ: Bispecific antibodies for treatment of cancer in experimental animal models and man. Adv Drug Deliv Rev. 31:105–129. 1998.PubMed/NCBI

29 

Verneris MR, Arshi A, Edinger M, Kornacker M, Natkunam Y, Karami M, Cao YA, Marina N, Contag CH and Negrin RS: Low levels of Her2/neu expressed by Ewing's family tumor cell lines can redirect cytokine-induced killer cells. Clin Cancer Res. 11:4561–4570. 2005.PubMed/NCBI

30 

Scambia G, Benedetti Panici P, Ferrandina G, et al: Significance of epidermal growth factor receptor expression in primary human endometrial cancer. Int J Cancer. 56:26–30. 1994. View Article : Google Scholar : PubMed/NCBI

31 

Mukohara T, Kudoh S, Yamauchi S, et al: Expression of epidermal growth factor receptor (EGFR) and downstream-activated peptides in surgically excised non-small-cell lung cancer (NSCLC). Lung Cancer. 41:123–130. 2003. View Article : Google Scholar : PubMed/NCBI

32 

Xie FH and Wang RX: The research progress of the relationship between the way of EGFR family’s signal transfer and breast cancer. Int J Lab Med. 27:1004–1008. 2006.

33 

Kameda T, Yasui W, Tsujino T, et al: Tyrosine kinase activity of epidermal growth factor receptor in human gastric carcinomas. Pathol Res Pract. 188:37–43. 1992. View Article : Google Scholar : PubMed/NCBI

34 

Yasui W, Hata T, Yokozaki H, et al: Interaction between epidermal growth factor and its receptor in progression of human gastric carcinoma. Int J Cancer. 41:211–217. 1998. View Article : Google Scholar : PubMed/NCBI

35 

Pinto C, Difabio F, Siena S, et al: Phase II study of cetuximab in combination with FOLFIRI in patients with untreated advanced gastric or gastroesophageal junction adenocarcinoma (FOLCETUX study). Ann Oncol. 18:510–517. 2007. View Article : Google Scholar

36 

Han SW, Oh DY, Im SA, et al: Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer. Br J Cancer. 100:298–304. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Hou Y, Zhang J, Hu J and Zhang K: Cytotoxicity of cytokine‑induced killer cells targeted by a bispecific antibody to gastric cancer cells. Oncol Lett 5: 1826-1832, 2013.
APA
Zhang, L., Hou, Y., Zhang, J., Hu, J., & Zhang, K. (2013). Cytotoxicity of cytokine‑induced killer cells targeted by a bispecific antibody to gastric cancer cells. Oncology Letters, 5, 1826-1832. https://doi.org/10.3892/ol.2013.1281
MLA
Zhang, L., Hou, Y., Zhang, J., Hu, J., Zhang, K."Cytotoxicity of cytokine‑induced killer cells targeted by a bispecific antibody to gastric cancer cells". Oncology Letters 5.6 (2013): 1826-1832.
Chicago
Zhang, L., Hou, Y., Zhang, J., Hu, J., Zhang, K."Cytotoxicity of cytokine‑induced killer cells targeted by a bispecific antibody to gastric cancer cells". Oncology Letters 5, no. 6 (2013): 1826-1832. https://doi.org/10.3892/ol.2013.1281
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Hou Y, Zhang J, Hu J and Zhang K: Cytotoxicity of cytokine‑induced killer cells targeted by a bispecific antibody to gastric cancer cells. Oncol Lett 5: 1826-1832, 2013.
APA
Zhang, L., Hou, Y., Zhang, J., Hu, J., & Zhang, K. (2013). Cytotoxicity of cytokine‑induced killer cells targeted by a bispecific antibody to gastric cancer cells. Oncology Letters, 5, 1826-1832. https://doi.org/10.3892/ol.2013.1281
MLA
Zhang, L., Hou, Y., Zhang, J., Hu, J., Zhang, K."Cytotoxicity of cytokine‑induced killer cells targeted by a bispecific antibody to gastric cancer cells". Oncology Letters 5.6 (2013): 1826-1832.
Chicago
Zhang, L., Hou, Y., Zhang, J., Hu, J., Zhang, K."Cytotoxicity of cytokine‑induced killer cells targeted by a bispecific antibody to gastric cancer cells". Oncology Letters 5, no. 6 (2013): 1826-1832. https://doi.org/10.3892/ol.2013.1281
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team