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Article

Twist2 promotes ovarian cancer cell survival through activation of Akt

  • Authors:
    • Yubin Mao
    • Jinfei Xu
    • Gang Song
    • Nini Zhang
    • Hao Yin
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology in Basic Science, Medical College of Xiamen University, Xiamen, Fujian 361102, P.R. China, Cancer Research Center, Medical College of Xiamen University, Xiamen, Fujian 361102, P.R. China
  • Pages: 169-174
    |
    Published online on: April 24, 2013
       https://doi.org/10.3892/ol.2013.1316
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Abstract

Hypoxia‑inducible factor‑1α (HIF‑1α) is an important prognostic factor in ovarian carcinoma. Hypoxia contributes to tumor progression and is involved in the epithelial‑mesenchymal transition (EMT). Twist2 is an EMT regulator, however, it remains poorly understood in ovarian carcinoma. The present study evaluated the expression of HIF‑1α and Twist2 and further investigated whether Twist2 is involved in hypoxia‑induced apoptosis in ovarian cancer. A series of matched paraffin‑embedded tissue sections from human primary ovarian cancer and normal ovarian tissues were examined through immunohistochemical analysis, a Twist2‑overexpressing stable ovarian cancer cell line was established and deferoxamine (DFO) was introduced to simulate hypoxic conditions. DFO‑induced apoptosis was examined by fluorescence microscopy, MTT assays and flow cytometry. In addition, a western blot analysis was performed to examine the molecular mechanism(s) of action. Twist2 increased in epithelial ovarian cancers associated with HIF‑1α expression. The acquired expression of Twist2 was able to promote the survival of ovarian cancer cells through Akt phosphorylation under DFO‑induced hypoxic stress. The results suggest that Twist2 activates the PI‑3K‑Akt pathway to protect cells from apoptosis in a hypoxic environment. Moreover, Twist2 may be involved in the HIF‑1α signaling pathway in ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Mao Y, Xu J, Song G, Zhang N and Yin H: Twist2 promotes ovarian cancer cell survival through activation of Akt. Oncol Lett 6: 169-174, 2013.
APA
Mao, Y., Xu, J., Song, G., Zhang, N., & Yin, H. (2013). Twist2 promotes ovarian cancer cell survival through activation of Akt. Oncology Letters, 6, 169-174. https://doi.org/10.3892/ol.2013.1316
MLA
Mao, Y., Xu, J., Song, G., Zhang, N., Yin, H."Twist2 promotes ovarian cancer cell survival through activation of Akt". Oncology Letters 6.1 (2013): 169-174.
Chicago
Mao, Y., Xu, J., Song, G., Zhang, N., Yin, H."Twist2 promotes ovarian cancer cell survival through activation of Akt". Oncology Letters 6, no. 1 (2013): 169-174. https://doi.org/10.3892/ol.2013.1316
Copy and paste a formatted citation
x
Spandidos Publications style
Mao Y, Xu J, Song G, Zhang N and Yin H: Twist2 promotes ovarian cancer cell survival through activation of Akt. Oncol Lett 6: 169-174, 2013.
APA
Mao, Y., Xu, J., Song, G., Zhang, N., & Yin, H. (2013). Twist2 promotes ovarian cancer cell survival through activation of Akt. Oncology Letters, 6, 169-174. https://doi.org/10.3892/ol.2013.1316
MLA
Mao, Y., Xu, J., Song, G., Zhang, N., Yin, H."Twist2 promotes ovarian cancer cell survival through activation of Akt". Oncology Letters 6.1 (2013): 169-174.
Chicago
Mao, Y., Xu, J., Song, G., Zhang, N., Yin, H."Twist2 promotes ovarian cancer cell survival through activation of Akt". Oncology Letters 6, no. 1 (2013): 169-174. https://doi.org/10.3892/ol.2013.1316
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