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Article

Purification of NAD+ glycohydrolase from human serum

  • Authors:
    • Özlem Coşkun
    • Rüstem Nurten
  • View Affiliations / Copyright

    Affiliations: Department of Biophysics, Onsekiz Mart University Faculty of Medicine, Çanakkale 17100, Turkey, Department of Biophysics, Istanbul Faculty of Medicine, University of Istanbul, Çapa, Istanbul 34104, Turkey
  • Pages: 227-231
    |
    Published online on: May 8, 2013
       https://doi.org/10.3892/ol.2013.1335
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Abstract

In the present study, NAD+ glycohydrolase was purified from serum samples collected from healthy individuals using ammonium sulfate fractionation, Affi‑Gel blue (Cibacron Blue F3GA) affinity chromatography, Sephadex G‑100 column chromatography and isoelectric focusing. The final step was followed by a second Sephadex G‑100 column chromatography assay in order to remove the ampholytes from the isoelectric focusing step. In terms of enhancement of specific activity, the NAD+ glycohydrolase protein was purified ~480‑fold, with a yield of 1% compared with the initial serum fraction. The purified fraction appeared to be homogeneous, with a molecular weight of 39 kDa, as revealed by sodium dodecyl sulfate‑polyacrylamide gel electrophoresis (SDS‑PAGE) analysis, and also corresponded to the soluble (monomeric) form of surface antigen CD38.
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Copy and paste a formatted citation
Spandidos Publications style
Coşkun Ö and Nurten R: Purification of NAD+ glycohydrolase from human serum. Oncol Lett 6: 227-231, 2013.
APA
Coşkun, Ö., & Nurten, R. (2013). Purification of NAD+ glycohydrolase from human serum. Oncology Letters, 6, 227-231. https://doi.org/10.3892/ol.2013.1335
MLA
Coşkun, Ö., Nurten, R."Purification of NAD+ glycohydrolase from human serum". Oncology Letters 6.1 (2013): 227-231.
Chicago
Coşkun, Ö., Nurten, R."Purification of NAD+ glycohydrolase from human serum". Oncology Letters 6, no. 1 (2013): 227-231. https://doi.org/10.3892/ol.2013.1335
Copy and paste a formatted citation
x
Spandidos Publications style
Coşkun Ö and Nurten R: Purification of NAD+ glycohydrolase from human serum. Oncol Lett 6: 227-231, 2013.
APA
Coşkun, Ö., & Nurten, R. (2013). Purification of NAD+ glycohydrolase from human serum. Oncology Letters, 6, 227-231. https://doi.org/10.3892/ol.2013.1335
MLA
Coşkun, Ö., Nurten, R."Purification of NAD+ glycohydrolase from human serum". Oncology Letters 6.1 (2013): 227-231.
Chicago
Coşkun, Ö., Nurten, R."Purification of NAD+ glycohydrolase from human serum". Oncology Letters 6, no. 1 (2013): 227-231. https://doi.org/10.3892/ol.2013.1335
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