Telomerase reverse transcriptase promotes the proliferation of human laryngeal carcinoma cells through 
activation of the activator protein 1

Jiang,Yang; Chen,Chen; Chen,Shi-Ming; Wang,Ya-Qiu; Xu,Yong; Wang,Yan; Chen,Zhe; Xiao,Bo-Kui; Tao,Ze-Zhang

doi:10.3892/ol.2013.1344

July 2013 Volume 6 Issue 1

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July 2013 Volume 6 Issue 1

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Article

Telomerase reverse transcriptase promotes the proliferation of human laryngeal carcinoma cells through activation of the activator protein 1

Authors:
- Yang Jiang
- Chen Chen
- Shi-Ming Chen
- Ya-Qiu Wang
- Yong Xu
- Yan Wang
- Zhe Chen
- Bo-Kui Xiao
- Ze-Zhang Tao
View Affiliations / Copyright

Affiliations: Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, P.R. China
Pages: 75-80
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Published online on: May 8, 2013

https://doi.org/10.3892/ol.2013.1344
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Abstract

TERT is the main functional unit of telomerase, which maintains telomere length and chromosome structure stability. TERT has been shown to act as a key factor in various biological processes, such as cell proliferation, via uncharacterized mechanisms. We transfected HEp-2 laryngeal carcinoma cells with a TERT overexpressing adenovirus (Ad-TERT) and TERT shRNA silencing adenovirus (Ad-sh-TERT), and examined the effect on TERT and the AP-1 transcription factor subunits c-Fos and c-Jun using RT-PCR and western blot analysis. TERT mRNA expression was quantified using RT-PCR in 24 human laryngeal carcinoma samples, and TERT protein co-expression with AP-1 was investigated in a human laryngeal carcinoma tissue microarray using quantum-dot based immunofluorescence. The effect of specific ERK and p38 inhibitors on ERK, p38, c-Jun and c-Fos phosphorylation was investigated in TERT-overexpressing HEp-2 cells. TERT overexpression led to increased TERT, c-Jun and c-Fos mRNA and protein expression and increased cell proliferation, while TERT silencing had the opposite effects. TERT mRNA expression levels were positively correlated with c-Fos and c-Jun mRNA in human laryngeal carcinoma tissue. TERT and AP-1 protein were expressed at high levels and positively correlated in laryngeal carcinoma tissues. Treatment of TERT-overexpressing HEp-2 cells with specific p38 and ERK inhibitors indicated that TERT modulates the expression and phosphorylation of the AP-1 subunits c-Jun and c-Fos through the p38 and ERK signaling pathways. In conclusion, the results of this study indicate that TERT is capable of promoting cell proliferation via activation of the AP-1 subunits, c-Jun and c-Fos, in laryngeal carcinoma cells.

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