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Article

Characterization of p16 and E6 HPV‑related proteins in uterine cervix high‑grade lesions of patients treated by conization with large loop excision

  • Authors:
    • Maria Teresa Roncaglia
    • José Humberto T.G. Fregnani
    • Maricy Tacla
    • Silvana Gisele Pegorin De Campos
    • Hélio Hehl Caiaffa
    • Alexandre Ab'Saber
    • Eduardo Vieira Da Motta
    • Venâncio Avancini Ferreira Alves
    • Edmund C. Baracat
    • Adhemar Longatto Filho
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Faculty of Medicine, São Paulo University, Cerqueira César, São Paulo, Brazil , Center for Researcher Support, Barretos Cancer Hospital, Pio XII Foundation, Barretos, São Paulo, Brazil, Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, São Paulo, Brazil, Laboratory of Medical Investigation (LIM) 03, Clinics Hospital Faculty of Medicine, São Paulo University, Cerqueira César, São Paulo, Brazil , Department of Pathology, Clinics Hospital, São Paulo University, Cerqueira César, São Paulo, Brazil, Laboratory of Medical Investigation (LIM) 14, Department of Pathology, Faculty of Medicine, São Paulo University, Cerqueira César, São Paulo, Brazil
  • Pages: 63-68
    |
    Published online on: May 20, 2013
       https://doi.org/10.3892/ol.2013.1356
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Abstract

Cervical cancer and its precursor lesions represent a significant public health problem for developing and less‑developed countries. Cervical carcinogenesis is strongly correlated with persistent high‑risk human papillomavirus (HPV) infection, which is mostly associated with expression of the p16 and E6 HPV‑related proteins. The aim of this present study was to determine the expression of the p16 and E6 proteins in females with high‑grade lesions treated with conization, and to discuss the role of these proteins as prognostic markers following treatment. In total, 114 females were treated for high‑grade cervical intraepithelial neoplasia (CIN, grades 2/3) by conization with large loop excision of the transformation zone (LLETZ). Following surgery, the patients returned within 30‑45 days for post‑operative evaluation. A follow‑up was conducted every 6 months for 2 years. At each follow‑up appointment, a Pap smear, colposcopy and HPV DNA test were performed. E6 and p16 immunohistochemical tests were conducted on the surgical specimens. The positive expression of p16 was correlated with the presence of lesions with increased severity in the surgical specimens (P=0.0001). The expression of E6 did not demonstrate the same correlation (P=0.131). The HPV DNA hybrid, collected in the first post‑operative consultation as a predictor of the cytological abnormalities identified at the 24‑month follow‑up assessment, presented a sensitivity of 55.6%, a specificity of 84.8%, a positive predictive value of 33.3% and a negative predictive value of 93.3%. The role of p16INK4A as a marker of CIN was also demonstrated; the expression of p16 and E6, however, did not appear to be of any prognostic value in predicting the clearance of high‑risk HPV following conization. A negative hybrid capture test was correlated with a disease‑free outcome.
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1. 

zur Hausen H: Papillomaviruses causing cancer: evasion from host-cell control in early events in carcinogenesis. J Natl Cancer Inst. 92:690–698. 2000.PubMed/NCBI

2. 

zur Hausen H: Papillomaviruses and cancer: from basic studies to clinical application. Nat Rev Cancer. 2:342–350. 2002.PubMed/NCBI

3. 

Schiffman M, Castle PE, Jeronimo J, Rodriguez AC and Wacholder S: Human papillomavirus and cervical cancer. Lancet. 70:890–907. 2007. View Article : Google Scholar

4. 

Tungteakkhun SS and Duerksen-Hughes PJ: Cellular binding partners of the human papillomavirus E6 protein. Arch Virol. 153:397–408. 2008. View Article : Google Scholar : PubMed/NCBI

5. 

Doorbar J: The papillomavirus life cycle. J Clin Virol. 32(Suppl 1): S7–S15. 2005. View Article : Google Scholar

6. 

Cuschieri K and Wentzensen N: Human papillomavirus mRNA and p16 detection as biomarkers for the improved diagnosis of cervical neoplasia. Cancer Epidemiol Biomarkers Prev. 17:2536–2545. 2008. View Article : Google Scholar : PubMed/NCBI

7. 

Carozzi F, Confortini M, Dalla Palma P, Del Mistro A, Gillio-Tos A, De Marco L, et al: Use of p16-INK4A overexpression to increase the specificity of human papillomavirus testing: a nested substudy of the NTCC randomised controlled trial. Lancet Oncol. 9:937–945. 2008. View Article : Google Scholar : PubMed/NCBI

8. 

Lin HP, Wang YP and Chiang CP: Expression of p53, MDM2, p21, heat shock protein 70, and HPV 16/18 E6 proteins in oral verrucous carcinoma and oral verrucous hyperplasia. Head Neck. 33:334–340. 2011.PubMed/NCBI

9. 

Schiffman M, Wentzensen N, Wacholder S, Kinney W, Gage JC and Castle PE: Human papillomavirus testing in the prevention of cervical cancer. J Natl Cancer Inst. 103:368–383. 2011. View Article : Google Scholar : PubMed/NCBI

10. 

Longatto-Filho A, Etlinger D, Pereira SM, Kanamura CT, di Loreto C, Santos Gda C, et al: The association of p16(INK4A) and fragile histidine triad gene expression and cervical lesions. J Low Genit Tract Dis. 11:151–157. 2007. View Article : Google Scholar : PubMed/NCBI

11. 

Roncaglia MT, Tacla M, Vieira da Motta E, Caiaffa H, Ab’Saber A, Alves VA, Longatto Filho A and Baracat EC: Evaluation of the combination of cytology and hybrid capture to safely predict the high-grade lesion status of patients treated with conization with large loop excision of the transformation zone. Acta Cytol. 55:421–425. 2011. View Article : Google Scholar : PubMed/NCBI

12. 

Reuschenbach M, Clad A, von Knebel Doeberitz C, Wentzensen N, Rahmsdorf J, Schaffrath F, Griesser H, Freudenberg N and von Knebel Doeberitz M: Performance of p16INK4a-cytology, HPV mRNA, and HPV DNA testing to identify high grade cervical dysplasia in women with abnormal screening results. Gynecol Oncol. 119:98–105. 2010. View Article : Google Scholar : PubMed/NCBI

13. 

Vinokurova S and von Knebel Doeberitz M: Differential methylation of the HPV 16 upstream regulatory region during epithelial differentiation and neoplastic transformation. PLoS One. 6:e244512011. View Article : Google Scholar

14. 

Wu QJ, Guo M, Lu ZM, Li T, Qiao HZ and Ke Y: Detection of human papillomavirus-16 in ovarian malignancy. Br J Cancer. 89:672–675. 2003. View Article : Google Scholar : PubMed/NCBI

15. 

Qi ZL, Huo X, Xu XJ, Zhang B, Du MG, Yang HW, Zheng LK, Li J and Shen ZY: Relationship between HPV16/18 E6 and 53, 21WAF1, MDM2, Ki67 and cyclin D1 expression in esophageal squamous cell carcinoma: comparative study by using tissue microarray technology. Exp Oncol. 28:235–240. 2006.PubMed/NCBI

16. 

Lawson JS, Glenn WK, Heng B, Ye Y, Tran B, Lutze-Mann L and Whitaker NJ: Koilocytes indicate a role for human papilloma virus in breast cancer. Br J Cancer. 101:1351–1356. 2009. View Article : Google Scholar : PubMed/NCBI

17. 

Akil N, Yasmeen A, Kassab A, Ghabreau L, Darnel AD and Al Moustafa AE: High-risk human papillomavirus infections in breast cancer in Syrian women and their association with Id-1 expression: a tissue microarray study. Br J Cancer. 99:404–407. 2008. View Article : Google Scholar : PubMed/NCBI

18. 

Zehbe I, Lichtig H, Westerback A, Lambert PF, Tommasino M and Sherman L: Rare human papillomavirus 16 E6 variants reveal significant oncogenic potential. Mol Cancer. 10:772011. View Article : Google Scholar : PubMed/NCBI

19. 

Marks M, Castle PE, Schiffman M and Gravitt PE: Evaluation of any or type-specific persistence of high-risk human papillomavirus for detecting cervical precancer. J Clin Microbiol. 50:300–306. 2012. View Article : Google Scholar : PubMed/NCBI

20. 

Branca M, Ciotti M, Santini D, Di Bonito L, Giorgi C, Benedetto A, Paba P, Favalli C, Costa S, Agarossi A, Alderisio M and Syrjänen K: p16(INK4A) expression is related to grade of cin and high-risk human papillomavirus but does not predict virus clearance after conization or disease outcome. Int J Gynecol Pathol. 23:354–365. 2004. View Article : Google Scholar : PubMed/NCBI

21. 

Romagosa C, Simonetti S, López-Vicente L, Mazo A, Lleonart ME, Castellvi J and Ramon y Cajal S: p16(Ink4a) overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors. Oncogene. 30:2087–2097. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Roncaglia MT, Fregnani JH, Tacla M, De Campos SG, Caiaffa HH, Ab'Saber A, Da Motta EV, Alves VA, Baracat EC, Filho AL, Filho AL, et al: Characterization of p16 and E6 HPV‑related proteins in uterine cervix high‑grade lesions of patients treated by conization with large loop excision. Oncol Lett 6: 63-68, 2013.
APA
Roncaglia, M.T., Fregnani, J.H., Tacla, M., De Campos, S.G., Caiaffa, H.H., Ab'Saber, A. ... Filho, A.L. (2013). Characterization of p16 and E6 HPV‑related proteins in uterine cervix high‑grade lesions of patients treated by conization with large loop excision. Oncology Letters, 6, 63-68. https://doi.org/10.3892/ol.2013.1356
MLA
Roncaglia, M. T., Fregnani, J. H., Tacla, M., De Campos, S. G., Caiaffa, H. H., Ab'Saber, A., Da Motta, E. V., Alves, V. A., Baracat, E. C., Filho, A. L."Characterization of p16 and E6 HPV‑related proteins in uterine cervix high‑grade lesions of patients treated by conization with large loop excision". Oncology Letters 6.1 (2013): 63-68.
Chicago
Roncaglia, M. T., Fregnani, J. H., Tacla, M., De Campos, S. G., Caiaffa, H. H., Ab'Saber, A., Da Motta, E. V., Alves, V. A., Baracat, E. C., Filho, A. L."Characterization of p16 and E6 HPV‑related proteins in uterine cervix high‑grade lesions of patients treated by conization with large loop excision". Oncology Letters 6, no. 1 (2013): 63-68. https://doi.org/10.3892/ol.2013.1356
Copy and paste a formatted citation
x
Spandidos Publications style
Roncaglia MT, Fregnani JH, Tacla M, De Campos SG, Caiaffa HH, Ab'Saber A, Da Motta EV, Alves VA, Baracat EC, Filho AL, Filho AL, et al: Characterization of p16 and E6 HPV‑related proteins in uterine cervix high‑grade lesions of patients treated by conization with large loop excision. Oncol Lett 6: 63-68, 2013.
APA
Roncaglia, M.T., Fregnani, J.H., Tacla, M., De Campos, S.G., Caiaffa, H.H., Ab'Saber, A. ... Filho, A.L. (2013). Characterization of p16 and E6 HPV‑related proteins in uterine cervix high‑grade lesions of patients treated by conization with large loop excision. Oncology Letters, 6, 63-68. https://doi.org/10.3892/ol.2013.1356
MLA
Roncaglia, M. T., Fregnani, J. H., Tacla, M., De Campos, S. G., Caiaffa, H. H., Ab'Saber, A., Da Motta, E. V., Alves, V. A., Baracat, E. C., Filho, A. L."Characterization of p16 and E6 HPV‑related proteins in uterine cervix high‑grade lesions of patients treated by conization with large loop excision". Oncology Letters 6.1 (2013): 63-68.
Chicago
Roncaglia, M. T., Fregnani, J. H., Tacla, M., De Campos, S. G., Caiaffa, H. H., Ab'Saber, A., Da Motta, E. V., Alves, V. A., Baracat, E. C., Filho, A. L."Characterization of p16 and E6 HPV‑related proteins in uterine cervix high‑grade lesions of patients treated by conization with large loop excision". Oncology Letters 6, no. 1 (2013): 63-68. https://doi.org/10.3892/ol.2013.1356
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