Protective effect of tanshinone IIA against radiation‑induced ototoxicity in HEI-OC1 cells

Du,Shasha; Yao,Qiwei; Tan,Peixin; Xie,Guozhu; Ren,Chen; Sun,Quanquan; Zhang,Xiao; Zheng,Rong; Yang,Kaijun; Yuan,Yawei; Yuan,Quan

doi:10.3892/ol.2013.1486

October 2013 Volume 6 Issue 4

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

October 2013 Volume 6 Issue 4

Full Size Image

Article

Protective effect of tanshinone IIA against radiation‑induced ototoxicity in HEI-OC1 cells

Authors:
- Shasha Du
- Qiwei Yao
- Peixin Tan
- Guozhu Xie
- Chen Ren
- Quanquan Sun
- Xiao Zhang
- Rong Zheng
- Kaijun Yang
- Yawei Yuan
- Quan Yuan
View Affiliations / Copyright

Affiliations: Department of Radiation Oncology, Institute of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China, Department of Neurosurgery, Institute of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China, Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
Pages: 901-906
|
Published online on: July 24, 2013

https://doi.org/10.3892/ol.2013.1486
Expand metrics +

Abstract

Radiotherapy is a highly efficient treatment method for nasopharyngeal carcinoma that is often accompanied by significant ototoxic side‑effects. The inner ear hair cells are particularly prone to serious injury following radiotherapy. Tanshinone IIA is a transcription factor inhibitor that is extracted from the traditional herbal medicine, Salvia miltiorrhiza Bunge. The present study investigated the effects of tanshinone IIA treatment on radiation‑induced toxicity in the HEI‑OC1 hair cell line. Using an MTT assay and flow cytometry, the radiation‑induced weakening of the cells was observed to be alleviated when the cells were pre‑treated with tanshinone IIA. Radiation exposure promoted p65/nuclear factor (NF)‑κB nuclear translocation and activated the p53/p21 pathway, two processes which play a significant role in radiation‑induced cell apoptosis. However, pre‑treatment of the cells with tanshinone IIA inhibited p65/NF‑κB nuclear translocation and p53/p21 pathway activation. These results demonstrate that tanshinone IIA is capable of protecting cochlear cells from radiation‑induced injury through the suppression of p65/NF‑κB nuclear translocation and the p53/p21 signaling pathway.

View Figures

View References

1	Chan SH, Ng WT, Kam KL, et al: Sensorineural hearing loss after treatment of nasopharyngeal carcinoma: a longitudinal analysis. Int J Radiat Oncol Biol Phys. 73:1335–1342. 2009. View Article : Google Scholar : PubMed/NCBI
2	Petsuksiri J, Sermsree A, Thephamongkhol K, et al: Sensorineural hearing loss after concurrent chemoradiotherapy in nasopharyngeal cancer patients. Radiat Oncol. 6:192011. View Article : Google Scholar : PubMed/NCBI
3	Low WK, Burgess R, Fong KW and Wang DY: Effect of radiotherapy on retro-cochlear auditory pathways. Laryngoscope. 115:1823–1826. 2005. View Article : Google Scholar : PubMed/NCBI
4	Kelemen G: Radiation and ear. Experimental studies. Acta Otolaryngol. 184(Suppl 184): 1–48. 1963.PubMed/NCBI
5	Gamble JE, Peterson EA and Chandler JR: Radiation effects on the inner ear. Arch Otolaryngol. 88:156–161. 1968. View Article : Google Scholar : PubMed/NCBI
6	Low WK, Tan MG, Sun L, et al: Dose-dependent radiation-induced apoptosis in a cochlear cell-line. Apoptosis. 11:2127–2136. 2006. View Article : Google Scholar : PubMed/NCBI
7	Dong H, Mao S, Wei J, et al: Tanshinone IIA protects PC12 cells from β-amyloid(25–35)-induced apoptosis via PI3K/Akt signaling pathway. Mol Biol Rep. 39:6495–6503. 2012.
8	Zhu B, Zhai Q and Yu B: Tanshinone IIA protects rat primary hepatocytes against carbon tetrachloride toxicity via inhibiting mitochondria permeability transition. Pharm Biol. 48:484–487. 2010. View Article : Google Scholar
9	Xia WJ, Yang M, Fok TF, et al: Partial neuroprotective effect of pretreatment with tanshinone IIA on neonatal hypoxia-ischemia brain damage. Pediatr Res. 58:784–790. 2005. View Article : Google Scholar : PubMed/NCBI
10	Wang AM, Sha SH, Lesniak W and Schacht J: Tanshinone (Salviae miltiorrhizae extract) preparations attenuate aminoglycoside-induced free radical formation in vitro and ototoxicity in vivo. Antimicrob Agents Chemother. 47:1836–1841. 2003. View Article : Google Scholar
11	Dong X, Dong J, Zhang R, et al: Anti-inflammatory effects of tanshinone IIA on radiation-induced microglia BV-2 cells inflammatory response. Cancer Biother Radiopharm. 24:681–687. 2009. View Article : Google Scholar : PubMed/NCBI
12	Shinomiya N: New concepts in radiation-induced apoptosis: ‘premitotic apoptosis’ and ‘postmitotic apoptosis’. J Cell Mol Med. 5:240–253. 2001.
13	Löbrich M, Rief N, Kühne M, et al: In vivo formation and repair of DNA double-strand breaks after computed tomography examinations. Proc Natl Acad Sci USA. 102:8984–8989. 2005.PubMed/NCBI
14	Rübe CE, Dong X, Kühne M, et al: DNA double-strand break rejoining in complex normal tissues. Int J Radiat Oncol Biol Phys. 72:1180–1187. 2008.PubMed/NCBI
15	Hadian K and Krappmann D: Signals from the nucleus: activation of NF-kappaB by cytosolic ATM in the DNA damage response. Sci Signal. 4:pe22011.PubMed/NCBI
16	Wu ZH, Shi Y, Tibbetts RS and Miyamoto S: Molecular linkage between the kinase ATM and NF-kappaB signaling in response to genotoxic stimuli. Science. 311:1141–1146. 2006. View Article : Google Scholar : PubMed/NCBI
17	Grilli M and Memo M: Possible role of NF-kappaB and p53 in the glutamate-induced pro-apoptotic neuronal pathway. Cell Death Differ. 6:22–27. 1999. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Protective effect of tanshinone IIA against radiation‑induced ototoxicity in HEI-OC1 cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles