Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
October 2013 Volume 6 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October 2013 Volume 6 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Efficacy of 2‑(1‑hexyloxyethyl)‑2‑devinyl pyropheophorbide‑a in photodynamic therapy of human esophageal squamous cancer cells

  • Authors:
    • Dengpan Wu
    • Zhen Liu
    • Yanni Fu
    • Yuan Zhang
    • Nan Tang
    • Qin Wang
    • Liang Tao
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, Guangdong, Guangzhou 510080, P.R. China, Department of Anesthesiology, Sun Yat‑Sen Memorial Hospital, Sun Ya‑Sen University, Guangdong, Guangzhou 510120, P.R. China
  • Pages: 1111-1119
    |
    Published online on: July 25, 2013
       https://doi.org/10.3892/ol.2013.1493
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study investigated the effects of 2‑(1‑hexyloxyethyl)‑2‑devinylpyro pheophorbide‑a (HPPH)‑mediated photodynamic therapy (PDT) on in vitro cell survival and in vivo tumor growth derived from human esophageal squamous cancer cells (Eca109). A cell counting kit 8 (CCK8) assay was used to assess the phototoxicity of HPPH‑mediated PDT in cultured Eca109 cells. The inhibition of tumor growth was determined by the changes in the relative tumor volume (RTV) and tumor weight. The results revealed that HPPH, in the range of 0.005‑1 µg/ml, exhibited no cytotoxicity in the Eca109 cells without light exposure and that the in vitro efficiency of HPPH‑mediated PDT was higher compared with that of Photofrin®‑mediated PDT. The in vivo results indicated that graded doses of HPPH‑mediated PDT significantly inhibited the xenograft tumor growth derived from the Eca109 cells in a dose‑dependent manner. The inhibition efficacy of 0.6 and 1.0 mg/kg HPPH‑mediated PDT was similar to that of 10 mg/kg Photofrin‑mediated PDT. Furthermore, HPPH possessed a lower toxicity than Photofrin at the dose that achieved the same efficacy in mice bearing Eca109 subcutaneous tumors. The histopathological findings indicated that the tumor tissues in the photosensitizer (PS)‑treated mice demonstrated varying degrees of necrosis. HPPH and Photofrin exhibited vascular cytotoxicity on the treated tumors. In conclusion, the present study demonstrated that the phototoxicity of HPPH‑mediated PDT is higher than that of Photofrin‑mediated PDT of the same dose. HPPH possessed lower toxicity than Photofrin at the dose that achieved the same efficacy. Therefore, HPPH may be a promising agent for treating human esophageal squamous cell cancer (ESCC).
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Song C, Xing D, Tan W, Wei Q and Lin D: Methylenetetrahydrofolate reductase polymorphisms increase risk of esophageal squamous cell carcinoma in a Chinese population. Cancer Res. 61:3272–3275. 2001.PubMed/NCBI

2 

Fink U, Stein HJ and Siewert JR: Multimodal therapy of tumors of the upper gastrointestinal tract. Chirurg. 69:349–359. 1998.(In German).

3 

Siewert JR and Hölscher AH: Current strategy in surgery for esophageal cancer. Ann Ital Chir. 63:13–18. 1992.PubMed/NCBI

4 

Li SY, Sun XC and Liu L: Progress of medical and combined treatment for esophageal carcinoma. Ai Zheng. 25:509–515. 2006.(In Chinese).

5 

Dougherty TJ, Gomer CJ, Henderson BW, Jori G, Kessel D, Korbelik M, Moan J and Peng Q: Photodynamic therapy. J Natl Cancer Inst. 90:889–905. 1998. View Article : Google Scholar

6 

Webber J, Herman M, Kessel D and Fromm D: Current concepts in gastrointestinal photodynamic therapy. Ann Surg. 230:12–23. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Sibille A, Lambert R, Souquet JC, Sabben G and Descos F: Long-term survival after photodynamic therapy for esophageal cancer. Gastroenterology. 108:337–344. 1995. View Article : Google Scholar : PubMed/NCBI

8 

Allison RR, Downie GH, Cuenca R, Hu XH, Childs Carter JH and Sibata Claudio H: Photosensitizers in clinical PDT. Photodiagn Photodyn Ther. 1:27–42. 2004. View Article : Google Scholar

9 

Pandey RK, Bellnier DA, Smith KM and Dougherty TJ: Chlorin and porphyrin derivatives as potential photosensitizers in photodynamic therapy. Photochem Photobiol. 53:65–72. 1991. View Article : Google Scholar : PubMed/NCBI

10 

Henderson BW, Bellnier DA, Greco WR, Sharma A, Pandey RK, Vaughan LA, Weishaupt KR and Dougherty TJ: An in vivo quantitative structure-activity relationship for a congeneric series of pyropheophorbide derivatives as photosensitizers for photodynamic therapy. Cancer Res. 57:4000–4007. 1997.

11 

Lee LK, Whitehurst C, Pantelides ML and Moore JV: In situ comparison of 665 nm and 633 nm wavelength light penetration in the human prostate gland. Photochem Photobiol. 62:882–886. 1995. View Article : Google Scholar : PubMed/NCBI

12 

Bellnier DA, Henderson BW, Pandey RK, Potter WR and Dougherty TJ: Murine pharmacokinetics and antitumor efficacy of the photodynamic sensitizer 2-T1-hexyloxyethyl]-2-devinyl pyropheophorbide-a. J Photochem Photobiol B. 20:55–61. 1993.PubMed/NCBI

13 

Bellnier DA, Greco WR, Loewen GM, Nava H, Oseroff AR, Pandey RK, Tsuchida T and Dougherty TJ: Population pharmacokinetics of the photodynamic therapy agent 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-a in cancer patients. Cancer Res. 63:1806–1813. 2003.

14 

Srivatsan A, Ethirajan M, Pandey SK, Dubey S, Zheng X, Liu TH, Shibata M, Missert J, Morgan J and Pandey RK: Conjugation of cRGD peptide to chlorophyll a based photosensitizer (HPPH) alters its pharmacokinetics with enhanced tumor-imaging and photosensitizing (PDT) efficacy. Mol Pharm. 8:1186–1197. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Fingar VH, Wieman TJ and Haydon PS: The effects of thrombocytopenia on vessel stasis and macromolecular leakage after photodynamic therapy using photofrin. Photochem Photobiol. 66:513–517. 1997. View Article : Google Scholar : PubMed/NCBI

16 

Sancéau J, Poupon MF, Delattre O, Sastre-Garau X and Wietzerbin J: Strong inhibition of Ewing tumor xenograft growth by combination of human interferon-alpha or interferon-beta with ifosfamide. Oncogene. 21:7700–7709. 2002.

17 

Bissery MC, Guenard D, Guéritte-Voegelein F and Lavelle F: Experimental antitumor activity of taxotere (RP 56976, NSC 628503), a taxol analogue. Cancer Res. 51:4845–4852. 1991.PubMed/NCBI

18 

Hajri A, Wack S, Meyer C, Smith MK, Leberquier C, Kedinger M and Aprahamian M: In vitro and in vivo efficacy of photofrint and pheophorbide a, a bacteriochlorin, in photodynamic therapy of colonic cancer cells. Photochem Photobiol. 75:140–148. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Zheng X, Morgan J, Pandey SK, Chen Y, Tracy E, Baumann H, Missert JR, Batt C, Jackson J, Bellnier DA, Henderson BW and Pandey RK: Conjugation of 2-(1′-hexyloxyethyl)-2-devinylpyropheophorbide-a (HPPH) to carbohydrates changes its subcellular distribution and enhances photodynamic activity in vivo. J Med Chem. 52:4306–4318. 2009.

20 

Gomer CJ, Ferrario A, Hayashi N, Rucker N, Szirth BC and Murphree AL: Molecular, cellular, and tissue responses following photodynamic therapy. Lasers Surg Med. 8:450–463. 1988. View Article : Google Scholar : PubMed/NCBI

21 

Moor AC: Signaling pathways in cell death and survival after photodynamic therapy. J Photochem Photobiol B. 57:1–13. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Foster TH, Murant RS, Bryant RG, Knox RS, Gibson SL and Hilf R: Oxygen consumption and diffusion effects in photodynamic therapy. Radiat Res. 126:296–303. 1991. View Article : Google Scholar : PubMed/NCBI

23 

Wang KK, Mitra S and Foster TH: A comprehensive mathematical model of microscopic dose deposition in photodynamic therapy. Med Phys. 34:282–293. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Furukawa K, Yamamoto H, Crean DH, Kato H and Mang TS: Localization and treatment of transformed tissues using the photodynamic sensitizer 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-a. Laser Surg Med. 18:157–166. 1996.PubMed/NCBI

25 

Kawazoe K, Isomoto H, Yamaguchi N, Inoue N, Uehara R, Matsushima K, Ichikawa T, Takeshima F, Nonaka T, Nanashima A, Nagayasu T, Uehara M, Asahina I and Nakao K: Effects of photodynamic therapy for superficial esophageal squamous cell carcinoma in vivo and in vitro. Oncol Lett. 1:877–882. 2010.PubMed/NCBI

26 

Yang PW, Hung MC, Hsieh CY, Tung EC, Wang YH, Tsai JC and Lee JM: The effects of Photofrin-mediated photodynamic therapy on the modulation of EGFR in esophageal squamous cell carcinoma cells. Laser Med Sci. 28:605–614. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Lobel J, MacDonald IJ, Ciesielski MJ, Barone T, Potter WR, Pollina J, Plunkett RJ, Fenstermaker RA and Dougherty TJ: 2-[1-Hexyloxyethyl]-2-Devinyl Pyropheophorbide-a (HPPH) in a nude rat glioma model: Implications for photodynamic therapy. Laser Surg Med. 29:397–405. 2001.

28 

Ferrario A and Gomer CJ: Systemic toxicity in mice induced by localized porphyrin photodynamic therapy. Cancer Res. 50:539–543. 1990.PubMed/NCBI

29 

Dougherty TJ: Photosensitizers: therapy and detection of malignant tumors. Photochem Photobiol. 45:879–889. 1987. View Article : Google Scholar : PubMed/NCBI

30 

Huang Z, Chen Q, Shakil A, Chen H, Beckers J, Shapiro H and Hetzel FW: Hyperoxygenation enhances the tumor cell killing of photofrin-mediated photodynamic therapy. Photochem Photobiol. 78:496–502. 2003. View Article : Google Scholar : PubMed/NCBI

31 

Henderson BW, Waldow SM, Mang TS, Potter WR, Malone PB and Dougherty TJ: Tumor destruction and kinetics of tumor cell death in two experimental mouse tumors following photodynamic therapy. Cancer Res. 45:572–576. 1985.PubMed/NCBI

32 

Henderson BW and Fingar VH: Oxygen limitation of direct tumor cell kill during photodynamic treatment of a murine tumor model. Photochem Photobiol. 49:299–304. 1989. View Article : Google Scholar : PubMed/NCBI

33 

Snyder JW, Greco WR, Bellnier DA, Vaughan L and Henderson BW: Photodynamic therapy: a means to enhanced drug delivery to tumors. Cancer Res. 63:8126–8131. 2003.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wu D, Liu Z, Fu Y, Zhang Y, Tang N, Wang Q and Tao L: Efficacy of 2‑(1‑hexyloxyethyl)‑2‑devinyl pyropheophorbide‑a in photodynamic therapy of human esophageal squamous cancer cells. Oncol Lett 6: 1111-1119, 2013.
APA
Wu, D., Liu, Z., Fu, Y., Zhang, Y., Tang, N., Wang, Q., & Tao, L. (2013). Efficacy of 2‑(1‑hexyloxyethyl)‑2‑devinyl pyropheophorbide‑a in photodynamic therapy of human esophageal squamous cancer cells. Oncology Letters, 6, 1111-1119. https://doi.org/10.3892/ol.2013.1493
MLA
Wu, D., Liu, Z., Fu, Y., Zhang, Y., Tang, N., Wang, Q., Tao, L."Efficacy of 2‑(1‑hexyloxyethyl)‑2‑devinyl pyropheophorbide‑a in photodynamic therapy of human esophageal squamous cancer cells". Oncology Letters 6.4 (2013): 1111-1119.
Chicago
Wu, D., Liu, Z., Fu, Y., Zhang, Y., Tang, N., Wang, Q., Tao, L."Efficacy of 2‑(1‑hexyloxyethyl)‑2‑devinyl pyropheophorbide‑a in photodynamic therapy of human esophageal squamous cancer cells". Oncology Letters 6, no. 4 (2013): 1111-1119. https://doi.org/10.3892/ol.2013.1493
Copy and paste a formatted citation
x
Spandidos Publications style
Wu D, Liu Z, Fu Y, Zhang Y, Tang N, Wang Q and Tao L: Efficacy of 2‑(1‑hexyloxyethyl)‑2‑devinyl pyropheophorbide‑a in photodynamic therapy of human esophageal squamous cancer cells. Oncol Lett 6: 1111-1119, 2013.
APA
Wu, D., Liu, Z., Fu, Y., Zhang, Y., Tang, N., Wang, Q., & Tao, L. (2013). Efficacy of 2‑(1‑hexyloxyethyl)‑2‑devinyl pyropheophorbide‑a in photodynamic therapy of human esophageal squamous cancer cells. Oncology Letters, 6, 1111-1119. https://doi.org/10.3892/ol.2013.1493
MLA
Wu, D., Liu, Z., Fu, Y., Zhang, Y., Tang, N., Wang, Q., Tao, L."Efficacy of 2‑(1‑hexyloxyethyl)‑2‑devinyl pyropheophorbide‑a in photodynamic therapy of human esophageal squamous cancer cells". Oncology Letters 6.4 (2013): 1111-1119.
Chicago
Wu, D., Liu, Z., Fu, Y., Zhang, Y., Tang, N., Wang, Q., Tao, L."Efficacy of 2‑(1‑hexyloxyethyl)‑2‑devinyl pyropheophorbide‑a in photodynamic therapy of human esophageal squamous cancer cells". Oncology Letters 6, no. 4 (2013): 1111-1119. https://doi.org/10.3892/ol.2013.1493
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team