Inhibition of nm23‑H1 gene expression in chronic myelogenous leukemia cells

Dai,Zhensheng; Xiao,Weizhong; Jin,Yueling

doi:10.3892/ol.2013.1500

Inhibition of nm23‑H1 gene expression in chronic myelogenous leukemia cells

Authors:
- Zhensheng Dai
- Weizhong Xiao
- Yueling Jin
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Affiliations: Department of Hematology, Shanghai Pudong Hospital, Affiliated to Fudan University, Shanghai 201399, P.R. China, Department of Neurology, Shanghai Pudong Hospital, Affiliated to Fudan University, Shanghai 201399, P.R. China, Department of Pathology, Shanghai Health Vocational Technical College, Xuhui, Shanghai 200237, P.R. China
Published online on: July 29, 2013 https://doi.org/10.3892/ol.2013.1500
Pages: 1093-1097

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Abstract

For solid tumors of a malignant origin, the expression of the nm23‑H1 gene is a positive prognostic factor. However, for chronic myeloid leukemia (CML), the prognostic role of nm23‑H1 gene expression is unknown. The present study investigated the impact of nm23‑H1 gene expression on the proliferation and migration of the CML K562 cell line to elucidate the association between nm23‑H1 gene expression and CML cell survival. An RNAi lipo‑recombinant plasmid of the nm23‑H1 gene (pGCsi‑nm23‑H1) was constructed and transfected into the K562 cells. RT‑PCR and western blotting were used to detect nm23‑H1 mRNA and protein expression, respectively. The anchorage‑independent growth ability of the transfected cells was observed in soft agar culture and the ability of the K562 cells to migrate was determined using a Transwell assay. Following the successful construction and transfection of the pGCsi‑nm23‑H1 plasmid into the K562 cells, nm23‑H1 mRNA and protein expression levels were significantly lower compared with the control group. The stably‑transfected pGCsi‑nm23‑H1 K562 cells exhibited a markedly increased ability to form colonies and the number and sizes of the colonies were significantly increased compared with those of the control. In vitro, the cells migrated through a Matrigel‑coated membrane during incubation for 20 h. The Transwell assay revealed that the quantitative number of pGCsi‑nm23‑H1 K562 cells that migrated into the lower compartment of the invasion chamber was markedly increased compared with the control. In conclusion, nm23‑H1 gene expression may inhibit K562 cell proliferation and migration. nm23‑H1 may be a cancer suppressor gene and play a significant role in inhibiting the survival of CML cells.

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