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Article

Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle

  • Authors:
    • Wei Lin
    • Liangpu Zheng
    • Qunchuan Zhuang
    • Aling Shen
    • Liya Liu
    • Youqin Chen
    • Thomas J. Sferra
    • Jun Peng
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Rainbow Babies and Children's Hospital, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
  • Pages: 1123-1127
    |
    Published online on: August 5, 2013
       https://doi.org/10.3892/ol.2013.1512
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Abstract

Spica Prunellae has long been used as a significant component in numerous traditional Chinese medicine (TCM) formulas to clinically treat cancers. Previously, Spica Prunellae was shown to promote cancer cell apoptosis and inhibit angiogenesis in vivo and in vitro. To further elucidate the precise mechanism of its tumoricidal activity, the effect of the ethanol extract of Spica Prunellae (EESP) on the proliferation of human colon carcinoma HT‑29 cells was elucidated and the underlying molecular mechanisms were investigated. The proliferation of HT‑29 cells was evaluated using 3‑(4, 5‑dimethyl‑thiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) and colony formation analyses. The cell cycle was determined using fluorescence‑activated cell sorting (FACS) with propidium iodide (PI) staining. The mRNA and protein expression of cyclin‑dependent kinase 4 (CDK4) and cyclin D1 was examined using RT‑PCR and western blotting, respectively. EESP was observed to inhibit HT‑29 viability and survival in a dose‑ and time‑dependent manner. Furthermore, EESP treatment blocked G1/S cell cycle progression and reduced the expression of pro‑proliferative cyclin D1 and CDK4 at the transcriptional and translational levels. Altogether, these data suggest that the inhibition of cell proliferation via G1/S cell cycle arrest may be one of the mechanisms through which Spica Prunellae treats cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Lin W, Zheng L, Zhuang Q, Shen A, Liu L, Chen Y, Sferra TJ and Peng J: Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle. Oncol Lett 6: 1123-1127, 2013.
APA
Lin, W., Zheng, L., Zhuang, Q., Shen, A., Liu, L., Chen, Y. ... Peng, J. (2013). Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle. Oncology Letters, 6, 1123-1127. https://doi.org/10.3892/ol.2013.1512
MLA
Lin, W., Zheng, L., Zhuang, Q., Shen, A., Liu, L., Chen, Y., Sferra, T. J., Peng, J."Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle". Oncology Letters 6.4 (2013): 1123-1127.
Chicago
Lin, W., Zheng, L., Zhuang, Q., Shen, A., Liu, L., Chen, Y., Sferra, T. J., Peng, J."Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle". Oncology Letters 6, no. 4 (2013): 1123-1127. https://doi.org/10.3892/ol.2013.1512
Copy and paste a formatted citation
x
Spandidos Publications style
Lin W, Zheng L, Zhuang Q, Shen A, Liu L, Chen Y, Sferra TJ and Peng J: Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle. Oncol Lett 6: 1123-1127, 2013.
APA
Lin, W., Zheng, L., Zhuang, Q., Shen, A., Liu, L., Chen, Y. ... Peng, J. (2013). Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle. Oncology Letters, 6, 1123-1127. https://doi.org/10.3892/ol.2013.1512
MLA
Lin, W., Zheng, L., Zhuang, Q., Shen, A., Liu, L., Chen, Y., Sferra, T. J., Peng, J."Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle". Oncology Letters 6.4 (2013): 1123-1127.
Chicago
Lin, W., Zheng, L., Zhuang, Q., Shen, A., Liu, L., Chen, Y., Sferra, T. J., Peng, J."Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle". Oncology Letters 6, no. 4 (2013): 1123-1127. https://doi.org/10.3892/ol.2013.1512
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