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Article

Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia

  • Authors:
    • Xu Chen
    • Hui Zhang
    • Ping Li
    • Zheng Yang
    • Lingyan Qin
    • Wuning Mo
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
  • Pages: 963-969
    |
    Published online on: August 5, 2013
       https://doi.org/10.3892/ol.2013.1514
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Abstract

The aim of the present study was to analyze the expression of WW-domain oxidoreductase (WWOX), fragile histidine triad (FHIT) and p73 in acute lymphoblastic leukemia (ALL). Samples from 122 ALL patients and 35 non-ALL control patients were collected in this study. RT‑PCR was performed to detect the mRNA expression of WWOX, FHIT and p73. The methylation status of the WWOX promoter region, FHIT promoter region and the first exon region of p73 were also analyzed using the methylation-specific PCR method. The mRNA expression of WWOX, FHIT and p73 was significantly lower in the ALL samples compared with the controls (48.2, 42.9 and 55.4%, respectively). By contrast, the methylation frequency of WWOX, FHIT and p73 was significantly higher in the ALL samples compared with the controls (44.6, 46.4 and 37.5%, respectively). The mRNA expression of these three genes was inversely correlated with the methylation frequency in the ALL samples (correlation coefficients, -0.661, -0.685 and -0.536 for WWOX, FHIT and p73, respectively). Moreover, the mRNA expression of WWOX was positively correlated with that of FHIT and p73 (correlation coefficients, 0.569 and 0.556, respectively). However, the methylation status of WWOX had no correlation with that of FHIT or p73. It was concluded that the high methylation status of WWOX, FHIT and p73 may lead to the inactivation of expression and the silencing of these genes, promoting the occurrence and development of ALL. The determination of the mRNA expression and methylation status of WWOX, FHIT and p73 may aid in the development of treatment approaches for ALL.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Zhang H, Li P, Yang Z, Qin L and Mo W: Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia. Oncol Lett 6: 963-969, 2013.
APA
Chen, X., Zhang, H., Li, P., Yang, Z., Qin, L., & Mo, W. (2013). Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia. Oncology Letters, 6, 963-969. https://doi.org/10.3892/ol.2013.1514
MLA
Chen, X., Zhang, H., Li, P., Yang, Z., Qin, L., Mo, W."Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia". Oncology Letters 6.4 (2013): 963-969.
Chicago
Chen, X., Zhang, H., Li, P., Yang, Z., Qin, L., Mo, W."Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia". Oncology Letters 6, no. 4 (2013): 963-969. https://doi.org/10.3892/ol.2013.1514
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Zhang H, Li P, Yang Z, Qin L and Mo W: Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia. Oncol Lett 6: 963-969, 2013.
APA
Chen, X., Zhang, H., Li, P., Yang, Z., Qin, L., & Mo, W. (2013). Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia. Oncology Letters, 6, 963-969. https://doi.org/10.3892/ol.2013.1514
MLA
Chen, X., Zhang, H., Li, P., Yang, Z., Qin, L., Mo, W."Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia". Oncology Letters 6.4 (2013): 963-969.
Chicago
Chen, X., Zhang, H., Li, P., Yang, Z., Qin, L., Mo, W."Gene expression of WWOX, FHIT and p73 in acute lymphoblastic leukemia". Oncology Letters 6, no. 4 (2013): 963-969. https://doi.org/10.3892/ol.2013.1514
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