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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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Article

β4‑integrin‑mediated cytotoxic activity of AexU in human prostate cancer PC3 cells

  • Authors:
    • Masafumi Kumano
    • Hideaki Miyake
    • Said K. Abolghait
    • Hosny M. Behnsawy
    • Masato Fujisawa
  • View Affiliations / Copyright

    Affiliations: Division of Urology, Kobe University Graduate School of Medicine, Kobe, Japan, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
  • Pages: 1482-1486
    |
    Published online on: August 23, 2013
       https://doi.org/10.3892/ol.2013.1542
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Abstract

The present study aimed to characterize the cytotoxic activity of AexU, an effector‑mediating type three secretion system (TTSS) of gram‑negative bacteria, in human prostate cancer cells, focusing on the association with β4‑integrin expression. The cytotoxic effects of AexU either alone or in combination with chemotherapeutic agents were evaluated using several human prostate cancer cell lines. Human prostate cancer PC3 cells, in which an expression vector containing siRNA targeting β4‑integrin had been introduced, were established (PC3/sh‑In), and the cytotoxic effects of AexU on the PC3/sh‑In cells were compared with the PC3 cells that were transfected with a control vector (PC3/C). The expression levels of β4‑integrin in the PC3 cells were markedly higher compared with those in the LNCaP or DU145 cells, and the cytotoxic effects of AexU in the PC3 cells were more pronounced compared with those in the LNCaP or DU145 cells. The sensitivity of the PC3 cells to docetaxel and cisplatin was significantly enhanced following treatment with AexU, resulting in a decrease in the IC50 of the two agents by ~90%. The cytotoxic effect of AexU in the PC3/C cells was more marked compared with that in the PC3/sh‑In cells, and the phosphorylation of Akt in the PC3/C cells appeared to be significantly more inhibited by the treatment with AexU compared with the PC3/sh‑In cells. In conclusion, treatment with AexU may be a useful therapeutic option for prostate cancer when β4‑integrin is overexpressed. The treatment appears to exert its effects through growth inhibition and by enhancing the sensitivity of the cancer cells to chemotherapeutic agents.
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Copy and paste a formatted citation
Spandidos Publications style
Kumano M, Miyake H, Abolghait SK, Behnsawy HM and Fujisawa M: β4‑integrin‑mediated cytotoxic activity of AexU in human prostate cancer PC3 cells. Oncol Lett 6: 1482-1486, 2013.
APA
Kumano, M., Miyake, H., Abolghait, S.K., Behnsawy, H.M., & Fujisawa, M. (2013). β4‑integrin‑mediated cytotoxic activity of AexU in human prostate cancer PC3 cells. Oncology Letters, 6, 1482-1486. https://doi.org/10.3892/ol.2013.1542
MLA
Kumano, M., Miyake, H., Abolghait, S. K., Behnsawy, H. M., Fujisawa, M."β4‑integrin‑mediated cytotoxic activity of AexU in human prostate cancer PC3 cells". Oncology Letters 6.5 (2013): 1482-1486.
Chicago
Kumano, M., Miyake, H., Abolghait, S. K., Behnsawy, H. M., Fujisawa, M."β4‑integrin‑mediated cytotoxic activity of AexU in human prostate cancer PC3 cells". Oncology Letters 6, no. 5 (2013): 1482-1486. https://doi.org/10.3892/ol.2013.1542
Copy and paste a formatted citation
x
Spandidos Publications style
Kumano M, Miyake H, Abolghait SK, Behnsawy HM and Fujisawa M: β4‑integrin‑mediated cytotoxic activity of AexU in human prostate cancer PC3 cells. Oncol Lett 6: 1482-1486, 2013.
APA
Kumano, M., Miyake, H., Abolghait, S.K., Behnsawy, H.M., & Fujisawa, M. (2013). β4‑integrin‑mediated cytotoxic activity of AexU in human prostate cancer PC3 cells. Oncology Letters, 6, 1482-1486. https://doi.org/10.3892/ol.2013.1542
MLA
Kumano, M., Miyake, H., Abolghait, S. K., Behnsawy, H. M., Fujisawa, M."β4‑integrin‑mediated cytotoxic activity of AexU in human prostate cancer PC3 cells". Oncology Letters 6.5 (2013): 1482-1486.
Chicago
Kumano, M., Miyake, H., Abolghait, S. K., Behnsawy, H. M., Fujisawa, M."β4‑integrin‑mediated cytotoxic activity of AexU in human prostate cancer PC3 cells". Oncology Letters 6, no. 5 (2013): 1482-1486. https://doi.org/10.3892/ol.2013.1542
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