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November 2013 Volume 6 Issue 5

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway

  • Authors:
    • Guang‑Ning Yan
    • Yang-Fan Lv
    • Lang Yang
    • Xiao‑Hong Yao
    • You‑Hong Cui
    • De‑Yu Guo
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Southwest Hospital, Third Military Medical University of PLA, Shapingba District, Chongqing 400038, P.R. China
  • Pages: 1524-1530
    |
    Published online on: September 9, 2013
       https://doi.org/10.3892/ol.2013.1569
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Abstract

The aim of the present study was to determine the possible mechanism underlying the enhanced migration and proliferation of endothelial cells caused by glioma stem cells (GSCs). Tumor spheres enriched in GSCs derived from the mouse GL261 glioma cell line, and the brain microvessel endothelial cell line, b.END3, were used in this study. A Transwell co‑culture system, RNAi experiments, quantitative polymerase chain reaction, western blotting and enzyme‑linked immunosorbent, cell counting kit‑8 (CCK‑8) proliferation, Transwell migration and wound‑healing assays were used in this study to determine the migration and proliferation ability, as well as the Hedgehog (HH) pathway‑related gene expression in the b.END3 cells. Based on the results, it was demonstrated that the migration and proliferation of the endothelial cells were enhanced following co‑culture with GSCs. The gene expression of the HH pathway‑related genes, Sonic Hedgehog (Shh) and Hedgehog‑interacting protein (Hhip) was altered in the endothelial cells when co‑cultured with GSCs. Overexpression of glioma‑associated oncogene homolog 1 indicated activation of the HH pathway. Following knockdown of smoothened (Smo) in the endothelial cells, the migration and proliferation abilities of the cells were inhibited. GSCs have little effect on enhancing these behaviors in endothelial cells following Smo‑knockdown. Further investigation revealed that Shh levels in the supernatant of the co‑culture system were elevated, indicating the importance of secreted Shh from the endothelial cells. In conclusion, GSCs enhanced the migration and proliferation of the endothelial cells in vitro, which was likely associated with the activation of the HH pathway in the endothelial cells, caused by the increased secretion of Shh.
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Copy and paste a formatted citation
Spandidos Publications style
Yan GN, Lv Y, Yang L, Yao XH, Cui YH and Guo DY: Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway. Oncol Lett 6: 1524-1530, 2013.
APA
Yan, G., Lv, Y., Yang, L., Yao, X., Cui, Y., & Guo, D. (2013). Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway. Oncology Letters, 6, 1524-1530. https://doi.org/10.3892/ol.2013.1569
MLA
Yan, G., Lv, Y., Yang, L., Yao, X., Cui, Y., Guo, D."Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway". Oncology Letters 6.5 (2013): 1524-1530.
Chicago
Yan, G., Lv, Y., Yang, L., Yao, X., Cui, Y., Guo, D."Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway". Oncology Letters 6, no. 5 (2013): 1524-1530. https://doi.org/10.3892/ol.2013.1569
Copy and paste a formatted citation
x
Spandidos Publications style
Yan GN, Lv Y, Yang L, Yao XH, Cui YH and Guo DY: Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway. Oncol Lett 6: 1524-1530, 2013.
APA
Yan, G., Lv, Y., Yang, L., Yao, X., Cui, Y., & Guo, D. (2013). Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway. Oncology Letters, 6, 1524-1530. https://doi.org/10.3892/ol.2013.1569
MLA
Yan, G., Lv, Y., Yang, L., Yao, X., Cui, Y., Guo, D."Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway". Oncology Letters 6.5 (2013): 1524-1530.
Chicago
Yan, G., Lv, Y., Yang, L., Yao, X., Cui, Y., Guo, D."Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway". Oncology Letters 6, no. 5 (2013): 1524-1530. https://doi.org/10.3892/ol.2013.1569
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