Differential effects of miR‑34c‑3p and miR‑34c‑5p on the proliferation, apoptosis and invasion of glioma cells

Wu,Zhendong; Wu,Yupeng; Tian,Ye; Sun,Xiaofeng; Liu,Jixiang; Ren,Hongbo; Liang,Chaohui; Song,Ligang; Hu,Hongchao; Wang,Liqun; Jiao,Baohua

doi:10.3892/ol.2013.1579

Differential effects of miR‑34c‑3p and miR‑34c‑5p on the proliferation, apoptosis and invasion of glioma cells

Authors:
- Zhendong Wu
- Yupeng Wu
- Ye Tian
- Xiaofeng Sun
- Jixiang Liu
- Hongbo Ren
- Chaohui Liang
- Ligang Song
- Hongchao Hu
- Liqun Wang
- Baohua Jiao
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Affiliations: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shi Jiazhuang, Hebei 050000, P.R. China, Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Department of Neurosurgery, Handan Central Hospital, Handan, Hebei 056000, P.R. China
Published online on: September 12, 2013 https://doi.org/10.3892/ol.2013.1579
Pages: 1447-1452

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Abstract

Glioblastoma is the most malignant and common intrinsic brain tumor, but the molecular mechanism of glioma pathophysiology is poorly understood. Recent data have shown that microRNAs regulate the expression of several genes associated with human cancer. In the present study, the function of miR‑34c in glioma cells was analyzed. It was demonstrated that miR‑34c‑3p and miR‑34c‑5p were downregulated in gliomas, by performing qPCR on tumor tissues from glioma patients and glioma cell lines, compared with normal brain tissues and a normal glial cell line. Furthermore, the miR‑34c expression was found to be inversely correlated with glioma WHO grades. Overexpression of miR‑34c‑3p inhibited U251 and U87 cell proliferation; however, miR‑34c‑5p only had an effect on U251 cells. Transfection with miR‑34c‑3p or miR‑34c‑5p in U251 cells and with miR‑34c‑3p in U87 cells produced S‑phase arrest with G0/G1 reduction and induced cell apoptosis, but no significant changes were observed with miR‑34c‑5p transfection in U87 cells, normal or negative control groups. However, significant inhibition of glioma cell invasion was observed following transfection with miR‑34c‑3p and miR‑34c‑5p. Moreover, it was identified that miR‑34c‑3p overexpression reduced the expression of Notch pathway members, but miR‑34c‑5p overexpression did not. Therefore, these results suggest differential tumor suppressor roles for miR‑34c‑3p and miR‑34c‑5p and provide new insights into the role of miR‑34c in glioma, which includes tumor‑suppressing effects on proliferation, apoptosis and invasiveness.

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