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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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2014-January Volume 7 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro

  • Authors:
    • Jianfeng Zhang
    • Xiaoling Kuai
    • Mengjiao Song
    • Xiaoqi  Chen
    • Zhihua Yu
    • Hong Zhang
    • Zhenbiao Mao
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
  • Pages: 270-274
    |
    Published online on: November 7, 2013
       https://doi.org/10.3892/ol.2013.1667
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Abstract

microRNAs (miRNAs) are a class of endogenously expressed, small non‑coding RNAs, which suppress their target mRNAs at the post‑transcriptional level. miRNAs play key roles in tumor metastasis. The aim of the present study was to investigate the expression of miRNA‑32 (miR‑32) on the biological behavior of the human gastric cancer cell line, SGC‑7901. SGC‑7901 cells were transfected with miR‑32‑mimic, miR‑32‑inhibitor and empty plasmid vectors using Lipofectamine™ 2000. The expression of GFP was observed by fluorescent microscopy and miR‑32 gene expression was detected by quantitative polymerase chain reaction. The cell counting kit‑8 assay was performed to evaluate the effect of miR‑32 expression on cell proliferation in vitro. Alterations in the migration and metastatic potential of SGC‑7901 cells, prior to and following miR‑32 gene transfection, were assayed by cell chemotactic migration and invasion tests. The results of the current study showed that the proliferation rate of the transfected SGC‑7901 cells overexpressing miR‑32 is reduced and cell chemotactic migration and invasion potentials is markedly reduced following miR‑32‑mimic transfection (P<0.05). In addition, the results demonstrated that overexpression of miR‑32 greatly inhibits the proliferation and decreases the migration and invasion capabilities of SGC‑7901 cells in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Kuai X, Song M, Chen X, Yu Z, Zhang H and Mao Z: microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro. Oncol Lett 7: 270-274, 2014.
APA
Zhang, J., Kuai, X., Song, M., Chen, X., Yu, Z., Zhang, H., & Mao, Z. (2014). microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro. Oncology Letters, 7, 270-274. https://doi.org/10.3892/ol.2013.1667
MLA
Zhang, J., Kuai, X., Song, M., Chen, X., Yu, Z., Zhang, H., Mao, Z."microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro". Oncology Letters 7.1 (2014): 270-274.
Chicago
Zhang, J., Kuai, X., Song, M., Chen, X., Yu, Z., Zhang, H., Mao, Z."microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro". Oncology Letters 7, no. 1 (2014): 270-274. https://doi.org/10.3892/ol.2013.1667
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Kuai X, Song M, Chen X, Yu Z, Zhang H and Mao Z: microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro. Oncol Lett 7: 270-274, 2014.
APA
Zhang, J., Kuai, X., Song, M., Chen, X., Yu, Z., Zhang, H., & Mao, Z. (2014). microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro. Oncology Letters, 7, 270-274. https://doi.org/10.3892/ol.2013.1667
MLA
Zhang, J., Kuai, X., Song, M., Chen, X., Yu, Z., Zhang, H., Mao, Z."microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro". Oncology Letters 7.1 (2014): 270-274.
Chicago
Zhang, J., Kuai, X., Song, M., Chen, X., Yu, Z., Zhang, H., Mao, Z."microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro". Oncology Letters 7, no. 1 (2014): 270-274. https://doi.org/10.3892/ol.2013.1667
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