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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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2014-January Volume 7 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Frizzled‑2: A potential novel target for molecular pancreatic cancer therapy

  • Authors:
    • Minoru Tomizawa
    • Fuminobu Shinozaki
    • Takao Sugiyama
    • Shigenori  Yamamoto
    • Makoto Sueishi
    • Takanobu Yoshida
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Radiology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Rheumatology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Pediatrics, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan, Department of Internal Medicine, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284‑0003, Japan
  • Pages: 74-78
    |
    Published online on: November 12, 2013
       https://doi.org/10.3892/ol.2013.1681
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Abstract

In the present study, pancreatic cancer cell proliferation was analyzed following the suppression of frizzled (Fz)2 expression. Reverse transcription polymerase chain reaction (PCR) was performed using RNA isolated from pancreatic cancer cell lines, PANC‑1, NOR‑P1, PK‑45H, PK‑1, PK‑59, MIA‑Paca2 and KP4. A surgical specimen of pancreatic cancer was immunostained with antibodies specific to Fz2. Cell proliferation assays were performed with MIA‑Paca2 cells transfected with small interfering RNA (siRNA) or short hairpin RNA (shRNA) of Fz2. Fz2 was found to be expressed in all pancreatic cancer cell lines, with the exception of NOR‑P1. Immunostaining revealed that Fz2 was not expressed in normal pancreatic tissues, while it was expressed in pancreatic cancer cells. The expression levels of cyclin D1 were analyzed by quantitative PCR. The proliferation and expression of cyclin D1 were suppressed with the siRNA and shRNA of Fz2 in the MIA‑Paca2 cells. Therefore, Fz2 is a potential target for the molecular therapy of pancreatic cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Frizzled‑2: A potential novel target for molecular pancreatic cancer therapy. Oncol Lett 7: 74-78, 2014.
APA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., & Yoshida, T. (2014). Frizzled‑2: A potential novel target for molecular pancreatic cancer therapy. Oncology Letters, 7, 74-78. https://doi.org/10.3892/ol.2013.1681
MLA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Frizzled‑2: A potential novel target for molecular pancreatic cancer therapy". Oncology Letters 7.1 (2014): 74-78.
Chicago
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Frizzled‑2: A potential novel target for molecular pancreatic cancer therapy". Oncology Letters 7, no. 1 (2014): 74-78. https://doi.org/10.3892/ol.2013.1681
Copy and paste a formatted citation
x
Spandidos Publications style
Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Frizzled‑2: A potential novel target for molecular pancreatic cancer therapy. Oncol Lett 7: 74-78, 2014.
APA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., & Yoshida, T. (2014). Frizzled‑2: A potential novel target for molecular pancreatic cancer therapy. Oncology Letters, 7, 74-78. https://doi.org/10.3892/ol.2013.1681
MLA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Frizzled‑2: A potential novel target for molecular pancreatic cancer therapy". Oncology Letters 7.1 (2014): 74-78.
Chicago
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Frizzled‑2: A potential novel target for molecular pancreatic cancer therapy". Oncology Letters 7, no. 1 (2014): 74-78. https://doi.org/10.3892/ol.2013.1681
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