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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Expression of dedifferentiation markers and multilineage markers in U251 glioblastoma cells with silenced EGFR and FGFR genes

  • Authors:
    • Jue Xie
    • Yue‑Hui Ma
    • Ming Wan
    • Ren‑Ya Zhan
    • Yong‑Qing Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Blood Transfusion, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Department of Neurosurgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
  • Pages: 131-136
    |
    Published online on: November 18, 2013
       https://doi.org/10.3892/ol.2013.1685
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Abstract

Epithelial growth factor (EGF) and basic fibroblast growth factor (bFGF), and their receptors, epithelial growth factor receptor (EGFR) and bFGF receptor (bFGFR), are frequently overexpressed in high‑grade gliomas. In the present study, the EGF and bFGF levels in U251 glioblastoma cell culture supernatants were determined by ELISA, and enhanced green fluorescent protein (EGFP)‑labeled recombinant lentiviral expression vectors with small interfering RNA targeting the EGFR and bFGFR genes were constructed. The mRNA expression levels of EGFR, bFGFR, cluster of differentiation (CD)133, glial fibrillary acidic protein (GFAP), tubulin‑β3 (TUBB3) and myelin basic protein (MBP) were determined using quantitative polymerase chain reactions in U251 cells prior to and following silencing of the EGFR and/or bFGFR genes. Prior to silencing, the U251 cells secreted EGF and bFGF, and expressed EGFR, bFGFR, CD133, GFAP, TUBB3 and MBP mRNA. Subsequent to silencing the EGFR and/or bFGFR gene, CD133 mRNA expression decreased and GFAP and TUBB3 mRNA expression increased. Silencing the EGFR and FGFR genes acted synergistically to downregulate CD133 expression. The downregulation of CD133 mRNA expression and the upregulation of GFAP and TUBB3 mRNA expression were not significantly different when blocking the EGFR and FGFR pathways. These results indicate that autocrine or paracrine EGF and/or FGF mechanisms exist in U251 cells. Knocking down the EGFR and/or FGFR genes downregulates CD133 mRNA expression and facilitates glial and neuronal differentiation in U251 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Xie J, Ma YH, Wan M, Zhan RY and Zhou YQ: Expression of dedifferentiation markers and multilineage markers in U251 glioblastoma cells with silenced EGFR and FGFR genes. Oncol Lett 7: 131-136, 2014.
APA
Xie, J., Ma, Y., Wan, M., Zhan, R., & Zhou, Y. (2014). Expression of dedifferentiation markers and multilineage markers in U251 glioblastoma cells with silenced EGFR and FGFR genes. Oncology Letters, 7, 131-136. https://doi.org/10.3892/ol.2013.1685
MLA
Xie, J., Ma, Y., Wan, M., Zhan, R., Zhou, Y."Expression of dedifferentiation markers and multilineage markers in U251 glioblastoma cells with silenced EGFR and FGFR genes". Oncology Letters 7.1 (2014): 131-136.
Chicago
Xie, J., Ma, Y., Wan, M., Zhan, R., Zhou, Y."Expression of dedifferentiation markers and multilineage markers in U251 glioblastoma cells with silenced EGFR and FGFR genes". Oncology Letters 7, no. 1 (2014): 131-136. https://doi.org/10.3892/ol.2013.1685
Copy and paste a formatted citation
x
Spandidos Publications style
Xie J, Ma YH, Wan M, Zhan RY and Zhou YQ: Expression of dedifferentiation markers and multilineage markers in U251 glioblastoma cells with silenced EGFR and FGFR genes. Oncol Lett 7: 131-136, 2014.
APA
Xie, J., Ma, Y., Wan, M., Zhan, R., & Zhou, Y. (2014). Expression of dedifferentiation markers and multilineage markers in U251 glioblastoma cells with silenced EGFR and FGFR genes. Oncology Letters, 7, 131-136. https://doi.org/10.3892/ol.2013.1685
MLA
Xie, J., Ma, Y., Wan, M., Zhan, R., Zhou, Y."Expression of dedifferentiation markers and multilineage markers in U251 glioblastoma cells with silenced EGFR and FGFR genes". Oncology Letters 7.1 (2014): 131-136.
Chicago
Xie, J., Ma, Y., Wan, M., Zhan, R., Zhou, Y."Expression of dedifferentiation markers and multilineage markers in U251 glioblastoma cells with silenced EGFR and FGFR genes". Oncology Letters 7, no. 1 (2014): 131-136. https://doi.org/10.3892/ol.2013.1685
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