Acquired resistance L747S mutation in an epidermal growth factor receptor‑tyrosine kinase inhibitor‑naïve patient: A report of three cases

  • Authors:
    • Fumihiro  Yamaguchi
    • Kunihiko  Fukuchi
    • Yohei  Yamazaki
    • Hiromi Takayasu
    • Sakiko  Tazawa
    • Hidetsugu  Tateno
    • Eisuke  Kato
    • Aya  Wakabayashi
    • Mami  Fujimori
    • Takuya  Iwasaki
    • Makoto  Hayashi
    • Yutaka  Tsuchiya
    • Jun  Yamashita
    • Norikazu  Takeda
    • Fumio  Kokubu
  • View Affiliations

  • Published online on: November 25, 2013     https://doi.org/10.3892/ol.2013.1705
  • Pages: 357-360
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The purpose of the present study was to report cases of epidermal growth factor receptor‑tyrosine kinase inhibitor (EGFR‑TKI)‑naïve patients carrying a mutation associated with acquired resistance to the drug. Gene alterations in 77 lung carcinoma patients were analyzed by collecting and studying curette lavage fluid at the time of diagnosis. PCRs were performed to amplify mutation hotspot regions in EGFR genes. The PCR products were direct‑sequenced and the mutations confirmed by resequencing using different primers. Case 1 was a 78‑year‑old Japanese male diagnosed with stage IB lung adenocarcinoma who was found to have two EGFR mutations, G719S and L747S. Case 2 was a 73‑year‑old Japanese male diagnosed with stage IV squamous cell lung carcinoma and bone metastasis who had the EGFR mutation, L747S. Case 3 was an 82‑year‑old Japanese male diagnosed with hyponatremia due to inappropriate secretion of antidiuretic hormone and stage IIIB small cell lung carcinoma (SCLC) who had the EGFR mutation, L747S. Thus, the EGFR mutation L747S associated with acquired EGFR‑TKI resistance was detected in two non‑small cell lung carcinoma (NSCLC) patients and one SCLC patient, none of whom had ever received EGFR‑TKI. The patients were current smokers with stages at diagnosis ranging from IB to IV, and their initial tumors contained resistant clones carrying L747S. L747S may be associated with primary resistance. To the best of our knowledge, this study is the first report of an EGFR mutation associated with resistance to EGFR‑TKI in SCLC patients. The early detection of EGFR‑TKI resistance mutations may be beneficial in making treatment decisions for lung carcinoma patients, including those with SCLC.
View Figures
View References

Related Articles

Journal Cover

2014-February
Volume 7 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yamaguchi F, Fukuchi K, Yamazaki Y, Takayasu H, Tazawa S, Tateno H, Kato E, Wakabayashi A, Fujimori M, Iwasaki T, Iwasaki T, et al: Acquired resistance L747S mutation in an epidermal growth factor receptor‑tyrosine kinase inhibitor‑naïve patient: A report of three cases. Oncol Lett 7: 357-360, 2014.
APA
Yamaguchi, F., Fukuchi, K., Yamazaki, Y., Takayasu, H., Tazawa, S., Tateno, H. ... Kokubu, F. (2014). Acquired resistance L747S mutation in an epidermal growth factor receptor‑tyrosine kinase inhibitor‑naïve patient: A report of three cases. Oncology Letters, 7, 357-360. https://doi.org/10.3892/ol.2013.1705
MLA
Yamaguchi, F., Fukuchi, K., Yamazaki, Y., Takayasu, H., Tazawa, S., Tateno, H., Kato, E., Wakabayashi, A., Fujimori, M., Iwasaki, T., Hayashi, M., Tsuchiya, Y., Yamashita, J., Takeda, N., Kokubu, F."Acquired resistance L747S mutation in an epidermal growth factor receptor‑tyrosine kinase inhibitor‑naïve patient: A report of three cases". Oncology Letters 7.2 (2014): 357-360.
Chicago
Yamaguchi, F., Fukuchi, K., Yamazaki, Y., Takayasu, H., Tazawa, S., Tateno, H., Kato, E., Wakabayashi, A., Fujimori, M., Iwasaki, T., Hayashi, M., Tsuchiya, Y., Yamashita, J., Takeda, N., Kokubu, F."Acquired resistance L747S mutation in an epidermal growth factor receptor‑tyrosine kinase inhibitor‑naïve patient: A report of three cases". Oncology Letters 7, no. 2 (2014): 357-360. https://doi.org/10.3892/ol.2013.1705