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Article

Construction of a tumor cell‑targeting non‑viral gene delivery vector with polyethylenimine modified with RGD sequence‑containing peptide

  • Authors:
    • Hai‑Bo Xing
    • Hong‑Ming Pan
    • Yong Fang
    • Xiao‑Yun Zhou
    • Qin Pan
    • Da Li
  • View Affiliations / Copyright

    Affiliations: Department of Intensive Care Unit, Xiasha Hospital, Hangzhou, Zhejiang 310019, P.R. China, Department of Medical Oncology, Sir Run Run Shaw Hospital Affiliated to School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, P.R. China
  • Pages: 487-492
    |
    Published online on: November 29, 2013
       https://doi.org/10.3892/ol.2013.1717
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Abstract

The objective of the present study was to construct a novel type of non‑viral gene delivery vector with high delivery efficiency and specific tumor cell‑targeting ability. The CP9 peptide (CYGGRGDTP) containing Arg‑Gly‑Asp sequence was employed to be conjugated onto polyethylenimine (PEI) to act as the role of the targeting moiety. The chemical linker, N‑succinimidyl‑3‑(2‑pyridyldithio) propionate, was applied during the synthesis of the vector (CP9‑PEI). The physicochemical characteristics of the vector were evaluated by the methods of 1H‑nuclear magnetic resonance, Fourier transform infrared spectroscopy, gel retardation assay, electron microscope observation and particle size detection. HepG2 cells were used to verify the gene delivery efficiency and targeting ability by gene delivery procedure and free CP9 peptide inhibition tests. The results showed that the successful synthesis of CP9‑PEI and the synthesized vector may efficiently condense plasmid DNA into round particles with diameters of ~200 nm at a polymer/pDNA ratio of 10. CP9‑PEI may deliver the reporter gene into HepG2 cells with higher efficiency and the efficiency may be inhibited by the free CP9 peptide. The present study suggested that the modification of PEI with the CP9 peptide is an effective method to construct a novel tumor cell‑targeting non‑viral vector, and that the novel vector exhibits great prospect in the field of cancer gene therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Xing HB, Pan HM, Fang Y, Zhou XY, Pan Q and Li D: Construction of a tumor cell‑targeting non‑viral gene delivery vector with polyethylenimine modified with RGD sequence‑containing peptide. Oncol Lett 7: 487-492, 2014.
APA
Xing, H., Pan, H., Fang, Y., Zhou, X., Pan, Q., & Li, D. (2014). Construction of a tumor cell‑targeting non‑viral gene delivery vector with polyethylenimine modified with RGD sequence‑containing peptide. Oncology Letters, 7, 487-492. https://doi.org/10.3892/ol.2013.1717
MLA
Xing, H., Pan, H., Fang, Y., Zhou, X., Pan, Q., Li, D."Construction of a tumor cell‑targeting non‑viral gene delivery vector with polyethylenimine modified with RGD sequence‑containing peptide". Oncology Letters 7.2 (2014): 487-492.
Chicago
Xing, H., Pan, H., Fang, Y., Zhou, X., Pan, Q., Li, D."Construction of a tumor cell‑targeting non‑viral gene delivery vector with polyethylenimine modified with RGD sequence‑containing peptide". Oncology Letters 7, no. 2 (2014): 487-492. https://doi.org/10.3892/ol.2013.1717
Copy and paste a formatted citation
x
Spandidos Publications style
Xing HB, Pan HM, Fang Y, Zhou XY, Pan Q and Li D: Construction of a tumor cell‑targeting non‑viral gene delivery vector with polyethylenimine modified with RGD sequence‑containing peptide. Oncol Lett 7: 487-492, 2014.
APA
Xing, H., Pan, H., Fang, Y., Zhou, X., Pan, Q., & Li, D. (2014). Construction of a tumor cell‑targeting non‑viral gene delivery vector with polyethylenimine modified with RGD sequence‑containing peptide. Oncology Letters, 7, 487-492. https://doi.org/10.3892/ol.2013.1717
MLA
Xing, H., Pan, H., Fang, Y., Zhou, X., Pan, Q., Li, D."Construction of a tumor cell‑targeting non‑viral gene delivery vector with polyethylenimine modified with RGD sequence‑containing peptide". Oncology Letters 7.2 (2014): 487-492.
Chicago
Xing, H., Pan, H., Fang, Y., Zhou, X., Pan, Q., Li, D."Construction of a tumor cell‑targeting non‑viral gene delivery vector with polyethylenimine modified with RGD sequence‑containing peptide". Oncology Letters 7, no. 2 (2014): 487-492. https://doi.org/10.3892/ol.2013.1717
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