Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
2014-March Volume 7 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-March Volume 7 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Detection and pathological value of papillomavirus DNA and p16INK4A and p53 protein expression in cervical intraepithelial neoplasia

  • Authors:
    • Jingbo Wu
    • Xiao‑Jing Li
    • Wei Zhu
    • Xiu‑Ping Liu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China
  • Pages: 738-744
    |
    Published online on: January 14, 2014
       https://doi.org/10.3892/ol.2014.1791
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate human papillomavirus (HPV) infection and p16INK4A and p53 protein expression, to evaluate their roles in the pathological diagnosis and grading for cervical intraepithelial neoplasia (CIN). The detection of HPV DNA and p16INK4A and p53 protein expression were examined in a panel of clinical tissue samples using polymerase chain reaction or immunohistochemistry. In 104 cases, HPV16/18 DNA was identified in 49.0% and HPV6/11 DNA in 9.6% of cases. While in 203 cases, 74.4% positively expressed p16INK4A and 47.3% positively expressed p53. The expression of p16INK4A exhibited a significantly higher rate in the CIN I group than in the squamous metaplasia coupled with hyperplasia group (SMH; P<0.0001) and the CIN II‑III group (P=0.005). A marked correlation was revealed between the band‑like staining pattern of p16INK4A and HPV16/18 infection. On the contrary, p53 expression was not found to significantly correlate with CIN grade or the HPV16/18 infection status. These results suggested that p16INK4A expression correlates with a higher grade of CIN and may be used as a diagnostic marker to distinguish between CIN I and SMH, as well as between CIN I and CIN II‑III.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Bauer HM and Ault K: Human papillomavirus: Current prevalence and future Protection. Sex Trans Dis. 33:509–511. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Chih HJ, Lee AH, Colville L, Binns CW and Xu D: A review of dietary prevention of human papillomavirus-related infection of the cervix and cervicalintraepithelial neoplasia. Nutr Cancer. 65:317–328. 2013. View Article : Google Scholar

3 

Qian JH, Xie X and Yie DF: Epidemiologic features of human paplillomavirus infection in cervix. Chin J Obstet Gynecol. 38:712–714. 2006.

4 

Martin CM and O’Leary JJ: Histology of cervical intraepithelial neoplasia and the role of biomarkers. Best Pract Res Clin Obstet Gynaecol. 25:605–615. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Dascau V, Furau G, Furau C, Paiusan L, et al: Cervical intraepithelial neoplasia in the ‘dr. Salvator vuia’ clinical obstetrics and gynecology hospital - arad during the 2000–2009 period. Maedica (Buchar). 7:138–142. 2012.

6 

Walts AE, Lechago LJ and Bose S: P16 and Ki67 immunostaining is a useful adjunct in the assessment of biopsies for HPV-associated anal intraepithelial neoplasia. Am J Surg Pathol. 30:795–801. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Monsonego J, Pintos J, Semaille C, Beumont M, et al: Human papillomavirus testing improves the accuracy of colposcopy in detection of cervical intraepithelial neoplasia. Int J Gynecol Cancer. 16:591–598. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Massad LS, Einstein MH, Huh WK and Katki HA: 2012 updated consensus guidelines for the management of abnormal cervical cancer screening tests and cancer precursors. J Low Genit Tract Dis. 17(5 Suppl 1): S1–S27. 2013. View Article : Google Scholar

9 

Tsoumpou I, Arbyn M, Kyrgiou M, Wentzensen N, et al: p16(INK4a) immunostaining in cytological and histological specimens from the uterine cervix: a systematic review and meta-analysis. Cancer Treat Rev. 35:210–220. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Sano T, Oyama T, Kashiwabara K, Fukuda T and Nakajima T: Expression status of p16 protein is associated with human papillomavirus oncogenic potential in cervical and genital lesions. Am J Pathol. 153:1741–1748. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Sotlar K, Diemer D, Dethleffs A, Hack Y, et al: Detection and typing of human papillomavirus by E6 nested multiplex PCR. J Clin Microbiol. 42:3176–3184. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Gheit T and Tommasino M: Detection of high-risk mucosal human papillomavirus DNA in human specimens by a novel and sensitive multiplex PCR method combined with DNA microarray. Methods Mol Biol. 665:195–212. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Shirendeb U, Hishikawa Y, Moriyama S, Win N, et al: Human papillomavirus infection and its possible correlation with p63 expression in cervical cancer in Japan, Mongolia, and Myanmar. Acta Histochem Cytochem. 42:181–190. 2009. View Article : Google Scholar

14 

Lax S: Histopathology of cervical precursor lesions and cancer. Acta Dermatovenerol Alp Panonica Adriat. 20:125–133. 2011.PubMed/NCBI

15 

Sakaguchi M, Fujii Y, Hirabayashi H, Yoon HE, et al: Inversely correlated expression of p16 and Rb protein in non-small cell lung cancers: an immunohistochemical study. Int J Cancer. 65:442–445. 1996. View Article : Google Scholar : PubMed/NCBI

16 

Sano T, Oyama T, Kashiwabara K, Fukuda T and Nakajima T: Immunohistochemical overexpression of p16 protein associated with intact retinoblastoma protein expression in cervical cancer and cervical intraepithelial neoplasia. Pathol Int. 48:580–585. 1998. View Article : Google Scholar

17 

Koshiol J, Lindsay L, Pimenta JM, Poole C, et al: Persistent human papillomavirus infection and cervical neoplasia: a systematic review and meta-analysis. Am J Epidemiol. 168:123–137. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Carozzi F, Gillio-Tos A, Confortini M, et al: Risk of high-grade cervical intraepithelial neoplasia during follow-up in HPV-positive women according to baseline p16-INK4A results: a prospective analysis of a nested substudy of the NTCC randomised controlled trial. Lancet Oncol. 14:168–176. 2013. View Article : Google Scholar

19 

Lesnikova I, Lidang M, Hamilton-Dutoit S and Koch J: p16 as a diagnostic marker of cervical neoplasia: a tissue microarray study of 796 archival specimens. Diagn Pathol. 4:222009. View Article : Google Scholar

20 

Lee S, Kim H, Kim H, Kim C and Kim I: The utility of p16INK4a and Ki-67 as a conjunctive tool in uterine cervical lesions. Korean J Pathol. 46:253–260. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Lin ZH, Liu MZ, Zhao YW, Wu QY, et al: Study of p16 expression and DNA ploidy in HPV-negative cervical cancers and precursors. Chin J Pathol. 35:412–416. 2006.(In Chinese).

22 

Soma M and Kamaraj S: Detection of human papillomavirus in cervical gradings by immunohistochemistry and typing of HPV 16 and 18 in high-grades by polymerase chain reaction. J Lab Physicians. 2:31–36. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Samir R, Asplund A, Tot T, Pekar G and Hellberg D: High-risk HPV infection and CIN grade correlates to the expression of c-myc, CD4+, FHIT, E-cadherin, Ki-67, and p16INK4a. J Low Genit Tract Dis. 15:280–286. 2011.PubMed/NCBI

24 

Yim EK and Park JS: Biomarkers in cervical cancer. Biomark Insights. 7:215–225. 2007.

25 

Kuo KT, Chang HC, Hsiao CH and Lin MC: Increased Ki-67 proliferative index and absence of P16INK4 in CIN-HPV related pathogenic pathways different from cervical squamous intraepithelial lesion. Br J Ophthalmol. 90:894–899. 2006. View Article : Google Scholar

26 

Kong CS, Balzer BL, Troxell ML, Patterson BK and Longacre TA: p16INK4A immunohistochemistry is superior to HPV in situ hybridization for the detection of high-risk HPV in atypical squamous metaplasia. Am J Surg Pathol. 31:33–43. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Bragança JF, Sarian LO, Pitta DR, Maito AB, et al: Expression of p16(INK4a) and cervical infection with high-risk human papillomavirus are not related to p53 activity in cervical intraepithelial neoplasia. Int J Gynecol Cancer. 18:1060–1064. 2008.PubMed/NCBI

28 

Türkçüoğlu I, Tezcan S, Kaygusuz G, Atabekoğlu CS, et al: The role of p53, Bcl-2 and Ki-67 in premalignant cervical lesions and cervical cancer. Eur J Gynaecol Oncol. 28:290–293. 2007.PubMed/NCBI

29 

Giannoudis and Herrington CS: Differential expression of p53 and p21 in low grade cervical squamous intraepithelial lesions infected with low, intermediate, and high risk human papillomaviruses. Cancer. 89:1300–1307. 2000. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wu J, Li XJ, Zhu W and Liu XP: Detection and pathological value of papillomavirus DNA and p16INK4A and p53 protein expression in cervical intraepithelial neoplasia. Oncol Lett 7: 738-744, 2014.
APA
Wu, J., Li, X., Zhu, W., & Liu, X. (2014). Detection and pathological value of papillomavirus DNA and p16INK4A and p53 protein expression in cervical intraepithelial neoplasia. Oncology Letters, 7, 738-744. https://doi.org/10.3892/ol.2014.1791
MLA
Wu, J., Li, X., Zhu, W., Liu, X."Detection and pathological value of papillomavirus DNA and p16INK4A and p53 protein expression in cervical intraepithelial neoplasia". Oncology Letters 7.3 (2014): 738-744.
Chicago
Wu, J., Li, X., Zhu, W., Liu, X."Detection and pathological value of papillomavirus DNA and p16INK4A and p53 protein expression in cervical intraepithelial neoplasia". Oncology Letters 7, no. 3 (2014): 738-744. https://doi.org/10.3892/ol.2014.1791
Copy and paste a formatted citation
x
Spandidos Publications style
Wu J, Li XJ, Zhu W and Liu XP: Detection and pathological value of papillomavirus DNA and p16INK4A and p53 protein expression in cervical intraepithelial neoplasia. Oncol Lett 7: 738-744, 2014.
APA
Wu, J., Li, X., Zhu, W., & Liu, X. (2014). Detection and pathological value of papillomavirus DNA and p16INK4A and p53 protein expression in cervical intraepithelial neoplasia. Oncology Letters, 7, 738-744. https://doi.org/10.3892/ol.2014.1791
MLA
Wu, J., Li, X., Zhu, W., Liu, X."Detection and pathological value of papillomavirus DNA and p16INK4A and p53 protein expression in cervical intraepithelial neoplasia". Oncology Letters 7.3 (2014): 738-744.
Chicago
Wu, J., Li, X., Zhu, W., Liu, X."Detection and pathological value of papillomavirus DNA and p16INK4A and p53 protein expression in cervical intraepithelial neoplasia". Oncology Letters 7, no. 3 (2014): 738-744. https://doi.org/10.3892/ol.2014.1791
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team