Robust linear regression model of Ki‑67 for mitotic rate in gastrointestinal stromal tumors

Kemmerling,Ralf; Weyland,Denis; Kiesslich,Tobias; Illig,Romana; Klieser,Eckhard; Jäger,Tarkan; Dietze,Otto; Neureiter,Daniel

doi:10.3892/ol.2014.1802

2014-March Volume 7 Issue 3

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2014-March Volume 7 Issue 3

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Article

Robust linear regression model of Ki‑67 for mitotic rate in gastrointestinal stromal tumors

Authors:
- Ralf Kemmerling
- Denis Weyland
- Tobias Kiesslich
- Romana Illig
- Eckhard Klieser
- Tarkan Jäger
- Otto Dietze
- Daniel Neureiter
View Affiliations / Copyright

Affiliations: Institute of Pathology, Paracelsus Medical University/Salzburger Landeskliniken, Salzburg A‑5020, Austria, Department of Internal Medicine I, Paracelsus Medical University/Salzburger Landeskliniken, Salzburg A‑5020, Austria, Department of Surgery, Paracelsus Medical University/Salzburger Landeskliniken, Salzburg A‑5020, Austria
Pages: 745-749
|
Published online on: January 15, 2014

https://doi.org/10.3892/ol.2014.1802
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Abstract

Risk stratification of gastrointestinal stromal tumors (GISTs) by tumor size, lymph node and metastasis status is crucially affected by mitotic activity. To date, no studies have quantitatively compared mitotic activity in hematoxylin and eosin (H&E)‑stained tissue sections with immunohistochemical markers, such as phosphohistone H3 (PHH3) and Ki‑67. According to the TNM guidelines, the mitotic count on H&E sections and immunohistochemical PHH3‑stained slides has been assessed per 50 high‑power fields of 154 specimens of clinically documented GIST cases. The Ki‑67‑associated proliferation rate was evaluated on three digitalized hot spots using image analysis. The H&E‑based mitotic rate was found to correlate significantly better with Ki‑67‑assessed proliferation activity than with PHH3‑assessed proliferation activity (r=0.780; P<0.01). A linear regression model (analysis of variance; P<0.001) allowed reliable predictions of the H&E‑associated mitoses based on the Ki‑67 expression alone. Additionally, the Ki‑67‑associated proliferation revealed a higher and significant impact on the recurrence and metastasis rate of the GIST cases than by the classical H&E‑based mitotic rate. The results of the present study indicated that the mitotic rate may be reliably and time‑efficiently estimated by immunohistochemistry of Ki‑67 using only three hot spots.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Robust linear regression model of Ki‑67 for mitotic rate in gastrointestinal stromal tumors

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Abstract

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