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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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2014-March Volume 7 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells

  • Authors:
    • Chengliang Sun
    • Chuanju Liu
    • Jun Dong
    • Dong Li
    • Wei Li
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Shandong University-Affiliated Provincial Hospital, Jinan, Shandong 250021, P.R. China, Department of Orthopaedic Surgery and Cell Biology, New York University School of Medicine, New York, NY 10003, USA
  • Pages: 815-819
    |
    Published online on: January 17, 2014
       https://doi.org/10.3892/ol.2014.1811
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Abstract

Despite improvements over the past two decades, the outcome for patients with advanced osteosarcoma remains poor. Targeted therapies have emerged as promising treatment options for various malignancies. However, effective targeted cancer therapies require the identification of key molecules in the pathogenesis of cancer. The aim of this study was to evaluate the value of the myeloid cell nuclear differentiation antigen (MNDA), a member of the interferon-inducible p200 (IFI-200) family, as a therapeutic target for osteosarcoma by analyzing the baseline expression of MNDA in human osteosarcoma cells and determining the effect of MNDA overexpression on the proliferation and apoptosis profiles and migration/invasion ability in osteosarcoma cells. To this end, MNDA mRNA abundance in wild-type sarcoma osteogenic (Saos-2) cells was analyzed using reverse transcription-polymerase chain reaction, proliferation/apoptosis profiles and migration/invasion capacity in Saos-2 cells overexpressing a green fluorescence protein (GFP)-human MNDA fusion protein. Saos-2 cells found to be overexpressing GFP alone were assessed by 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis and Matrigel Transwell migration assay. The results demonstrated that MNDA mRNA was significantly less abundant in wild-type Saos-2 cells compared with human monocyte-like U-937 cells and MNDA overexpression effectively inhibited proliferation, induced apoptosis and reduced migration/invasiveness in Saos-2 cells compared with GFP overexpression alone. Preliminary observations suggested that MNDA potentially serves as a novel therapeutic target for osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Sun C, Liu C, Dong J, Li D and Li W: Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells. Oncol Lett 7: 815-819, 2014.
APA
Sun, C., Liu, C., Dong, J., Li, D., & Li, W. (2014). Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells. Oncology Letters, 7, 815-819. https://doi.org/10.3892/ol.2014.1811
MLA
Sun, C., Liu, C., Dong, J., Li, D., Li, W."Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells". Oncology Letters 7.3 (2014): 815-819.
Chicago
Sun, C., Liu, C., Dong, J., Li, D., Li, W."Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells". Oncology Letters 7, no. 3 (2014): 815-819. https://doi.org/10.3892/ol.2014.1811
Copy and paste a formatted citation
x
Spandidos Publications style
Sun C, Liu C, Dong J, Li D and Li W: Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells. Oncol Lett 7: 815-819, 2014.
APA
Sun, C., Liu, C., Dong, J., Li, D., & Li, W. (2014). Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells. Oncology Letters, 7, 815-819. https://doi.org/10.3892/ol.2014.1811
MLA
Sun, C., Liu, C., Dong, J., Li, D., Li, W."Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells". Oncology Letters 7.3 (2014): 815-819.
Chicago
Sun, C., Liu, C., Dong, J., Li, D., Li, W."Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells". Oncology Letters 7, no. 3 (2014): 815-819. https://doi.org/10.3892/ol.2014.1811
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