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Article

Inhibition of cell growth by BrMC through inactivation of Akt in HER‑2/neu‑overexpressing breast cancer cells

  • Authors:
    • Xiao‑Zheng Cao
    • Hong‑Lin Xiang
    • Mei‑Fang Quan
    • Li‑Hua He
  • View Affiliations / Copyright

    Affiliations: Medical College, Hunan Normal University, Changsha, Hunan 410013, P.R. China
  • Pages: 1632-1638
    |
    Published online on: February 18, 2014
       https://doi.org/10.3892/ol.2014.1889
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Abstract

We previously reported that chrysin (ChR) and its analogs induced cell cycle arrest and apoptosis in human estrogen receptor‑positive/‑negative breast cancer cells. However, it was unknown whether 8‑bromo‑7‑methoxychrysin (BrMC), a novel synthetic ChR analog, inhibited the cell growth of human epidermal growth factor receptor 2 (HER‑2)/neu‑overexpressing breast cancers. In the present study, it was demonstrated that BrMC preferentially inhibited the cell viability of HER‑2/neu‑overexpressing MDA‑MB‑453 and BT‑474 cells. Western blot analysis revealed that HER‑2/neu expression and tyrosine phosphorylation were inhibited by BrMC in a concentration‑dependent manner; whereas the proteasome inhibitor, MG‑132, significantly prevented BrMC‑induced HER‑2/neu depletion and cell death in MDA‑MB‑453 cells. This directly indicated that BrMC‑induced HER‑2/neu depletion and cell growth inhibition was mediated by a proteasomal pathway. BrMC significantly downregulated the expression of cyclin D1, cyclin E and CDK4, followed by the suppression of protein kinase B phosphorylation and downstream effectors, GSK‑3β and β‑catenin. A colony formation assay also confirmed the growth‑inhibitory effects of BrMC. Thus, these findings clearly demonstrate the anticancer activity of BrMC against human HER‑2/neu‑overexpressing breast cancer cells. Thus, these findings clearly demonstrate the anticancer activity of BrMC against human HER 2/neu-overexpressing breast cancer cells, and highlight BrMC as a promising candidate for breast cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Cao XZ, Xiang HL, Quan MF and He LH: Inhibition of cell growth by BrMC through inactivation of Akt in HER‑2/neu‑overexpressing breast cancer cells. Oncol Lett 7: 1632-1638, 2014.
APA
Cao, X., Xiang, H., Quan, M., & He, L. (2014). Inhibition of cell growth by BrMC through inactivation of Akt in HER‑2/neu‑overexpressing breast cancer cells. Oncology Letters, 7, 1632-1638. https://doi.org/10.3892/ol.2014.1889
MLA
Cao, X., Xiang, H., Quan, M., He, L."Inhibition of cell growth by BrMC through inactivation of Akt in HER‑2/neu‑overexpressing breast cancer cells". Oncology Letters 7.5 (2014): 1632-1638.
Chicago
Cao, X., Xiang, H., Quan, M., He, L."Inhibition of cell growth by BrMC through inactivation of Akt in HER‑2/neu‑overexpressing breast cancer cells". Oncology Letters 7, no. 5 (2014): 1632-1638. https://doi.org/10.3892/ol.2014.1889
Copy and paste a formatted citation
x
Spandidos Publications style
Cao XZ, Xiang HL, Quan MF and He LH: Inhibition of cell growth by BrMC through inactivation of Akt in HER‑2/neu‑overexpressing breast cancer cells. Oncol Lett 7: 1632-1638, 2014.
APA
Cao, X., Xiang, H., Quan, M., & He, L. (2014). Inhibition of cell growth by BrMC through inactivation of Akt in HER‑2/neu‑overexpressing breast cancer cells. Oncology Letters, 7, 1632-1638. https://doi.org/10.3892/ol.2014.1889
MLA
Cao, X., Xiang, H., Quan, M., He, L."Inhibition of cell growth by BrMC through inactivation of Akt in HER‑2/neu‑overexpressing breast cancer cells". Oncology Letters 7.5 (2014): 1632-1638.
Chicago
Cao, X., Xiang, H., Quan, M., He, L."Inhibition of cell growth by BrMC through inactivation of Akt in HER‑2/neu‑overexpressing breast cancer cells". Oncology Letters 7, no. 5 (2014): 1632-1638. https://doi.org/10.3892/ol.2014.1889
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