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Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells

  • Authors:
    • Shuqun Zhang
    • Yinan Ma
    • Jiantao Jiang
    • Zhijun Dai
    • Xiaoyan Gao
    • Xiaoran Yin
    • Wentao Xi
    • Weili Min
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 1375-1380
    |
    Published online on: February 27, 2014
       https://doi.org/10.3892/ol.2014.1918
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Abstract

The aim of the present study was to investigate the inhibitory effects of dihydroartemisinin (DHA) on the primary tumor growth and metastasis of the human breast cancer cell line, MDA‑MB‑231, in vitro. The expression levels of urokinase‑type plasminogen activator (uPA) were detected by immunocytochemistry in two cell lines (MCF‑7 and MDA‑MB‑231). The MDA‑MB‑231 cell activity was inhibited by various concentration gradients of DHA. The inhibitory rate, cell growth curve and apoptotic morphological observations were obtained using the MTT assay at 0, 24, 48 and 72 h. Cell scratch migration was performed at various time‑points to test the cell proliferation and migration capacity. Reverse transcription‑polymerase chain reaction was used to analyze the effect of DHA on uPA mRNA expression in breast cancer cells. The human breast cancer cell line, MDA‑MB‑231, possesses higher metastatic potential and relatively higher expression of uPA when compared with the MCF-7 cell line. DHA was found to inhibit the proliferation and migration capacity of the cell line, MDA‑MB‑231, in vitro. The growth inhibition occurred in a time‑ and dose‑dependent manner, with IC50 values of 117.76±0.04, 60.26±0.12 and 52.96±0.07 µmol/l following 24, 48 and 72 h, respectively. The inhibition of uPA was observed to decrease breast cancer cell growth and migration. Thus, results of the present study indicate that DHA may be used for further studies with regard to breast cancer therapy.
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1 

Giannopoulou I, Mylona E, Kapranou A, et al: The prognostic value of the topographic distribution of uPAR expression in invasive breast carcinomas. Cancer Lett. 246:262–267. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Borstnar S, Sadikov A, Mozina B and Cufer T: High levels of uPA and PAI-1 predict a good response to anthracyclines. Breast Cancer Res Treat. 121:615–624. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Jänicke F, Prechtl A, Thomssen C, et al; German N0 Study Group. Randomized adjuvant chemotherapy trial in high-risk, lymph node-negative breast cancer patients identified by urokinase-type plasminogen activator and plasminogen activator inhibitor type 1. J Natl Cancer Inst. 93:913–920. 2001.

4 

Mani T, Wang F, Knabe WE, et al: Small-molecule inhibition of the uPAR·uPA interaction: synthesis, biochemical, cellular, in vivo pharmacokinetics and efficacy studies in breast cancer metastasis. Bioorg Med Chem. 21:2145–2155. 2013.

5 

Harbeck N, Kates RE, Gauger K, et al: Urokinase-type plasminogen activator (uPA) and its inhibitor PAI-I: novel tumor-derived factors with a high prognostic and predictive impact in breast cancer. Thromb Haemost. 91:450–456. 2004.PubMed/NCBI

6 

Efferth T, Dunstan H, Sauerbrey A, Miyachi H and Chitambar CR: The anti-malarial artesunate is also active against cancer. Int J Onco1. 18:767–773. 2001.

7 

Gomme PT, McCann KB and Bertolini J: Transferrin: structure, function and potential therapeutic actions. Drug Discov Today. 10:267–273. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Lu YY, Chen TS, Qu JL, Pan WL, Sun L and Wei XB: Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells. J Biomed Sci. 16:162009. View Article : Google Scholar : PubMed/NCBI

9 

Nakase I, Gallis B, Takatani-Nakase T, et al: Transferrin receptor-dependent cytotoxicity of artemisinin-transferrin conjugates on prostate cancer cells and induction of apoptosis. Cancer Lett. 274:290–298. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Nakase I, Lai H, Singh NP and Sasaki T: Anticancer properties of artemisinin derivatives and their targeted delivery by transferrin conjugation. Int J Pharm. 354:28–33. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Oh S, Kim BJ, Singh NP, Lai H and Sasaki T: Synthesis and anti-cancer activity of covalent conjugates of artemisinin and a transferrin-receptor targeting peptide. Cancer Lett. 274:33–39. 2009. View Article : Google Scholar

12 

Youns M, Efferth T, Reichling J, Fellenberg K, Bauer A and Hoheisel JD: Gene expression profiling identifies novel key players involved in the cytotoxic effect of Artesunate on pancreatic cancer cells. Biochem Pharmacol. 78:273–283. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Wang Z, Yu Y, Ma J, et al: LyP-1 modification to enhance delivery of artemisinin or fluorescent probe loaded polymeric micelles to highly metastatic tumor and its lymphatics. Mol Pharm. 9:2646–2657. 2012. View Article : Google Scholar

14 

Singh NP and Lai H: Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sci. 70:49–56. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Lai H and Singh NP: Oral artemisinin prevents and delays the development of 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer in the rat. Cancer Lett. 231:43–48. 2006.PubMed/NCBI

16 

Li PC, Lam E, Roos WP, Zdzienicka MZ, Kaina B and Efferth T: Artesunate derived from traditional Chinese medicine induces DNA damage and repair. Cancer Res. 68:4347–4351. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Hwang YP, Yun HJ, Kim HG, Han EH, Lee GW and Jeong HG: Suppression of PMA-induced tumor cell invasion by dihydroartemisinin via inhibition of PKCalpha/Raf/MAPKs and NF-kappaB/AP-1-dependent mechanisms. Biochem Pharmacol. 79:1714–1726. 2010. View Article : Google Scholar

18 

De Cremoux P, Grandin L, Diéras V, et al; Breast Cancer Study Group of the Institut Curie. Urokinase-type plasminogen activator and plasminogen-activator-inhibitor type 1 predict metastases in good prognosis breast cancer patients. Anticancer Res. 29:1475–1482. 2009.PubMed/NCBI

19 

Chen H, Sun B, Wang S, Pan S, Gao Y, Bai X and Xue D: Growth inhibitory effects of dihydroartemisinin on pancreatic cancer cells: involvement of cell cycle arrest and inactivation of nuclear factor-kappaB. J Cancer Res Clin Oncol. 136:897–903. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Tan XJ, Plouet J, Lang JH, Wu M and Shen K: Effects of dihydroartiminisin on proliferation and phosphorylation of mitogen-activated protein kinase in epithelial ovarian cancer cell lines. Zhonghua Fu Chan Ke Za Zhi. 43:662–665. 2008.(In Chinese).

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Copy and paste a formatted citation
Spandidos Publications style
Zhang S, Ma Y, Jiang J, Dai Z, Gao X, Yin X, Xi W and Min W: Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells. Oncol Lett 7: 1375-1380, 2014.
APA
Zhang, S., Ma, Y., Jiang, J., Dai, Z., Gao, X., Yin, X. ... Min, W. (2014). Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells. Oncology Letters, 7, 1375-1380. https://doi.org/10.3892/ol.2014.1918
MLA
Zhang, S., Ma, Y., Jiang, J., Dai, Z., Gao, X., Yin, X., Xi, W., Min, W."Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells". Oncology Letters 7.5 (2014): 1375-1380.
Chicago
Zhang, S., Ma, Y., Jiang, J., Dai, Z., Gao, X., Yin, X., Xi, W., Min, W."Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells". Oncology Letters 7, no. 5 (2014): 1375-1380. https://doi.org/10.3892/ol.2014.1918
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Ma Y, Jiang J, Dai Z, Gao X, Yin X, Xi W and Min W: Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells. Oncol Lett 7: 1375-1380, 2014.
APA
Zhang, S., Ma, Y., Jiang, J., Dai, Z., Gao, X., Yin, X. ... Min, W. (2014). Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells. Oncology Letters, 7, 1375-1380. https://doi.org/10.3892/ol.2014.1918
MLA
Zhang, S., Ma, Y., Jiang, J., Dai, Z., Gao, X., Yin, X., Xi, W., Min, W."Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells". Oncology Letters 7.5 (2014): 1375-1380.
Chicago
Zhang, S., Ma, Y., Jiang, J., Dai, Z., Gao, X., Yin, X., Xi, W., Min, W."Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells". Oncology Letters 7, no. 5 (2014): 1375-1380. https://doi.org/10.3892/ol.2014.1918
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