Open Access

Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells

  • Authors:
    • Shuqun Zhang
    • Yinan Ma
    • Jiantao Jiang
    • Zhijun Dai
    • Xiaoyan Gao
    • Xiaoran Yin
    • Wentao Xi
    • Weili Min
  • View Affiliations

  • Published online on: February 27, 2014     https://doi.org/10.3892/ol.2014.1918
  • Pages: 1375-1380
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Abstract

The aim of the present study was to investigate the inhibitory effects of dihydroartemisinin (DHA) on the primary tumor growth and metastasis of the human breast cancer cell line, MDA‑MB‑231, in vitro. The expression levels of urokinase‑type plasminogen activator (uPA) were detected by immunocytochemistry in two cell lines (MCF‑7 and MDA‑MB‑231). The MDA‑MB‑231 cell activity was inhibited by various concentration gradients of DHA. The inhibitory rate, cell growth curve and apoptotic morphological observations were obtained using the MTT assay at 0, 24, 48 and 72 h. Cell scratch migration was performed at various time‑points to test the cell proliferation and migration capacity. Reverse transcription‑polymerase chain reaction was used to analyze the effect of DHA on uPA mRNA expression in breast cancer cells. The human breast cancer cell line, MDA‑MB‑231, possesses higher metastatic potential and relatively higher expression of uPA when compared with the MCF-7 cell line. DHA was found to inhibit the proliferation and migration capacity of the cell line, MDA‑MB‑231, in vitro. The growth inhibition occurred in a time‑ and dose‑dependent manner, with IC50 values of 117.76±0.04, 60.26±0.12 and 52.96±0.07 µmol/l following 24, 48 and 72 h, respectively. The inhibition of uPA was observed to decrease breast cancer cell growth and migration. Thus, results of the present study indicate that DHA may be used for further studies with regard to breast cancer therapy.
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May-2014
Volume 7 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang S, Ma Y, Jiang J, Dai Z, Gao X, Yin X, Xi W and Min W: Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells. Oncol Lett 7: 1375-1380, 2014
APA
Zhang, S., Ma, Y., Jiang, J., Dai, Z., Gao, X., Yin, X. ... Min, W. (2014). Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells. Oncology Letters, 7, 1375-1380. https://doi.org/10.3892/ol.2014.1918
MLA
Zhang, S., Ma, Y., Jiang, J., Dai, Z., Gao, X., Yin, X., Xi, W., Min, W."Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells". Oncology Letters 7.5 (2014): 1375-1380.
Chicago
Zhang, S., Ma, Y., Jiang, J., Dai, Z., Gao, X., Yin, X., Xi, W., Min, W."Inhibition of urokinase‑type plasminogen activator expression by dihydroartemisinin in breast cancer cells". Oncology Letters 7, no. 5 (2014): 1375-1380. https://doi.org/10.3892/ol.2014.1918