Tumor suppressor microRNA‑34a inhibits cell proliferation by targeting Notch1 in renal cell carcinoma

Zhang,Changcun; Mo,Ren; Yin,Binde; Zhou,Libin; Liu,Yongchao; Fan,Jie

doi:10.3892/ol.2014.1931

Tumor suppressor microRNA‑34a inhibits cell proliferation by targeting Notch1 in renal cell carcinoma

Authors:
- Changcun Zhang
- Ren Mo
- Binde Yin
- Libin Zhou
- Yongchao Liu
- Jie Fan
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Affiliations: Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China
Published online on: March 4, 2014 https://doi.org/10.3892/ol.2014.1931
Pages: 1689-1694

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Abstract

MicroRNA‑34a (miR‑34a) is a tumor suppressive microRNA, which induces G1 arrest, apoptosis and senescence by repressing the expression of multiple oncogenes. This study aimed to investigate the biological function and molecular mechanisms of miR‑34a in human renal cell carcinoma (RCC) cells. Quantitative polymerase chain reaction (qPCR) revealed that miR‑34a expression was significantly downregulated in eight of the 10 (80%) RCC tissues compared with adjacent normal tissues. In RCC cell lines, several other target genes of miR‑34a were dysregulated at the mRNA level when the expression of miR‑34a was elevated. In addition, western blot analysis and qPCR revealed that forced expression of miR‑34a downregulated the expression of Notch1 at the protein and mRNA level. The Cell Counting Kit‑8 identified that transient forced expression of miR‑34a inhibited cell growth and resulted in cell cycle arrest, which was evaluated by flow cytometry. Our data demonstrated that miR‑34a inhibits cell proliferation by downregulating Notch1 in RCC cell lines.

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