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Article

Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines

  • Authors:
    • Yuichi Ohnishi
    • Masahiro Watanabe
    • Hiroki Yasui
    • Kenji Kakudo
  • View Affiliations / Copyright

    Affiliations: Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, Chuo‑ku, Osaka 540‑0008, Japan
  • Pages: 1439-1442
    |
    Published online on: March 7, 2014
       https://doi.org/10.3892/ol.2014.1946
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Abstract

Epidermal growth factor (EGF) is present at high concentrations in human saliva and modulates the growth and differentiation of various cancer cells. To elucidate the molecular mechanisms by which EGF affects oral cancer proliferation and invasion, the current study analyzed the Matrigel invasion activity of cultured oral cancer cell lines. Cell proliferation under the influence of EGF was subjected to Matrigel invasion assays, and cell proliferation in the absence of EGF was used as control. Northern blot analyses quantified the invasiveness and tumorigenicity. Chloramphenicol acetyltransferase assay determined the EGF stimulation of matrix metalloproteinase (MMP) 1 expression. EGF increased the number of cells penetrating the Matrigel membrane. Northern blot analysis revealed that MMP1 and cytokeratin 19 expression correlate with EGF. In addition, the morphology of HSC‑3 and SAS cells changed following the addition of EGF to the culture medium. A transient transfection assay revealed that EGF increases the promoter activities of MMP1 in HSC‑3 cells. These observations suggested that EGF increases the invasive activity of oral cancer cells, partly by increasing MMP1, and morphological changes may be induced by altering the composition of cytoskeletal proteins.
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Copy and paste a formatted citation
Spandidos Publications style
Ohnishi Y, Watanabe M, Yasui H and Kakudo K: Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines. Oncol Lett 7: 1439-1442, 2014.
APA
Ohnishi, Y., Watanabe, M., Yasui, H., & Kakudo, K. (2014). Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines. Oncology Letters, 7, 1439-1442. https://doi.org/10.3892/ol.2014.1946
MLA
Ohnishi, Y., Watanabe, M., Yasui, H., Kakudo, K."Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines". Oncology Letters 7.5 (2014): 1439-1442.
Chicago
Ohnishi, Y., Watanabe, M., Yasui, H., Kakudo, K."Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines". Oncology Letters 7, no. 5 (2014): 1439-1442. https://doi.org/10.3892/ol.2014.1946
Copy and paste a formatted citation
x
Spandidos Publications style
Ohnishi Y, Watanabe M, Yasui H and Kakudo K: Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines. Oncol Lett 7: 1439-1442, 2014.
APA
Ohnishi, Y., Watanabe, M., Yasui, H., & Kakudo, K. (2014). Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines. Oncology Letters, 7, 1439-1442. https://doi.org/10.3892/ol.2014.1946
MLA
Ohnishi, Y., Watanabe, M., Yasui, H., Kakudo, K."Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines". Oncology Letters 7.5 (2014): 1439-1442.
Chicago
Ohnishi, Y., Watanabe, M., Yasui, H., Kakudo, K."Effects of epidermal growth factor on the invasive activity and cytoskeleton of oral squamous cell carcinoma cell lines". Oncology Letters 7, no. 5 (2014): 1439-1442. https://doi.org/10.3892/ol.2014.1946
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