Quantitative analysis of APC promoter methylation in hepatocellular carcinoma and its prognostic implications

  • Authors:
    • Baiying Xu
    • Yanfang Nie
    • Xiaoxia Liu
    • Shuqin Feng
    • Zhili  Yang
    • Zhigang Wang
    • Qi Zheng
    • Xiaoying Luo
  • View Affiliations

  • Published online on: March 7, 2014     https://doi.org/10.3892/ol.2014.1951
  • Pages: 1683-1688
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Abstract

The present study aimed to quantitatively determine the aberrant methylation signal of the adenomatous polyposis coli (APC) gene in hepatocellular carcinoma (HCC), and to evaluate whether hypermethylation of the APC promoter could be a prognostic biomarker for HCC. Taqman probe‑based quantitative methylation‑specific polymerase chain reaction was performed to identify the APC promoter methylation levels in 57 HCC and corresponding non‑tumorous liver tissues. In the present study, the methylation level of the APC promoter was upregulated by 4.51‑fold in the HCC tissues compared with the non‑cancerous tissues (P=0.0003). With regard to the clinicopathological data, the methylation level of the APC promoter in the HCC samples was higher in the patients with larger tumors when the cut‑off was set at 4 cm (P=0.0008), and in the older patients when the cut‑off was set at 60 years old (P=0.0438). However, the methylation status in the HCC samples appeared not to affect the overall patient survival rate (P=0.1684). The findings of the present study showed that APC promoter hypermethylation accumulates during the development of HCC, but that it may not be a promising prognostic biomarker for HCC.
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May-2014
Volume 7 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Xu B, Nie Y, Liu X, Feng S, Yang Z, Wang Z, Zheng Q and Luo X: Quantitative analysis of APC promoter methylation in hepatocellular carcinoma and its prognostic implications. Oncol Lett 7: 1683-1688, 2014
APA
Xu, B., Nie, Y., Liu, X., Feng, S., Yang, Z., Wang, Z. ... Luo, X. (2014). Quantitative analysis of APC promoter methylation in hepatocellular carcinoma and its prognostic implications. Oncology Letters, 7, 1683-1688. https://doi.org/10.3892/ol.2014.1951
MLA
Xu, B., Nie, Y., Liu, X., Feng, S., Yang, Z., Wang, Z., Zheng, Q., Luo, X."Quantitative analysis of APC promoter methylation in hepatocellular carcinoma and its prognostic implications". Oncology Letters 7.5 (2014): 1683-1688.
Chicago
Xu, B., Nie, Y., Liu, X., Feng, S., Yang, Z., Wang, Z., Zheng, Q., Luo, X."Quantitative analysis of APC promoter methylation in hepatocellular carcinoma and its prognostic implications". Oncology Letters 7, no. 5 (2014): 1683-1688. https://doi.org/10.3892/ol.2014.1951