Expression of TSG101 protein and LSF transcription factor in HPV‑positive cervical cancer cells

  • Authors:
    • Justyna K. Broniarczyk
    • Alicja Warowicka
    • Anna Kwaśniewska
    • Maria Wolun‑Cholewa
    • Wojciech Kwaśniewski
    • Anna Goździcka‑Józefiak
  • View Affiliations

  • Published online on: March 11, 2014     https://doi.org/10.3892/ol.2014.1967
  • Pages: 1409-1413
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Abstract

Our previous study demonstrated a decreased expression of tumor susceptibility gene 101 (TSG101) in cervical cancer cells. To identify the mechanism responsible for TSG101 downregulation during cervical cancer development, we analyzed the TSG101 promoter using cis‑element cluster finder software. One of the transcription factors whose binding site was detected in the TSG101 promoter was late SV40 factor (LSF). The aim of this study was to analyze the TSG101 protein and LSF expression levels during cervical cancer development. Immunohistochemical analysis confirmed a previously observed decreased expression of TSG101, whereas quantitative polymerase chain reaction (qPCR) and immunohistochemistry analysis revealed high expression of LSF in cervical, precancer and cancer cells compared with human papillomavirus (HPV)‑negative non‑cancer samples. High expression of LSF in cervical cancer HPV‑positive cells suggests that this protein may be important in the regulation of TSG101 expression, as well as in cervical carcinogenesis. The role of LSF as a mediator in cervical cancer development must be confirmed in future studies.
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May-2014
Volume 7 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Broniarczyk JK, Warowicka A, Kwaśniewska A, Wolun‑Cholewa M, Kwaśniewski W and Goździcka‑Józefiak A: Expression of TSG101 protein and LSF transcription factor in HPV‑positive cervical cancer cells. Oncol Lett 7: 1409-1413, 2014
APA
Broniarczyk, J.K., Warowicka, A., Kwaśniewska, A., Wolun‑Cholewa, M., Kwaśniewski, W., & Goździcka‑Józefiak, A. (2014). Expression of TSG101 protein and LSF transcription factor in HPV‑positive cervical cancer cells. Oncology Letters, 7, 1409-1413. https://doi.org/10.3892/ol.2014.1967
MLA
Broniarczyk, J. K., Warowicka, A., Kwaśniewska, A., Wolun‑Cholewa, M., Kwaśniewski, W., Goździcka‑Józefiak, A."Expression of TSG101 protein and LSF transcription factor in HPV‑positive cervical cancer cells". Oncology Letters 7.5 (2014): 1409-1413.
Chicago
Broniarczyk, J. K., Warowicka, A., Kwaśniewska, A., Wolun‑Cholewa, M., Kwaśniewski, W., Goździcka‑Józefiak, A."Expression of TSG101 protein and LSF transcription factor in HPV‑positive cervical cancer cells". Oncology Letters 7, no. 5 (2014): 1409-1413. https://doi.org/10.3892/ol.2014.1967